Methods for recovering peptides/amino acids and oil/fat from one or more protein-containing raw materials, and products produced by the methods

Abstract

According to a first aspect, hydrolysis of a protein-containing raw material and separation of amino acids/peptides is carried out, wherein the hydrolysis is effected by using the endogenous enzymes of the protein-containing raw material. The hydrolysate is passed through a membrane filter, wherein peptide/amino acids follow a permeate stream, whilst the active enzymes continuously break down any protein residues that are deposited on the membrane surface. The enzymes are passed together with retentate back to the hydrolysis. Furthermore, an amino acid and peptide product and an oil product are described and the use thereof is disclosed.

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         27 . A method for producing peptides/amino acids with a fat content of less than 0.5% by weight from a protein-containing raw material, wherein the method comprises:
 (a) grinding the protein-containing raw material;   (b) heating the ground raw material to temperatures in the range of 40-62° C.;   (c) adding water which has approximately the same, or the same, temperature as the raw material, and wherein the pH of the water is adjusted to 7.0-8.5 by adding calcium;   (d) hydrolysing the ground raw material with endogenous enzymes in order to prepare a hydrolysate;   (e) removing solid particles and non-hydrolysed proteins which can be returned to the hydrolysis from the hydrolysate;   (f) periodically or continually separating off fat/oil in order to obtain an oil product;   (g) separating off the desired molecular weight fraction of peptides/amino acids by membrane filtration;   (h) routing the portions of the hydrolysate that do not penetrate the membrane filter in step (g) back to the hydrolysis in step (d);   (i) concentrating and optionally drying the permeate in order to obtain peptides/amino acids; and   (j) wholly or partly returning the distillate from the concentrating to the permeate side of the membrane filter.   
     
     
         28 . The method according to  claim 27 , wherein the method takes place as a closed process. 
     
     
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         30 . The method according to  claim 27 , wherein the method further comprises dividing the solid particles from step (e) into hydroxyapatite, protein residues and other solid particles. 
     
     
         31 . The method according to  claim 27 , wherein the oil product recovered in step (f) is passed through a sterile filter, and any heavy portions are removed in order to obtain a cold-processed, protein-free sterile oil. 
     
     
         32 . A method for the hydrolysis of one or more protein-containing raw materials wherein the hydrolysis is carried out using endogenous enzymes of the one or more protein-containing material or materials, the method comprising:
 heating the one or more protein-containing raw materials;   pumping the one or more protein-containing raw materials into a hydrolysis tank; and   adding water having a temperature of 45-58° C. in an amount of 10-40%,   and wherein the mixture is constantly stirred and pumped, wherein during the hydrolysis the pH is 7.6-8.2.   
     
     
         33 . A method for removing peptides and amino acids from a hydrolysis mixture, wherein the hydrolysis mixture contains active enzymes, amino acids, peptides and non-converted proteins, the method comprising:
 passing the hydrolysis mixture through a membrane filter, wherein amino acids and any peptides having a molecular weight less than 10,000 Daltons are filtered off and the active enzymes break down proteins that are deposited on the membrane filter.   
     
     
         34 . A pharmaceutical product comprising one or more peptides/amino acids produced by the method of  claim 27 . 
     
     
         35 . A biotechnological product comprising one or more peptides/amino acids produced by the method of  claim 27 . 
     
     
         36 . A food product comprising one or more peptides/amino acids produced by the method of  claim 27 . 
     
     
         37 . A feed product comprising one or more peptides/amino acids produced by the method of  claim 27 . 
     
     
         38 . A product containing hydroxyapatite produced by the method of  claim 30 . 
     
     
         39 . Amino acids/peptides produced by the method according to  claim 27 , wherein the amino acids/peptides do not contain allergens and DNA traces, wherein the free amino acid content is 30-60% by weight, the fat content is less than 0.5% by weight and the salt content is less than 1% by weight. 
     
     
         40 . A product comprising sterile oil produced by the method according to  claim 31 , wherein the sterile oil does not contain allergens or DNA traces. 
     
     
         41 . The hydroxyapatite produced by the method according to  claim 30 , wherein the hydroxyapatite does not contain allergens or DNA traces. 
     
     
         42 . Amino acids/peptides produced by the method according to  claim 27 , wherein the amino acids/peptides do not contain allergens and DNA traces, wherein the free amino acid content is 5-100% by weight, the fat content is less than 0.1% by weight and the salt content is less than 0.5% by weight. 
     
     
         43 . The method according to  claim 27 , further comprising, before and/or after step (b):
 separating oil/fat from the raw materials in order to obtain a second oil product.   
     
     
         44 . The method according to  claim 27 , further comprising, before and/or after step (d):
 adding a pH adjuster in order to maintain the desired pH value of 7.0-8.5 of the hydrolysate.   
     
     
         45 . The method according to  claim 27 , further comprising, after step (f):
 treating the hydrolysate against microorganism growth with UV.   
     
     
         46 . The method of  claim 27 , wherein, at step (b), the temperature is in the range 45-58° C. 
     
     
         47 . The method of  claim 27 , wherein the membrane filtration is of crossflow type. 
     
     
         48 . The method of  claim 31 , wherein the heavy portions comprise stearic acid.