Methods of identifying individuals at risk of perioperative bleeding, renal dysfunction or stroke

Abstract

The present invention relates, in general, to perioperative bleeding and, in particular, to methods of identifying individuals at risk of perioperative bleeding. The present invention relates, in general, to perioperative renal dysfunction and, in particular, to methods of identifying individuals at risk of perioperative renal dysfunction. The present invention relates, in general, to perioperative stroke and, in particular, to methods of identifying individuals at risk of perioperative stroke.

1 . A method of identifying a patient at risk of perioperative or periprocedure bleeding comprising assaying DNA from said patient for a polymorphism in at least one gene selected from the group consisting of the α 2 β 1  integrin gene, the GPIbα gene, the tissue factor (TF) gene, the prothrombin gene, tissue factor pathway inhibitor (TFPI) gene, and the angiotensin converting enzyme (ACE) gene,
 wherein the presence of an α 2 β 1  integrin −52C/T or 807C/T polymorphism, a GPIbα 524C/T polymorphism, a TF −603A/G polymorphism, a prothrombin 20210G/A polymorphism, a TFPI −399C/T polymorphism or an ACE intron 16 deletion/insertion polymorphism is associated with risk of said bleeding. 
 
     
     
         2 . A method of identifying a patient at risk of perioperative or periprocedure renal dysfunction comprising assaying DNA from said patient for a polymorphism in at least one gene selected from the group consisting of the interleukin-6 (IL6) gene, the angiotensinogen (AGT) gene, the apolipoproteinE (APOE) (ε2) gene, the angiotensin receptor1 (AGTR1) gene, the endothelial nitric oxide synthase (eNOS) gene and the angiotensin converting enzyme (ACE) gene,
 wherein the presence of an IL6 G-572C polymorphism, an AGT T842C polymorphism, an APOE (ε2) C586T polymorphism, an AGTR1 A1166C polymorphism, an eNOS G894T polymorphism or an ACE deletion/insertion polymorphism is associated with risk of said renal dysfunction 
 
     
     
         3 . A method of identifying a patient at risk of perioperative or periprocedure stroke comprising assaying DNA from said patient for polymorphisms in the C-Reactive Protein (CRP) and Interleukin-6 (IL6) genes, wherein the presence of CPR 3′ UTR. 1846 C/T and IL6 −1740/C polymorphisms is associated with risk of said stroke. 
     
     
         4 . A kit comprising a probe or primer suitable for use in detecting a polymorphism of  claim 1 . 
     
     
         5 . A kit comprising a probe or primer suitable for use in detecting a polymorphism of  claim 2 . 
     
     
         6 . A kit comprising a probe or primer suitable for use in detecting a polymorphism of  claim 3 .