Ce(IV)-INITIATED GRAFT POLYMERISATION ON POLYMERS CONTAINING NO HYDROXYL GROUPS

Abstract

The invention relates to a process for the preparation of a separating material having improved binding capacity, and to materials prepared and to the use thereof for the separation of charged or uncharged biopolymers from liquids.

1 - 14 . (canceled) 
     
     
         15 . A process for preparing a chromatography material, comprising graft-polymerizing an organic, polymeric base material that is free from OH groups on the surface in the presence of a Ce(IV) catalyst. 
     
     
         16 . A process according to  claim 15 , wherein the polymeric base material is a particulate polymer or shaped polymer which is prepared by polymerisation or copolymerisation of monomers selected from the group consisting of polyvinyl group-containing monomers, divinylbenzene, trivinylbenzene, divinyltoluene, divinylpyridine, divinylnaphthalene, ethylene glycol dimethacrylate, polyethylene glycol dimethacrylate, poly-propylene glycol dimethacrylate, N,N-methylenebisacrylamide, N,N-divinylethyleneurea and N,N-divinylpropyleneurea, monovinyl group-containing monomers, glycidyl methacrylate, methyl methacrylate, ethyl methacrylate, propyl methacrylate, butyl methacrylate, methyl acrylate, ethyl acrylate, propyl acrylate, butyl acrylate, N-vinylpyrrolidone, N-methyl-N-vinylacetamide, acrylonitrile, chlorostyrene and aminostyrene. 
     
     
         17 . A process according to  claim 15 , wherein the base material comprises particles having an average particle diameter in the range from 20 nm to 1000 μm. 
     
     
         18 . A process according to  claim 15 , wherein the base material comprises particles having an average particle surface area in the range from 20 to 1200 m 2 /g. 
     
     
         19 . A process according to  claim 15 , wherein a base polymer in the form of a monolithic body is graft-polymerised. 
     
     
         20 . A process according to  claim 15 , wherein the graft polymerisation is carried out in the presence of ammonium cerium(IV) nitrate as catalyst. 
     
     
         21 . A process according to  claim 15 , wherein the Ce(IV) ion-catalysed reaction is carried out in the presence of at least one inorganic acid selected from the group consisting of nitric acid, sulfuric acid and hydrochloric acid. 
     
     
         22 . A process according to  claim 15 , wherein the graft polymerisation is carried out in a protic solvent. 
     
     
         23 . A process according to  claim 22 , wherein the solvent is water. 
     
     
         24 . A process according to  claim 15 , comprising
 a) dissolving a hydrophilic monomer in water, which is optionally mixed with further monomers,   b) mixing the resultant solution with the support material in such a way that 0.05 to 100 mol of total monomer are present per liter of sedimented polymer material,   c) adding cerium(IV) salt dissolved in mineral acid to the resultant suspension, causing a pH of 0-5, and   d) graft polymerizing the reaction mixture over a period of 0.5 to 72 hours.   
     
     
         25 . A process according to  claim 15 , wherein the base material comprises particles having an average particle surface area in the range from 100 to 600 m 2 /g. 
     
     
         26 . A separating material prepared by a process according to  claim 15 . 
     
     
         27 . A chromatography column containing a separating material according to  claim 26 . 
     
     
         28 . A method for separating materials in a chromatography column, comprising performing the separation in a column containing a separating material prepared by a process according to  claim 15 . 
     
     
         29 . A method for separating biopolymers from liquid media, comprising performing the separation in a column containing a separating material prepared by a process according to  claim 15 .