Macromolecular compound for detecting target material and method of detecting target material by using the same

Abstract

Macromolecular compound for detecting a target material in a biological sample, and a method of using same.

1 . A macromolecular compound for detecting a target material, the macromolecular compound comprising a water-soluble copolymer comprising a first repeating unit to which a dye is bonded and a second repeating unit to which a probe is bonded. 
     
     
         2 . The macromolecular compound of  claim 1 , wherein the first repeating unit and the second repeating unit are each independently acrylamide, poly-L-lysine, N-vinylpyrrolidone, N-acryloxysuccinimide, or pyrollo. 
     
     
         3 . The macromolecular compound of  claim 1 , wherein the water-soluble copolymer comprises the first repeating unit to which the dye is bonded and the second repeating unit to which the probe at a molar ratio of between about 2:8 and about 8:2. 
     
     
         4 . The macromolecular compound of  claim 1 , wherein the water-soluble copolymer of the macromolecular compound further comprises a third repeating unit. 
     
     
         5 . The macromolecular compound of  claim 4 , wherein the third repeating unit is selected from the group consisting of acrylamide, poly-L-lysine, N-vinylpyrrolidone, N-acryloxysuccinimide, and pyrollo. 
     
     
         6 . The macromolecular compound of  claim 4 , wherein the third repeating unit and the first and second repeating units to which the dye and probe are respectively bonded are irregularly arranged at a molar ratio of between 2:8 and 8:2. 
     
     
         7 . The macromolecular compound of  claim 1 , wherein the water-soluble copolymer has a molecular weight of about 1,000 to about 1,000,000. 
     
     
         8 . The macromolecular compound of  claim 1 , wherein the dye comprises at least one of a photochromic dye, an electrochromic dye, a thermochromic dye, or an indicator dye. 
     
     
         9 . The macromolecular compound of  claim 1 , wherein the target material comprises a polynucleotide, protein, lipid, or carbohydrate. 
     
     
         10 . The macromolecular compound of  claim 1 , wherein the macromolecular compound comprises two or more probes that specifically bond to different sites of the target material. 
     
     
         11 . The macromolecular compound of  claim 1 , wherein the probe comprises a polynucleotide or antibody. 
     
     
         12 . A method of detecting a target material, the method comprising:
 contacting a biological sample, which comprises a target material, with a macromolecular compound comprising a water-soluble copolymer comprising a first repeating unit to which a dye is bonded and a second repeating unit to which a probe for the target material is bonded; and   identifying a location of the target material in the biological sample in view of color of the macromolecular compound that is specifically bound to the target material.   
     
     
         13 . The method of  claim 12 , wherein the biological sample is a cell or tissue. 
     
     
         14 . The method of  claim 12 , wherein the method further comprises, after contacting the biological sample with the macromolecular compound, changing color of the macromolecular compound. 
     
     
         15 . The method of  claim 14 , wherein changing of the color of the macromolecular compound comprises changing the pH of a solution comprising the macromolecular compound, electrically stimulating the macromolecular compound, changing the temperature of a solution including the macromolecular compound, or optically stimulating the macromolecular compound.