Reaction chip, reaction method, temperature controlling unit for gene treating apparatus and gene treating apparatus

Abstract

The reaction chip of the present invention has a plurality of recesses 6 constituting a part of a reaction container and a groove constituting a part of a channel formed on at least one of one face of a first base material (resin base material 2 ) and one face of a second base material (metallic base material) and a notch 15 showing a gradual increase in width and a gradual increase in depth from one face 2 d of the base material toward an inner wall surface 6 d of the recess is formed on an edge of at least one recess in an extending direction of the groove. One face of the first base material and one face of the second base material are stuck together opposite to each other to form the plurality of reaction containers and the channel.

1 . A reaction chip comprising: a plurality of reaction containers to cause a reaction between a reagent and a reagent solution the reaction containers constituted by a first base material and a second base material; and a channel that mutually communicates with the plurality of reaction containers to feed the reagent solution to the plurality of reaction containers,
 wherein a plurality of recesses of the plurality of reaction containers is formed on at least one face from among a face of the first base material and a face of the second base material;   wherein a groove of the channel is formed at a position corresponding to between the recesses of the plurality of recesses;   wherein a notch is formed on an edge of at least one recess of the plurality of recesses, the notch formed in an extending direction of the groove, the notch showing a gradual increase in width and a gradual increase in depth from the face of the base material where the at least one recess is formed toward an inner wall surface of the at least one recess; and   wherein the plurality of reaction containers and the channel are formed by the face of the first base material and the face of the second base material being stuck together opposite to each other.   
     
     
         2 . The reaction chip according to  claim 1 , wherein an angle formed by the at least one face and an inner wall surface of the notch is smaller than an angle formed by the face of the base material where the at least one recess is formed and the inner wall surface of the at least one recess. 
     
     
         3 . The reaction chip according to  claim 1 , wherein the notch is formed on at least an inflow side of the reagent solution flowing through the groove on the edge of the at least one recess. 
     
     
         4 . The reaction chip according to  claim 1 , wherein the at least one recess has a columnar space having an inner wall surface at substantially right angles to the face of the base material where the at least one recess is formed, the inner wall surface of the columnar space disposed on at least an opening side, and
 the maximum depth on the edge of the notch is shallower than the depth of the columnar space.   
     
     
         5 . The reaction chip according to  claim 1 , wherein an outer shape of the at least one recess is circular in plane view and a plane shape of the notch is defined by an area inside two tangents drawn from one point to a circle forming an outer edge of the at least one recess, the one point is on the face of the base material where the at least one recess is formed, and the one point is in the extending direction of the groove. 
     
     
         6 . The reaction chip according to  claim 1 , wherein a center line in the extending direction of the groove of the notch is aligned with a center line of the groove on a same straight line.