Modulating plant oil levels

Abstract

Methods and materials for modulating (e.g., increasing or decreasing) oil levels in plants are disclosed. For example, nucleic acids encoding oil-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased oil levels and plant products produced from plants having increased oil levels.

1 . A method of modulating the level of oil in a plant, said method comprising introducing into a plant cell an isolated nucleic acid comprising a nucleotide sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:81-92, SEQ ID NO:94, SEQ ID NOs:96-107, SEQ ID NO:109, SEQ ID NOs:111-116, SEQ ID NOs:118-134, SEQ ID NOs: 137-140, SEQ ID NO:142, SEQ ID NO:144, SEQ ID NOs:146-147, SEQ ID NOs:149-150, SEQ ID NO:152, SEQ ID NO:154, SEQ ID NOs:156-157, SEQ ID NOs:159-169, SEQ ID NOs:171-172, SEQ ID NO:174, SEQ ID NO:176, SEQ ID NOs:178-179, SEQ ID NOs:181-182, SEQ ID NOs:184-191, SEQ ID NOs:193-196, SEQ ID NOs:198-217, SEQ ID NOs:219-220, SEQ ID NOs:222-229, SEQ ID NOs:231-238, SEQ ID NO:240, SEQ ID NOs:242-250, SEQ ID NOs:252-330, SEQ ID NOs:332-333, SEQ ID NO:335, SEQ ID NOs:337-340, SEQ ID NO:342, SEQ ID NO:344, SEQ ID NO:346-348, SEQ ID NOs:350-357, SEQ ID NOs:359-366, SEQ ID NO:368, SEQ ID NOs:370-372, SEQ ID NOs:374-376, SEQ ID NOs:378-379, SEQ ID NOs:381-396, SEQ ID NO:398, and SEQ ID NOs:502-545, wherein a tissue of a plant produced from said plant cell has a difference in the level of oil as compared to the corresponding level in tissue of a control plant that does not comprise said nucleic acid. 
     
     
         2 . The method of  claim 1 , said nucleotide sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:81-82, SEQ ID NOs:84-85, SEQ ID NO:87, SEQ ID NO:91, SEQ ID NO:94, SEQ ID NOs:96-101, SEQ ID NOs:103-105, SEQ ID NO:109, SEQ ID NO:111, SEQ ID NOs:113-115, SEQ ID NO:118, SEQ ID NOs:120-121, SEQ ID NOs:125-132, SEQ ID NO:134, SEQ ID NOs:139-140, SEQ ID NO:142, SEQ ID NO:144, SEQ ID NOs:146-147, SEQ ID NO:149, SEQ ID NO:152, SEQ ID NO:154, SEQ ID NOs:156-157, SEQ ID NO:159, SEQ ID NOs:162-168, SEQ ID NOs:171-172, SEQ ID NO:174, SEQ ID NO:176, SEQ ID NO:178, SEQ ID NO:181, SEQ ID NO:184, SEQ ID NOs:193-194, SEQ ID NO:198, SEQ ID NO:207, SEQ ID NOs:215-217, SEQ ID NOs:219-220, SEQ ID NOs:222-223, SEQ ID NOs:231-232, SEQ ID NO:235, SEQ ID NO:238, SEQ ID NO:240, SEQ ID NO:242, SEQ ID NOs:244-246, SEQ ID NO:250, SEQ ID NO:252, SEQ ID NO:254, SEQ ID NOs:257-258, SEQ ID NO:261, SEQ ID NOs:265-267, SEQ ID NO:270, SEQ ID NO:279, SEQ ID NOs:287-293, SEQ ID NOs:300-301, SEQ ID NO:304, SEQ ID NOs:307-309, SEQ ID NOs:311-313, SEQ ID NOs:317-318, SEQ ID NO:320, SEQ ID NOs:323-324, SEQ ID NO:327, SEQ ID NO:332, SEQ ID NO:335, SEQ ID NOs:337-340, SEQ ID NO:342, SEQ ID NO:344, SEQ ID NOs:346-348, SEQ ID NOs:350-351, SEQ ID NOs:355-357, SEQ ID NOs:359-361, SEQ ID NOs:363-366, SEQ ID NO:368, SEQ ID NOs:370-372, SEQ ID NO:374, SEQ ID NO:378, SEQ ID NO:381, SEQ ID NO:398, SEQ ID NOs:502-505, SEQ ID NOs:507-519, SEQ ID NO:521, SEQ ID NOs:524-527, SEQ ID NOs:529-534, SEQ ID NO:541, and SEQ ID NOs:544-545, wherein a tissue of a plant produced from said plant cell has a difference in the level of oil as compared to the corresponding level in tissue of a control plant that does not comprise said nucleic acid. 
     
