US2012309021A1PendingUtilityA1

Detection of antibody that binds to water soluble polymer-modified polypeptides

48
Assignee: LUBICH CHRISTIANPriority: May 26, 2009Filed: Jun 6, 2012Published: Dec 6, 2012
Est. expiryMay 26, 2029(~2.9 yrs left)· nominal 20-yr term from priority
Inventors:Christian LubichJuergen SiekmannBirgit Maria ReipertHans-Peter SchwarzHartmut Ehrlich
G01N 33/6854
48
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Claims

Abstract

The present invention provides analytical methods for detecting anti-polymer antibody in an individual. The methods involve contacting a sample from the individual with a water soluble polymer-modified carrier and detecting binding of antibody to the water soluble polymer on the water soluble polymer-modified carrier wherein binding is indicative of the presence of antibody to the water polymer-modified polypeptide. Antibody may be detected to water soluble polymers such as polyethylene glycol, polysialic acid, dextran, hydroxyalkyl starch, or hydroxyethyl starch. When antibody to the water soluble polymer polyethylene glycol is to be detected, the carrier is modified with a non-linear polyethylene glycol derivative.

Claims

exact text as granted — not AI-modified
1 .- 41 . (canceled) 
     
     
         42 . A method of detecting antibodies to a water soluble polymer-modified polypeptide in a sample comprising the steps of:
 a) contacting the sample with a water soluble polymer-modified carrier and   b) detecting binding of antibody to the water soluble polymer on the water soluble polymer-modified carrier,   wherein the water soluble polymer is not PEG or a derivative thereof and wherein binding is indicative of the presence of antibody to the water polymer-modified polypeptide.   
     
     
         43 . The method of  claim 42  wherein the water soluble polymer on the water soluble polymer-modified carrier is a linear water soluble polymer. 
     
     
         44 . The method of  claim 42  wherein the water soluble polymer on the water soluble polymer-modified carrier is a non-linear water soluble polymer. 
     
     
         45 . The method of  claim 42  wherein the polypeptide is a blood coagulation protein. 
     
     
         46 . The method of  claim 45  wherein the water soluble polymer-modified blood coagulation protein is water soluble polymer-modified Factor VIIa. 
     
     
         47 . The method of  claim 45  wherein the water soluble polymer-modified blood coagulation protein is water soluble polymer-modified Factor VIII. 
     
     
         48 . The method of  claim 45  wherein the water soluble polymer-modified blood coagulation protein is water soluble polymer-modified Factor IX. 
     
     
         49 . The method of  claim 42  or  45  wherein the water soluble polymer is a polysaccharide. 
     
     
         50 . The method of  claim 42  or  45  wherein the water soluble polymer is a starch. 
     
     
         51 . The method of  claim 49  wherein the polysaccharide is PSA. 
     
     
         52 . The method of  claim 42  or  45  wherein the water soluble polymer is a linear poly(alkylene oxide), linear poly(vinyl pyrrolidone), linear poly(vinyl alcohol), linear polyoxazoline, or linear poly(acryloylmorpholine). 
     
     
         53 . (canceled)

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