Compositions Comprising Tailored Oils

Abstract

Methods and compositions for the production of food compositions, oils, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing food compositions, and transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

1 . An oleaginous microbial triglyceride oil, wherein the fatty acid profile of the triglyceride oil comprises at least 60% C18:1 and less than 7% C18:2. 
     
     
         2 . An oleaginous microbial triglyceride oil of  claim 1 , wherein the oleaginous microbe is a recombinant microbe. 
     
     
         3 . An oleaginous microbial triglyceride oil of  claim 1 , wherein the fatty acid profile of the triglyceride oil has not been altered by further processing the microbial triglyceride oil. 
     
     
         4 . An oleaginous microbial triglyceride oil of  claim 3 , wherein said further processing comprises esterification, distillation, fractionation, crystallization, or precipitation. 
     
     
         5 . An oleaginous microbial triglyceride oil of  claim 4 , wherein the fatty acid profile of the triglyceride oil comprises at least 70% C18:1. 
     
     
         6 . An oleaginous microbial triglyceride oil of  claim 5 , wherein the fatty acid profile of the triglyceride oil comprises at least 80% C18:1. 
     
     
         7 . An oleaginous microbial triglyceride oil of  claim 4 , wherein the fatty acid profile of the triglyceride oil comprises less than 5% C18:2. 
     
     
         8 . An oleaginous microbial triglyceride oil of  claim 7 , wherein the fatty acid profile of the triglyceride oil comprises less than 1% C18:2. 
     
     
         9 . An oleaginous microbial triglyceride oil of  claim 4 , wherein the fatty acid profile of the triglyceride oil comprises at least 80% C18:1 and less than 5% C18:2. 
     
     
         10 . An oleaginous microbial triglyceride oil of  claim 9 , wherein the fatty acid profile of the triglyceride oil comprises at least 80% C18:1 and less than 1% C18:2. 
     
     
         11 . An oleaginous microbial triglyceride oil of  claim 4 , wherein the microbial triglyceride oil is microalgal oil. 
     
     
         12 . An oleaginous microbial triglyceride oil of  claim 11 , wherein the microbial triglyceride oil is  Prototheca  oil or  Chlorella  oil. 
     
     
         13 . An oleagnious microbial triglyceride oil of  claim 12 , wherein the microbial triglyceride oil is  Prototheca moriformis  oil or  Chlorella protothecoides  oil. 
     
     
         14 . A blended triglyceride comprising an oleaginous microbial triglyceride oil of  claim 4  and a triglyceride oil from soy, rapeseed, canola, palm, palm kernel, coconut, corn, waste vegetable, Chinese tallow, olive, sunflower, cottonseed, chicken fat, beef tallow, porcine tallow, microalgae, macroalgae, microbes,  Cuphea , flax, peanut, choice white grease, lard,  Camelina sativa , mustard seed, cashew nut, oats, lupine, kenaf, calendula, help, coffee, linseed (flax), hazelnut, euphorbia, pumpkin seed, coriander, camellia, sesame, safflower, rice, tung tree, cocoa, copra, opium poppy, castor beans, pecan, jojoba, macadamia, Brazil nuts, avocado, petroleum, or a distillate fraction of any of the preceding oils. 
     
     
         15 . A product produced by chemically modifying an oleaginous microbial triglyceride oil, wherein the fatty acid profile of the triglyceride oil comprises at least 60% C18:1 and less than 7% C18:2, and said triglyceride oil is chemically modified by one or more of hydrogenation, hydroprocessing, hydrotreating, deoxygenation, isomerization, metathesis, ozonolysis, epoxidation, oxidation, hydrolysis, sulfation, sulfonation, ethoxylation, propoxylation, amidation, or saponification. 
     
     
         16 . The product of  claim 15 , wherein the product is an oleochemical. 
     
     
         17 . The product of  claim 16 , wherein the oleochemical is selected from the group consisting of fatty nitriles, esters, dimer acids, quats, surfactants, fatty alkanolamides, fatty alcohol sulfates, resins, emulsifiers, fatty alcohols, olefins, drilling muds, polyols, polyurethanes, polyacrylates, rubber, candles, cosmetics, metallic soaps, soaps, alpha-sulphonated methyl esters, fatty alcohol sulfates, fatty alcohol ethoxylates, fatty alcohol ether sulfates, imidazolines, surfactants, detergents, esters, quats, ozonolysis products, fatty amines, fatty alkanolamides, ethoxysulfates, monoglycerides, diglycerides, lubricants, hydraulic fluids, greases, dielectric fluids, mold release agents, metal working fluids, heat transfer fluids, functional fluids, industrial chemicals, surface coatings, paints and lacquers, electrical wiring insulation, and waxes. 
     
     
         18 . A food product comprising a triglyceride oil, wherein the fatty acid profile of the triglyceride oil comprises at least 60% C18:1 and less than 7% C18:2. 
     
     
         19 . A food product of  claim 18 , wherein the food product is a frying oil. 
     
     
         20 . A food product of  claim 18 , wherein the food product comprises cocoa butter and/or chocolate.