     
         3 . (canceled) 
     
     
         4 . The method of  claim 1 , wherein said sequence identity is 85 percent or greater. 
     
     
         5 . The method of  claim 4 , wherein said sequence identity is 90 percent or greater. 
     
     
         6 . The method of  claim 4 , wherein said sequence identity is 95 percent or greater. 
     
     
         7 . The method of  claim 1 , wherein said nucleotide sequence encodes a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:81, SEQ ID NO:94, SEQ ID NO:111, SEQ ID NO:152, SEQ ID NO:159, SEQ ID NO:171, SEQ ID NO:176, SEQ ID NO:178, SEQ ID NO:193, SEQ ID NO:332, SEQ ID NO:342, SEQ ID NO:344, SEQ ID NO:346, SEQ ID NO:359, SEQ ID NO:374, and SEQ ID NO:398. 
     
     
         8 . The method of  claim 1 , wherein said difference is an increase in the level of oil. 
     
     
         9 . The method of  claim 1 , wherein said isolated nucleic acid is operably linked to a regulatory region. 
     
     
         10 . The method of  claim 9 , wherein said regulatory region is a tissue-preferential promoter or a broadly expressing promoter. 
     
     
         11 . (canceled) 
     
     
         12 . (canceled) 
     
     
         13 . The method of  claim 1 , wherein said plant is a dicot. 
     
     
         14 . The method of  claim 13 , wherein said plant is a member of the genus  Anacardium, Arachis, Azadirachta, Brassica, Cannabis, Carthamus, Corylus, Crambe, Cucurbita, Glycine, Gossypium, Helianthus, Jatropha, Juglans, Linum, Olea, Papaver, Persea, Prunus, Ricinus, Sesamum, Simmondsia , or  Vitis.    
     
     
         15 . The method of  claim 1 , wherein said plant is a monocot. 
     
     
         16 . The method of  claim 15 , wherein said plant is a member of the genus  Cocos, Elaeis, Oryza , or  Zea.    
     
     
         17 . The method of  claim 1 , wherein said tissue is seed tissue. 
     
     
         18 . A method of producing a plant tissue, said method comprising growing a plant cell comprising an exogenous nucleic acid comprising a nucleotide sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:81-92, SEQ ID NO:94, SEQ ID NOs:96-107, SEQ ID NO:109, SEQ ID NOs:111-116, SEQ ID NOs:118-134, SEQ ID NOs: 137-140, SEQ ID NO:142, SEQ ID NO:144, SEQ ID NOs:146-147, SEQ ID NOs:149-150, SEQ ID NO:152, SEQ ID NO:154, SEQ ID NOs:156-157, SEQ ID NOs:159-169, SEQ ID NOs:171-172, SEQ ID NO:174, SEQ ID NO:176, SEQ ID NOs:178-179, SEQ ID NOs:181-182, SEQ ID NOs:184-191, SEQ ID NOs:193-196, SEQ ID NOs:198-217, SEQ ID NOs:219-220, SEQ ID NOs:222-229, SEQ ID NOs:231-238, SEQ ID NO:240, SEQ ID NOs:242-250, SEQ ID NOs:252-330, SEQ ID NOs:332-333, SEQ ID NO:335, SEQ ID NOs:337-340, SEQ ID NO:342, SEQ ID NO:344, SEQ ID NO:346-348, SEQ ID NOs:350-357, SEQ ID NOs:359-366, SEQ ID NO:368, SEQ ID NOs:370-372, SEQ ID NOs:374-376, SEQ ID NOs:378-379, SEQ ID NOs:381-396, SEQ ID NO:398, and SEQ ID NOs:502-545, wherein said tissue has a difference in the level of oil as compared to the corresponding level in tissue of a control plant that does not comprise said nucleic acid. 
     
