Viral Immunogenic Compositions

Abstract

Disclosed herein are immunogenic compositions for producing immediate and sustained immunity to infectious viral and bacteriological pathogens. A univalent immunogenic composition is disclosed comprising an isolated antigen and a polynucleotide formulated into a nanoparticle or liposome. Furthermore, multivalent immunogenic compositions are disclosed comprising multiple univalent immunogenic compositions. Also disclosed, are methods of inducing protective or therapeutic immune responses in individuals comprising administering one or more univalent immunogenic compositions.

1 . A univalent immunogenic composition comprising an isolated antigen and a polynucleotide formulated into a nanoparticle or liposome. 
     
     
         2 . The univalent immunogenic composition according to  claim 2 , further comprising an adjuvant. 
     
     
         3 . The univalent immunogenic composition according to  claim 2 , wherein the adjuvant is alum. 
     
     
         4 . The univalent immunogenic composition according to  claim 1 , wherein the adjuvant is formulated into the nanoparticle or liposome or admixed with the nanoparticle or liposome formulated univalent immunogenic composition. 
     
     
         5 . The univalent immunogenic composition according to  claim 1 , wherein the isolated antigen is an influenza virus antigen. 
     
     
         6 . The univalent immunogenic composition according to  claim 5 , wherein the influenza virus antigen is a hemagglutinin, a neuraminidase, or a matrix protein. 
     
     
         7 . The univalent immunogenic composition according to  claim 1 , wherein the polynucleotide is a synthetic polynucleotide comprising double stranded DNA or double stranded RNA. 
     
     
         8 . The univalent immunogenic composition according to  claim 7 , wherein the double stranded DNA or double stranded RNA comprises nucleotide repeats. 
     
     
         9 . The univalent immunogenic composition according the  claim 8 , wherein the polynucleotide is poly-IC or poly-ICLC. 
     
     
         10 . The univalent immunogenic composition according to  claim 1  wherein the liposome is a self-assembling liposome system. 
     
     
         11 . The univalent immunogenic composition according to  claim 1 , wherein the liposome further comprises monophosphoryl lipid A or pullulans. 
     
     
         12 . A method of inducing a protective or therapeutic immune response in an individual comprising administering one or more univalent immunogenic compositions according to  claim 1 , wherein a first univalent immunogenic composition comprises a first antigen. 
     
     
         13 . The method according to  claim 12 , wherein a second univalent immunogenic composition comprises a second antigen. 
     
     
         14 . The method according to  claim 12 , wherein the one or more univalent immunogenic compositions are administered by a method selected from the group consisting of scarification, intradermal injection, intramuscular injection, sub-lingual administration, and inhalation. 
     
     
         15 . The method according to  claim 12 , wherein the one or more univalent immunogenic compositions are administered by injection to the individual at separate sites on the individual. 
     
     
         16 . The method according to  claim 12 , wherein the one or more univalent immunogenic compositions are administered at different times. 
     
     
         17 . The method according to  claim 12 , wherein the one or more univalent immunogenic compositions are administered substantially simultaneously. 
     
     
         18 . The method according to  claim 12 , wherein the one or more univalent immunogenic compositions are administered by injection to the individual at the same site on the individual. 
     
     
         19 . The method according to  claim 12 , wherein the one or more univalent immunogenic compositions are combined prior to injection into a multivalent immunogenic composition. 
     
     
         20 . A multivalent immunogenic composition comprising two or more univalent immunogenic compositions according to  claim 1 , wherein each of the univalent immunogenic compositions comprises a different antigen.