Protein fragment complementation assays for the detection of biological or drug interactions

Abstract

The present invention describes a method for detecting biomolecular interactions said method comprising: (a) selecting an appropriate reporter molecule selected from the group consisting of a protein, a fluorescent protein, a luminescent protein and a phosphorescent protein; (b) effecting fragmentation of said reporter molecule such that said fragmentation results in reversible loss of reporter function; (c) fusing or attaching fragments of said reporter molecule separately to other molecules; followed by (d) reassociation of said reporter fragments through interactions of the molecules that are fused to said fragments; and (e) detecting said biomolecular interactions by reconstitution of activity of the reporter molecule

1 - 46 . (canceled) 
     
     
         47 . A method of testing the ability of a compound to inhibit molecular interactions in a PCA comprising performing a PCA in the presence of said compound and correlating any inhibition with said presence. 
     
     
         48 - 58 . (canceled) 
     
     
         59 . A host cell transfected with a nucleic acid composition coding for complementary fragments of a first molecule, each fused to separate molecules