     
         19 . The method of  claim 18 , said nucleotide sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:81-82, SEQ ID NOs:84-85, SEQ ID NO:87, SEQ ID NO:91, SEQ ID NO:94, SEQ ID NOs:96-101, SEQ ID NOs:103-105, SEQ ID NO:109, SEQ ID NO:111, SEQ ID NOs:113-115, SEQ ID NO:118, SEQ ID NOs:120-121, SEQ ID NOs:125-132, SEQ ID NO:134, SEQ ID NOs:139-140, SEQ ID NO:142, SEQ ID NO:144, SEQ ID NOs:146-147, SEQ ID NO:149, SEQ ID NO:152, SEQ ID NO:154, SEQ ID NOs:156-157, SEQ ID NO:159, SEQ ID NOs:162-168, SEQ ID NOs:171-172, SEQ ID NO:174, SEQ ID NO:176, SEQ ID NO:178, SEQ ID NO:181, SEQ ID NO:184, SEQ ID NOs:193-194, SEQ ID NO:198, SEQ ID NO:207, SEQ ID NOs:215-217, SEQ ID NOs:219-220, SEQ ID NOs:222-223, SEQ ID NOs:231-232, SEQ ID NO:235, SEQ ID NO:238, SEQ ID NO:240, SEQ ID NO:242, SEQ ID NOs:244-246, SEQ ID NO:250, SEQ ID NO:252, SEQ ID NO:254, SEQ ID NOs:257-258, SEQ ID NO:261, SEQ ID NOs:265-267, SEQ ID NO:270, SEQ ID NO:279, SEQ ID NOs:287-293, SEQ ID NOs:300-301, SEQ ID NO:304, SEQ ID NOs:307-309, SEQ ID NOs:311-313, SEQ ID NOs:317-318, SEQ ID NO:320, SEQ ID NOs:323-324, SEQ ID NO:327, SEQ ID NO:332, SEQ ID NO:335, SEQ ID NOs:337-340, SEQ ID NO:342, SEQ ID NO:344, SEQ ID NOs:346-348, SEQ ID NOs:350-351, SEQ ID NOs:355-357, SEQ ID NOs:359-361, SEQ ID NOs:363-366, SEQ ID NO:368, SEQ ID NOs:370-372, SEQ ID NO:374, SEQ ID NO:378, SEQ ID NO:381, SEQ ID NO:398, SEQ ID NOs:502-505, SEQ ID NOs:507-519, SEQ ID NO:521, SEQ ID NOs:524-527, SEQ ID NOs:529-534, SEQ ID NO:541, and SEQ ID NOs:544-545, wherein said tissue has a difference in the level of oil as compared to the corresponding level in tissue of a control plant that does not comprise said nucleic acid. 
     
     
         20 . (canceled) 
     
     
         21 . The method of  claim 18 , wherein said sequence identity is 85 percent or greater. 
     
     
         22 . The method of  claim 21 , wherein said sequence identity is 90 percent or greater. 
     
     
         23 . The method of  claim 21 , wherein said sequence identity is 95 percent or greater. 
     
     
         24 . The method of  claim 18 , wherein said nucleotide sequence encodes a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:81, SEQ ID NO:94, SEQ ID NO:111, SEQ ID NO:152, SEQ ID NO:159, SEQ ID NO:171, SEQ ID NO:176, SEQ ID NO:178, SEQ ID NO:193, SEQ ID NO:332, SEQ ID NO:342, SEQ ID NO:344, SEQ ID NO:346, SEQ ID NO:359, SEQ ID NO:374, and SEQ ID NO:398. 
     
     
         25 . The method of  claim 18 , wherein said difference is an increase in the level of oil. 
     
     
         26 . The method of  claim 18 , wherein said exogenous nucleic acid is operably linked to a regulatory region. 
     
