Methods for genetic analysis of dna to detect sequence variances

Abstract

Methods for determining genotypes and haplotypes of genes are described. Also described are single nucleotide polymorphisms and haplotypes in the ApoE gene and methods of using that information.

1 . A method of detecting the presence of a variance in a target nucleic acid sequence, comprising
 amplifying a template nucleic acid sequence to generate an amplification product, wherein said amplifying results in the insertion of a bifunctional restriction enzyme recognition site into said amplification product; and   analyzing said amplification product.   
     
     
         2 - 10 . (canceled)