G-csf protein having a deletion of an amino acid sequence corresponding to exon 3 for use as a diagnostic cancer marker

Abstract

Disclosed are a method, a composition, a microarray, an antibody and a kit for diagnosis and prognosis of cancer, based on detection of deletion of the exon 3 region of G-CSF gene or levels of a mutated G-CSF protein having a deletion of an amino acid sequence corresponding to the exon 3 region, wherein the deletion of the exon 3 region of the G-CSF gene is used as a cancer biomarker.

1 . (canceled) 
     
     
         2 . A mutated G-CSF protein having a deletion of an amino acid sequence corresponding to an exon 3 region for use as a diagnostic cancer marker. 
     
     
         3 - 8 . (canceled) 
     
     
         9 . A mutated G-CSF protein having a deletion of an amino acid sequence corresponding to an exon 3 region. 
     
     
         10 - 14 . (canceled) 
     
     
         15 . A mutated G-CSF mRNA or cDNA fragment having a deletion of an exon 3 region for use as a diagnostic cancer marker. 
     
     
         16 . A primer for use in amplification of a G-CSF gene in a G-CSF nucleic acid sample obtained from mammalian tissues or cells. 
     
     
         17 . The primer as set forth in  claim 16 , wherein said primer is selected from the group consisting of the following nucleotide sequence pairs: 
       
         
           
                 
                 
               
                     
                   sense: 
                 
                     
                   (SEQ ID NO. 1) 
                 
                     
                   5′-ACCCCCCTGGGCCCTGCC-3′ 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   antisense: 
                 
                     
                   (SEQ ID NO. 2) 
                 
                     
                   5′-TCAGGGCTGGGCAAGGTG-3′; 
                 
                     
                     
                 
                     
                   sense: 
                 
                     
                   (SEQ ID NO. 1) 
                 
                     
                   5′-ACCCCCCTGGGCCCTGCC-3′ 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   antisense: 
                 
                     
                   (SEQ ID NO. 5) 
                 
                     
                   5′-CAGCTGCAGGGCCTGGCT-3′; 
                 
                     
                     
                 
                     
                   sense: 
                 
                     
                   (SEQ ID NO. 1) 
                 
                     
                   5′-ACCCCCCTGGGCCCTGCC-3′ 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   antisense: 
                 
                     
                   (SEQ ID NO. 6) 
                 
                     
                   5′-CGCTATGGAGTTGGCTCAAGC-3′; 
                 
                     
                     
                 
                     
                   sense: 
                 
                     
                   (SEQ ID NO. 1) 
                 
                     
                   5′-ACCCCCCTGGGCCCTGCC-3′ 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   antisense: 
                 
                     
                   (SEQ ID NO. 9) 
                 
                     
                   5′-CAGCTTCTCCTGGAGCGC-3′; 
                 
                     
                     
                 
                     
                   sense: 
                 
                     
                   (SEQ ID NO. 3) 
                 
                     
                   5′-ATCCAGGGCGATGGCGCAGCG-3′ 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   antisense: 
                 
                     
                   (SEQ ID NO. 2) 
                 
                     
                   5′-TCAGGGCTGGGCAAGGTG-3′; 
                 
                     
                     
                 
                     
                   sense: 
                 
                     
                   (SEQ ID NO. 3) 
                 
                     
                   5′-ATCCAGGGCGATGGCGCAGCG-3′ 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   antisense: 
                 
                     
                   (SEQ ID NO. 5) 
                 
                     
                   5′-CAGCTGCAGGGCCTGGCT-3′; 
                 
                     
                     
                 
                     
                   sense: 
                 
                     
                   (SEQ ID NO. 3) 
                 
                     
                   5′-ATCCAGGGCGATGGCGCAGCG-3′ 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   antisense: 
                 
                     
                   (SEQ ID NO. 6) 
                 
                     
                   5′-CGCTATGGAGTTGGCTCAAGC-3′; 
                 
                     
                     
                 
                     
                   sense: 
                 
                     
                   (SEQ ID NO. 4) 
                 
                     
                   5′-TGTGCCACCTACAAGCTGTGC-3′ 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   antisense: 
                 
                     
                   (SEQ ID NO. 2) 
                 
                     
                   5′-TCAGGGCTGGGCAAGGTG-3′; 
                 
                     
                     
                 
                     
                   sense: 
                 
                     
                   (SEQ ID NO. 4) 
                 
                     
                   5′-TGTGCCACCTACAAGCTGTGC-3′ 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   antisense: 
                 
                     
                   (SEQ ID NO. 5) 
                 
                     
                   5′-CAGCTGCAGGGCCTGGCT-3′; 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   sense: 
                 
                     
                   (SEQ ID NO. 4) 
                 
                     
                   5′-TGTGCCACCTACAAGCTGTGC-3′ 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   antisense: 
                 
                     
                   (SEQ ID NO. 6) 
                 
                     
                   5′-CGCTATGGAGTTGGCTCAAGC-3′. 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         18 . A diagnostic method for cancer comprising the steps of: (a) obtaining a G-CSF nucleic acid sample from mammalian tissues or cells; (b) amplifying a G-CSF region from the nucleic acid sample obtained; (c) detecting a deletion of exon 3 of said G-CSF gene in the amplified sample.