Separating material and method for collecting cell or the like using the same

Abstract

The present invention provides a novel material useful for selectively isolating a cell such as monocyte and the like or a protein from a body fluid and a production method thereof, a physiological material using the material and an isolation material using the physiological material, as well as a method of harvesting a cell such as monocyte and the like using the isolation material, a method of harvesting a protein and a method of preparing a dendritic cell.

1 . A method of harvesting a cell comprising performing
 1) a step for trapping a cell on an isolation material by contacting a body fluid with the isolation material comprising, on at least a part of a surface thereof, a substance having a sugar structure,   2) a step for removing untrapped cells by washing the isolation material with a washing liquid, and   3) a step for collecting a trapped cell from the isolation material, in this order, wherein the substance having a sugar structure is the polyurethane represented by the following formula [1-1]:   
       
         
           
           
               
               
           
         
         wherein 
         R 1  is a divalent C 1-16  hydrocarbon group optionally having substituent(s), 
         R 2  is a divalent group containing the same or different 1 to 100 units in total selected from a C 2-12  oxyalkylene unit and a divalent C 2-12  hydrocarbon unit optionally having substituent(s), 
         R 3  is a C 2-4  alkylene group, 
         R 4  is a monosaccharide- or disaccharide-derived sugar residue, 
         OLS is an oligosaccharide skeleton having two primary hydroxyl groups, 
         r shows the total number of secondary hydroxyl groups of oligosaccharide, 
         p and q are each an integer within the range of 1≦p≦r, 0≦q≦r−1, and 
         m and n are each a repeat unit number, m is an integer of 0-1000 and n is an integer of 1-1000, and n/(m+n) is a number within the range of 0.01-1.00, 
         the following formula [1-2]: 
       
       
         
           
           
               
               
           
         
         wherein 
         R 1  is a divalent C 1-16  hydrocarbon group optionally having substituent(s), 
         R 2  is a divalent group containing the same or different 1 to 100 units in total selected from a C 2-12  oxyalkylene unit and a divalent C 2-12  hydrocarbon unit optionally having substituent(s), 
         R 3  is a C 2-4  alkylene group, 
         OLS is an oligosaccharide skeleton having two primary hydroxyl groups, 
         r shows the total number of secondary hydroxyl groups of oligosaccharide, 
         p and q are each an integer within the range of 1≦p≦r, 0≦q≦r−1, and 
         m and n are each a repeat unit number, m is an integer of 0-1000 and n is an integer of 1-1000, and n/(m+n) is a number within the range of 0.01-1.00, 
         the following formula [2-1]: 
       
       
         
           
           
               
               
           
         
         wherein 
         R 1  is a divalent C 1-16  hydrocarbon group optionally having substituent(s), 
         R 2  is a divalent group containing the same or different 1 to 100 units in total selected from a C 2-12  oxyalkylene unit and a divalent C 2-12  hydrocarbon unit optionally having substituent(s), 
         OLS is an oligosaccharide skeleton having two primary hydroxyl groups, 
         r shows the total number of secondary hydroxyl groups of oligosaccharide, 
         XM is an acid-containing group wherein M is a hydrogen atom, an alkali metal atom, an ammonium group or an organic amino group, and X is an acid-derived moiety, 
         s shows the number of the secondary hydroxyl groups of an oligosaccharide modified with an acid-containing group, which is an integer within the range of 1≦s≦r, and 
         m and n are each a repeat unit number, m is an integer of 0-1000 and n is an integer of 1-1000, and n/(m+n) is a number within the range of 0.01-1.00, or 
         the following formula [3-1]: 
       
       
         
           
           
               
               
           
         
         wherein 
         R 1  is a divalent C 1-16  hydrocarbon group optionally having substituent(s), 
         R 2  is a divalent group containing the same or different 1 to 100 units in total selected from a C 2-12  oxyalkylene unit and a divalent C 2-12  hydrocarbon unit optionally having substituent(s), 
         R 3  is a C 2-4  alkylene group, 
         R 4  is a monosaccharide- or disaccharide-derived sugar residue, 
         XM is an acid-containing group wherein M is a hydrogen atom, an alkali metal atom, an ammonium group or an organic amino group, and X is an acid-derived moiety, s shows the number of hydroxyl groups of a sugar residue R 4  modified with an acid-containing group, which is an integer within the range of 1≦s≦4, 
         OLS is an oligosaccharide skeleton having two primary hydroxyl groups, 
         r shows the total number of secondary hydroxyl groups of oligosaccharide, 
         p and q are each an integer within the range of 1≦p≦r, 0≦q≦r−1, and 
         m and n are each a repeat unit number, m is an integer of 0-1000 and n is an integer of 1-1000, and n/(m+n) is a number within the range of 0.01-1.00.