     
         27 . The method of  claim 26 , wherein said regulatory region is a tissue-preferential promoter or a broadly expressing promoter. 
     
     
         28 . (canceled) 
     
     
         29 . (canceled) 
     
     
         30 . The method of  claim 18 , wherein said plant tissue is dicotyledonous. 
     
     
         31 . The method of  claim 30 , wherein said plant tissue is a member of the genus  Anacardium, Arachis, Azadirachta, Brassica, Cannabis, Carthamus, Corylus, Crambe, Cucurbita, Glycine, Gossypium, Helianthus, Jatropha, Juglans, Linum, Olea, Papaver, Persea, Prunus, Ricinus, Sesamum, Simmondsia , or  Vitis.    
     
     
         32 . The method of  claim 18 , wherein said plant tissue is monocotyledonous. 
     
     
         33 . The method of  claim 32 , wherein said plant tissue is a member of the genus  Cocos, Elaeis, Oryza , or  Zea.    
     
     
         34 . The method of  claim 18 , wherein said tissue is seed tissue. 
     
     
         35 . A plant cell comprising an exogenous nucleic acid comprising a nucleotide sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:81-92, SEQ ID NO:94, SEQ ID NOs:96-107, SEQ ID NO:109, SEQ ID NOs:111-116, SEQ ID NOs:118-134, SEQ ID NOs: 137-140, SEQ ID NO:142, SEQ ID NO:144, SEQ ID NOs:146-147, SEQ ID NOs:149-150, SEQ ID NO:152, SEQ ID NO:154, SEQ ID NOs:156-157, SEQ ID NOs:159-169, SEQ ID NOs:171-172, SEQ ID NO:174, SEQ ID NO:176, SEQ ID NOs:178-179, SEQ ID NOs:181-182, SEQ ID NOs:184-191, SEQ ID NOs:193-196, SEQ ID NOs:198-217, SEQ ID NOs:219-220, SEQ ID NOs:222-229, SEQ ID NOs:231-238, SEQ ID NO:240, SEQ ID NOs:242-250, SEQ ID NOs:252-330, SEQ ID NOs:332-333, SEQ ID NO:335, SEQ ID NOs:337-340, SEQ ID NO:342, SEQ ID NO:344, SEQ ID NO:346-348, SEQ ID NOs:350-357, SEQ ID NOs:359-366, SEQ ID NO:368, SEQ ID NOs:370-372, SEQ ID NOs:374-376, SEQ ID NOs:378-379, SEQ ID NOs:381-396, SEQ ID NO:398, and SEQ ID NOs: 502-545, wherein a tissue of a plant produced from said plant cell has a difference in the level of oil as compared to the corresponding level in tissue of a control plant that does not comprise said nucleic acid. 
     
