Method for Measuring Low-Density Lipoprotein (LDL) Cholesterol and Test Piece for Measuring LDL Cholesterol

Abstract

An LDL cholesterol measurement method that can be used with a test piece is provided. The LDL cholesterol measurement method for measuring LDL cholesterol in a sample has (A) a step of providing a total-cholesterol measurement portion and a non-LDL cholesterol measurement portion for measuring non-LDL cholesterol, which is cholesterol other than LDL cholesterol; (B) a step of measuring the total cholesterol in the sample in the total-cholesterol measurement portion; (C) a step of measuring the non-LDL cholesterol in the sample in the non-LDL cholesterol measurement portion; and (D) a step of obtaining the LDL cholesterol level in the sample by subtracting the non-LDL cholesterol value measured in the step (C) from the total-cholesterol value measured in the step (B).

1 - 9 . (canceled) 
     
     
         10 . A test piece for use in a method for measuring low-density lipoprotein (LDL) cholesterol in a sample, the test piece comprising
 a total cholesterol measurement portion; and   a non-LDL cholesterol measurement portion for measuring a non-LDL cholesterol level, which is a level of cholesterol other than LDL cholesterol,   wherein the method comprises
 (B) a step of measuring total cholesterol in the sample in the total-cholesterol measurement portion; 
 (C) a step of measuring non-LDL cholesterol in the same in the non-LDL cholesterol measurement portion; and 
 (D) a step of obtaining an LDL cholesterol level in the sample by subtracting a non-LDL cholesterol value measured in the step (C) from a total cholesterol value measured in the step (B), 
   wherein the total-cholesterol measurement portion comprises an enzyme reagent for measuring cholesterol and the non-LDL cholesterol and   wherein the non-LDL cholesterol measurement portion comprises an enzyme reagent for measuring cholesterol, a surfactant for solubilizing non-LDL cholesterol and a surfactant for inhibiting LDL cholesterol dissolution.   
     
     
         11 . The test piece for measuring LDL cholesterol according to  claim 10 , wherein the non-LDL cholesterol measurement portion further comprises an LDL cholesterol agglomerating agent. 
     
     
         12 . The test piece for measuring LDL cholesterol according to  claim 10 , wherein the surfactant for solubilizing non-LDL cholesterol comprises at least one surfactant selected from polyoxyethylene alkylene phenyl ethers and polyoxyethylene alkylene tribenzyl phenyl ethers; and
 the surfactant for inhibiting LDL cholesterol dissolution comprises at least one surfactant selected from the group consisting of polyoxyethylene alkyl ethers, polyoxyethylene alkyl phenyl ethers, polyoxyethylene-polyoxypropylene condensation products, polyoxyethylene alkyl ether sulfate salts and alkylbenzene sulfonate salts.   
     
     
         13 . The test piece for measuring LDL cholesterol according to  claim 10 , wherein the enzyme reagent comprises a cholesterol esterase and a cholesterol oxidase. 
     
     
         14 . The test piece for measuring LDL cholesterol according to  claim 10 , wherein the enzyme reagent comprises a cholesterol esterase and a cholesterol dehydrogenase. 
     
     
         15 . The test piece for measuring LDL cholesterol according to  claim 11 , wherein the LDL cholesterol agglomerating agent is a polyvinyl sulfate salt. 
     
     
         16 . The test piece for measuring LDL cholesterol according to  claim 10 , wherein the non-LDL cholesterol is high-density lipoprotein (HDL) cholesterol and very-low-density lipoprotein (VLDL) cholesterol. 
     
     
         17 . (canceled) 
     
     
         18 . The test piece for measuring LDL cholesterol according to  claim 10  comprising a first support, a second support, a first reagent layer and a second reagent layer,
 the first reagent layer being disposed on the first support, 
 the second reagent layer being disposed on the second support, 
 the first reagent layer serving as the total-cholesterol measurement portion, 
 the second reagent layer serving as the non-LDL cholesterol measurement portion.