Reaction chip, reaction method, temperature controlling unit for gene treating apparatus and gene treating apparatus

Abstract

The reaction chip of the present invention has a plurality of recesses 6 constituting a part of a reaction container and a groove constituting a part of a channel formed on at least one of one face of a first base material (resin base material 2 ) and one face of a second base material (metallic base material) and a notch 15 showing a gradual increase in width and a gradual increase in depth from one face 2 d of the base material toward an inner wall surface 6 d of the recess is formed on an edge of at least one recess in an extending direction of the groove. One face of the first base material and one face of the second base material are stuck together opposite to each other to form the plurality of reaction containers and the channel.

1 . A reaction method using a reaction chip having a plurality of reaction containers constituted by a pair of base materials and a channel that mutually communicates with the plurality of reaction containers, the method comprising the steps of:
 arranging a reagent inside a recess of a first base material of the pair of base materials, the recess constituting a part of one of the reaction containers formed in the first base material;   sealing the reagent with a hot-melt sealing compound;   producing the reaction chip, having the reaction containers in which the reagent is arranged and the channel, by sticking together the first base material and a second base material of the pair of base materials, the second base material constituted by a material whose thermal conductivity is higher than that of the first base material;   feeding a reagent solution into the reaction containers through the channel; and   causing a reaction of the reagent and the reagent solution to proceed while heat is added from a side of the second base material, after the reagent and the reagent solution are brought into contact with each other by heating the reaction chip from the side of the second base material to melt the sealing compound.   
     
     
         2 . The reaction method according to  claim 1 , wherein the first base material and the second base material are stuck together through thermal welding of a sealant layer provided on at least one side of the first base material and the second base material by adding heat from the side of the second base material. 
     
     
         3 . The reaction method according to  claim 1 , wherein the recess in the first base material and a recess in the second base material constitute the one reaction container. 
     
     
         4 . The reaction method according to  claim 1 , wherein a resin material is used as the first base material and a metallic material is used as the second base material. 
     
     
         5 . The reaction method according to  claim 1 , wherein the sealing compound is constituted by a material that is soluble in neither the reagent nor the reagent solution. 
     
     
         6 . The reaction method according to  claim 1 , wherein the one reaction container is a reaction container for enzyme reaction.