Microdispenser and associated operating method

Abstract

The invention relates to a microdispenser ( 1 ) for dispensing a liquid sample in a dispensing device, with a sample container ( 2 ) for receiving the liquid sample, and with a nozzle ( 7 ) for dispensing the sample located in the sample container ( 2 ). The microdispenser ( 1 ) with the filled sample container ( 2 ) can in this case be stored independently of and fluidically separately from the dispensing device, without the sample escaping from the sample container ( 2 ) during storage.

1 . A microdispenser for dispensing a liquid sample in a dispensing device, comprising:
 a) a sample container for holding the liquid sample;   b) a nozzle for dispensing the sample located in the sample container, wherein the nozzle is adapted to dispense the liquid sample in a form of microdroplets;   c) an actuator for discharging the liquid sample located in the sample container through the nozzle, wherein the actuator is a piezo actuator;   d) a closure adapted for closing the nozzle for storage of the microdispenser and adapted for opening the nozzle for dispensing the sample liquid;   e) a valve for venting the sample container during dispensing of the liquid sample; and   f) a marking containing information about the microdispenser or about the sample located in the sample container, said marking being an optical code,   wherein:   (i) the microdispenser with the sample container containing the liquid sample is adapted to be stored independently of and fluidically separated from the dispensing device, without the sample escaping from the sample container during storage;   (ii) the sample container has an integrated sample reservoir in order to be able to dispense a plurality of samples one after the other without a need for refilling;   (iii) no sample supply line for the microdispenser is provided;   (iv) the nozzle is provided with a coating; and   (v) the coating has an effect of protein-immobilization or DNA-immobilization.   
     
     
         2 . The microdispenser according to  claim 1 , wherein the microdispenser is configured for a single use, and the sample container is not refillable. 
     
     
         3 . The microdispenser according to  claim 1 , wherein the sample container is completely closed apart from the nozzle and has no opening apart from the nozzle. 
     
     
         4 . The microdispenser according to  claim 1 , further comprising a valve for filling the sample container. 
     
     
         5 . The microdispenser according to  claim 1 , wherein a sponge is arranged in the sample container, which sponge generates a holding vacuum in the sample container due to capillary forces acting on the liquid sample. 
     
     
         6 . The microdispenser according to  claim 1 , wherein an inside of the sample container has a stepped cross-section. 
     
     
         7 . The microdispenser according to  claim 6 , wherein the stepped cross-section is a narrowing of the cross-section. 
     
     
         8 . The microdispenser according to  claim 6 , wherein the stepped cross-section is a widening of the cross-section. 
     
     
         9 . The microdispenser according to  claim 1 , wherein the sample container is made of a material selected from the group consisting of plastic, glass and ceramic. 
     
     
         10 . The microdispenser according to  claim 1 , wherein the nozzle is made of a material selected from the group consisting of plastic, glass and ceramic. 
     
     
         11 . The microdispenser according to  claim 10 , wherein the actuator is structurally integrated in the microdispenser. 
     
     
         12 . The microdispenser according to  claim 10 , wherein the actuator is structurally separate from the microdispenser. 
     
     
         13 . The microdispenser according to  claim 10 , wherein the actuator surrounds an outside of the sample container as a sleeve and compresses and expands the sample container upon electrical actuation of the actuator. 
     
     
         14 . The microdispenser according to  claim 10 , wherein the actuator is fixed on the sample container by way of a mounting sleeve. 
     
     
         15 . The microdispenser according to  claim 14 , wherein the mounting sleeve has a spring slot, which runs in an axial direction. 
     
     
         16 . The microdispenser according to  claim 14 , wherein the mounting sleeve is fixed on the sample container by screwing. 
     
     
         17 . The microdispenser according to  claim 14 , wherein the mounting sleeve is fixed on the sample container by a securing ring. 
     
     
         18 . The microdispenser according to  claim 1 , wherein the closure is closed for storage purposes by a member selected from the group consisting of a stopper, a closure film, a wax and a closure cap. 
     
     
         19 . The microdispenser according to  claim 1 , wherein the sample container has a volume in a range from 1 nl to 1 ml. 
     
     
         20 . The microdispenser according to  claim 1 , wherein the nozzle has a nozzle diameter in a range from 10 μm to 100 μm. 
     
     
         21 . The microdispenser according to  claim 1 , wherein the sample container has a working chamber in addition to the sample reservoir, wherein the sample reservoir has a larger volume than the working chamber. 
     
     
         22 . A dispensing device comprising at least one microdispenser according to  claim 1 . 
     
     
         23 . An operating method for a microdispenser according to  claim 1 , said method comprising the following steps:
 (a) closing the nozzle prior to storage of the microdispenser;   (b) providing the nozzle with said coating, wherein the coating has an effect of protein-immobilization or DNA-immobilization;   (c) marking the microdispenser with said optical code containing information about the microdispenser or about the sample located in the sample container;   (d) storing the microdispenser comprising the sample container containing the liquid sample separately from the dispensing device;   (e) reading information about the microdispenser or about the sample located in the sample container from the optical code;   (f) opening the nozzle after storage and before dispensing the sample;   (g) dispensing the liquid sample in the form of microdroplets; and   (h) venting the sample container through a valve during the dispensing step.   
     
     
         24 . The operating method according to  claim 23 , wherein the sample container is not refilled and the microdispenser is disposed of after at least one dispensing operation. 
     
     
         25 . The operating method according to  claim 23 , wherein:
 (i) information about the sample located in the sample container is read from a marking attached to the microdispenser;   (ii) the information is read from the marking during storage of the microdispenser; and   (iii) the microdispenser is removed from storage or remains in storage depending on the information that is read.   
     
     
         26 . The operating method according to  claim 23 , wherein the microdispenser is returned to storage after a dispensing operation. 
     
     
         27 . The operating method according to  claim 23 , wherein the sample container is filled with the liquid sample through the nozzle by capillary action. 
     
     
         28 . The operating method according to  claim 23 , wherein the sample container is filled with the liquid sample through a valve.