     
         36 . The plant cell of  claim 35 , said nucleotide sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:81-82, SEQ ID NOs:84-85, SEQ ID NO:87, SEQ ID NO:91, SEQ ID NO:94, SEQ ID NOs:96-101, SEQ ID NOs:103-105, SEQ ID NO:109, SEQ ID NO:111, SEQ ID NOs:113-115, SEQ ID NO:118, SEQ ID NOs:120-121, SEQ ID NOs:125-132, SEQ ID NO:134, SEQ ID NOs:139-140, SEQ ID NO:142, SEQ ID NO:144, SEQ ID NOs:146-147, SEQ ID NO:149, SEQ ID NO:152, SEQ ID NO:154, SEQ ID NOs:156-157, SEQ ID NO:159, SEQ ID NOs:162-168, SEQ ID NOs:171-172, SEQ ID NO:174, SEQ ID NO:176, SEQ ID NO:178, SEQ ID NO:181, SEQ ID NO:184, SEQ ID NOs:193-194, SEQ ID NO:198, SEQ ID NO:207, SEQ ID NOs:215-217, SEQ ID NOs:219-220, SEQ ID NOs:222-223, SEQ ID NOs:231-232, SEQ ID NO:235, SEQ ID NO:238, SEQ ID NO:240, SEQ ID NO:242, SEQ ID NOs:244-246, SEQ ID NO:250, SEQ ID NO:252, SEQ ID NO:254, SEQ ID NOs:257-258, SEQ ID NO:261, SEQ ID NOs:265-267, SEQ ID NO:270, SEQ ID NO:279, SEQ ID NOs:287-293, SEQ ID NOs:300-301, SEQ ID NO:304, SEQ ID NOs:307-309, SEQ ID NOs:311-313, SEQ ID NOs:317-318, SEQ ID NO:320, SEQ ID NOs:323-324, SEQ ID NO:327, SEQ ID NO:332, SEQ ID NO:335, SEQ ID NOs:337-340, SEQ ID NO:342, SEQ ID NO:344, SEQ ID NOs:346-348, SEQ ID NOs:350-351, SEQ ID NOs:355-357, SEQ ID NOs:359-361, SEQ ID NOs:363-366, SEQ ID NO:368, SEQ ID NOs:370-372, SEQ ID NO:374, SEQ ID NO:378, SEQ ID NO:381, SEQ ID NO:398, SEQ ID NOs:502-505, SEQ ID NOs:507-519, SEQ ID NO:521, SEQ ID NOs:524-527, SEQ ID NOs:529-534, SEQ ID NO:541, and SEQ ID NOs:544-545, wherein a tissue of a plant produced from said plant cell has a difference in the level of oil as compared to the corresponding level in tissue of a control plant that does not comprise said nucleic acid. 
     
     
         37 . (canceled) 
     
     
         38 . The plant cell of  claim 35 , wherein said sequence identity is 85 percent or greater. 
     
     
         39 . The plant cell of  claim 38 , wherein said sequence identity is 90 percent or greater. 
     
     
         40 . The plant cell of  claim 38 , wherein said sequence identity is 95 percent or greater. 
     
     
         41 . The plant cell of  claim 37 , wherein said nucleotide sequence encodes a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:81, SEQ ID NO:94, SEQ ID NO:111, SEQ ID NO:152, SEQ ID NO:159, SEQ ID NO:171, SEQ ID NO:176, SEQ ID NO:178, SEQ ID NO:193, SEQ ID NO:332, SEQ ID NO:342, SEQ ID NO:344, SEQ ID NO:346, SEQ ID NO:359, SEQ ID NO:374, and SEQ ID NO:398. 
     
     
         42 . The plant cell of  claim 35 , wherein said difference is an increase in the level of oil. 
     
     
         43 . The plant cell of  claim 35 , wherein said exogenous nucleic acid is operably linked to a regulatory region. 
     
     
         44 . The plant cell of  claim 43 , wherein said regulatory region is a tissue-preferential promoter or a broadly expressing promoter. 
     
     
         45 . (canceled) 
     
     
         46 . (canceled) 
     
     
         47 . The plant cell of  claim 35 , wherein said plant is a dicot. 
     
     
         48 . The plant cell of  claim 47 , wherein said plant is a member of the genus  Anacardium, Arachis, Azadirachta, Brassica, Cannabis, Carthamus, Corylus, Crambe, Cucurbita, Glycine, Gossypium, Helianthus, Jatropha, Juglans, Linum, Olea, Papaver, Persea, Prunus, Ricinus, Sesamum, Simmondsia , or  Vitis.    
     
     
         49 . The plant cell of  claim 35 , wherein said plant is a monocot. 
     
     
         50 . The plant cell of  claim 49 , wherein said plant is a member of the genus  Cocos, Elaeis, Oryza , or  Zea.    
     
     
         51 . The plant cell of  claim 35 , wherein said tissue is seed tissue. 
     
     
         52 . A transgenic plant comprising the plant cell of  claim 35 . 
     
     
         53 . Progeny of the plant of  claim 52 , wherein said progeny has a difference in the level of oil as compared to the level of oil in a corresponding control plant that does not comprise said exogenous nucleic acid. 
     
     
         54 . Seed from a transgenic plant according to  claim 52 . 
     
     
         55 . Vegetative tissue from a transgenic plant according to  claim 52 . 
     
     
         56 . A food or feed product comprising seed or vegetative tissue from a transgenic plant according to  claim 52 . 
     
     
         57 . (canceled) 
     
     
         58 . Oil from the seed of  claim 54 . 
     
     
         59 . A method of making oil, said method comprising extracting oil from the seed of  claim 54 . 
     
     
         60 . An isolated nucleic acid comprising a) a nucleotide sequence having 95% or greater sequence identity to a nucleotide sequence selected from the group consisting of SEQ ID NO:95, SEQ ID NO:108, SEQ ID NO:117, SEQ ID NO:141, SEQ ID NO:143, SEQ ID NO:145, SEQ ID NO:148, SEQ ID NO:153, SEQ ID NO:155, SEQ ID NO:173, SEQ ID NO:180, SEQ ID NO:183, SEQ ID NO:197, SEQ ID NO:218, SEQ ID NO:221, SEQ ID NO:230, SEQ ID NO:239, SEQ ID NO:241, SEQ ID NO:251, SEQ ID NO:334, SEQ ID NO:336, SEQ ID NO:349, SEQ ID NO:367, SEQ ID NO:369, SEQ ID NO:377, SEQ ID NO:380, SEQ ID NO:474, SEQ ID NO:475, SEQ ID NO:476, SEQ ID NO:477, SEQ ID NO:478, SEQ ID NO:479, SEQ ID NO:480, SEQ ID NO:481, SEQ ID NO:482, SEQ ID NO:483, SEQ ID NO:484, SEQ ID NO:485, SEQ ID NO:486, SEQ ID NO:487, SEQ ID NO:488, SEQ ID NO:489, SEQ ID NO:490, SEQ ID NO:491, SEQ ID NO:492, SEQ ID NO:493, SEQ ID NO:494, SEQ ID NO:495, SEQ ID NO:496, SEQ ID NO:497, SEQ ID NO:498, SEQ ID NO:499, SEQ ID NO:500, SEQ ID NO:501, SEQ ID NO:546, SEQ ID NO:547, SEQ ID NO:549, SEQ ID NO:551, SEQ ID NO:553, SEQ ID NO:555, SEQ ID NO:556, SEQ ID NO:557, SEQ ID NO:559, SEQ ID NO:561, SEQ ID NO:562, SEQ ID NO:564, SEQ ID NO:565, SEQ ID NO:566, SEQ ID NO:567, SEQ ID NO:569, SEQ ID NO:570, SEQ ID NO:571, SEQ ID NO:572, SEQ ID NO:573, and SEQ ID NO:575; or b) a nucleotide sequence encoding a polypeptide having 80% or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:96, SEQ ID NO:109, SEQ ID NO:118, SEQ ID NO:142, SEQ ID NO:144, SEQ ID NO:146, SEQ ID NO:149, SEQ ID NO:154, SEQ ID NO:156, SEQ ID NO:174, SEQ ID NO:181, SEQ ID NO:184, SEQ ID NO:198, SEQ ID NO:219, SEQ ID NO:222, SEQ ID NO:231, SEQ ID NO:240, SEQ ID NO:242, SEQ ID NO:252, SEQ ID NO:270, SEQ ID NO:290, SEQ ID NO:292, SEQ ID NO:300, SEQ ID NO:308, SEQ ID NO:309, SEQ ID NO:318, SEQ ID NO:335, SEQ ID NO:337, SEQ ID NO:350, SEQ ID NO:368, SEQ ID NO:370, SEQ ID NO:378, SEQ ID NO:381, SEQ ID NO:502, SEQ ID NO:503, SEQ ID NO:504, SEQ ID NO:505, SEQ ID NO:507, SEQ ID NO:509, SEQ ID NO:511, SEQ ID NO:513, SEQ ID NO:514, SEQ ID NO:515, SEQ ID NO:517, SEQ ID NO:519, SEQ ID NO:521, SEQ ID NO:525, SEQ ID NO:526, SEQ ID NO:527, SEQ ID NO:529, SEQ ID NO:531, SEQ ID NO:532, SEQ ID NO:533, SEQ ID NO:534, SEQ ID NO:541, and SEQ ID NO:545. 
     
     
         61 . (canceled)