US2013040383A1PendingUtilityA1

Nucleic acid molecules encoding anti-inflammatory domain antibodies

Assignee: CEPHALON AUSTRALIA PTY LTDPriority: Dec 20, 2005Filed: Aug 14, 2012Published: Feb 14, 2013
Est. expiryDec 20, 2025(expired)· nominal 20-yr term from priority
A61P 9/10A61P 37/06A61P 9/00A61P 37/00A61P 35/04A61P 31/04A61P 29/00A61P 35/00A61P 3/10A61P 25/00A61P 31/00A61P 21/00A61P 19/00A61P 19/10C07K 16/241A61P 17/02A61P 19/02A61P 11/00A61P 1/16A61P 1/04A61P 17/00C07K 2317/569A61P 1/00C07K 2317/24C07K 2317/565C07K 16/24C07K 16/00A61K 39/395
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Claims

Abstract

The present invention provides a recombinant domain antibody (dAb) which binds to human TNF-α, the dAb comprising an immunoglobulin heavy or light chain variable domain, comprising at least one complementarity determining region (CDR) having a sequence derived from a New World primate.

Claims

exact text as granted — not AI-modified
1 . (canceled) 
     
     
         2 . An isolated nucleic acid molecule encoding a domain antibody (dAb) which binds to human TNF-α, wherein the dAb comprises an amino acid sequence selected from the group consisting of: 
       
         
           
                 
               
                   (SEQ ID NO: 8) 
                 
                   DIQMTQSPSSLSASVGDRVTITCRASQAIDSYLHWYQQKPGKAPKLLIYS 
                 
                     
                 
                   ASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQVVWRPFTFGQ 
                 
                     
                 
                   GTKVEIKR; 
                 
                     
                 
                   (SEQ ID NO: 9) 
                 
                   DIQMTQSPSSLSASVGDRVTITCRASQSIDSYLHWYQQKPGKAPKLLIYS 
                 
                     
                 
                   ASNLETGVPSRFSGSGSGTDFTLTISSLLPEDFATYYCQQVVWRPFTFGQ 
                 
                     
                 
                   GTKVEIKR; 
                 
                     
                 
                   (SEQ ID NO: 10) 
                 
                   DIQMTQSPSSLSASVGDRVTITCRASQAIDSYLHWYQQKPGKAPKLLIYS 
                 
                     
                 
                   ASNLETGVPSRFSGSGSGTDFTLTISSLLPEDFATYYCQQVVWRPFTFGQ 
                 
                     
                 
                   GTKVEIKR; 
                 
                     
                 
                   (SEQ ID NO: 50) 
                 
                   DIQMTQSPSSLSASVGDRVTITCRASQSIDSYLHWYQQKPGKPPKLLIYS 
                 
                     
                 
                   ASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQVVWRPFTFGQ 
                 
                     
                 
                   GTKVEIKR; 
                 
                     
                 
                   (SEQ ID NO: 51) 
                 
                   DIQMTQSPSSLSASVGDRVTITCRASQSIDSYLHWYQQKPGKAPKLLIYS 
                 
                     
                 
                   ASNLETGVPSRFSGRGSGTDFTLTISSLQPEDFATYYCQQVVWRPFTFGQ 
                 
                     
                 
                   GTKVEIKR; 
                 
                   and 
                 
                     
                 
                   (SEQ ID NO: 52) 
                 
                   DIQMTQSPSSLSASVGDRVTITCRASQSIDSYLHWYQQKPGKAPKLLIYS 
                 
                     
                 
                   ASNLETGVPSRFSGSGSGTDFTLTISSLLPEDFATYYCQQVVWRPFTFGQ 
                 
                     
                 
                   GTKVEIKR. 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         3 . The isolated nucleic acid molecule of  claim 2 , wherein the dAb comprises the amino acid sequence: 
       
         
           
                 
               
                   (SEQ ID NO: 10) 
                 
                   DIQMTQSPSSLSASVGDRVTITCRASQAIDSYLHWYQQKPGKAPKLLIYS 
                 
                     
                 
                   ASNLETGVPSRFSGSGSGTDFTLTISSLLPEDFATYYCQQVVWRPFTFGQ 
                 
                     
                 
                   GTKVEIKR. 
                 
             
                
                
                
                
                
                
               
            
           
         
       
     
     
         4 . An expression vector comprising the nucleic acid molecule of  claim 2 . 
     
     
         5 . An expression vector comprising the nucleic acid molecule of  claim 3 . 
     
     
         6 . An isolated nucleic acid molecule encoding a domain antibody (dAb) which binds to human TNF-α, wherein the dAb consists of an amino acid sequence selected from the group consisting of: 
       
         
           
                 
               
                   (SEQ ID NO: 8) 
                 
                   DIQMTQSPSSLSASVGDRVTITCRASQAIDSYLHWYQQKPGKAPKLLIYS 
                 
                     
                 
                   ASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQVVWRPFTFGQ 
                 
                     
                 
                   GTKVEIKR; 
                 
                     
                 
                   (SEQ ID NO: 9) 
                 
                   DIQMTQSPSSLSASVGDRVTITCRASQSIDSYLHWYQQKPGKAPKLLIYS 
                 
                     
                 
                   ASNLETGVPSRFSGSGSGTDFTLTISSLLPEDFATYYCQQVVWRPFTFGQ 
                 
                     
                 
                   GTKVEIKR; 
                 
                     
                 
                   (SEQ ID NO: 10) 
                 
                   DIQMTQSPSSLSASVGDRVTITCRASQAIDSYLHWYQQKPGKAPKLLIYS 
                 
                     
                 
                   ASNLETGVPSRFSGSGSGTDFTLTISSLLPEDFATYYCQQVVWRPFTFGQ 
                 
                     
                 
                   GTKVEIKR; 
                 
                     
                 
                   (SEQ ID NO: 50) 
                 
                   DIQMTQSPSSLSASVGDRVTITCRASQSIDSYLHWYQQKPGKPPKLLIYS 
                 
                     
                 
                   ASNLETGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQVVWRPFTFGQ 
                 
                     
                 
                   GTKVEIKR; 
                 
                     
                 
                   (SEQ ID NO: 51) 
                 
                   DIQMTQSPSSLSASVGDRVTITCRASQSIDSYLHWYQQKPGKAPKLLIYS 
                 
                     
                 
                   ASNLETGVPSRFSGRGSGTDFTLTISSLQPEDFATYYCQQVVWRPFTFGQ 
                 
                     
                 
                   GTKVEIKR; 
                 
                   and 
                 
                     
                 
                   (SEQ ID NO: 52) 
                 
                   DIQMTQSPSSLSASVGDRVTITCRASQSIDSYLHWYQQKPGKAPKLLIYS 
                 
                     
                 
                   ASNLETGVPSRFSGSGSGTDFTLTISSLLPEDFATYYCQQVVWRPFTFGQ 
                 
                     
                 
                   GTKVEIKR. 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         7 . The isolated nucleic acid molecule of  claim 6 , wherein the dAb consists of the amino acid sequence: 
       
         
           
                 
               
                   (SEQ ID NO: 10) 
                 
                   DIQMTQSPSSLSASVGDRVTITCRASQAIDSYLHWYQQKPGKAPKLLIYS 
                 
                     
                 
                   ASNLETGVPSRFSGSGSGTDFTLTISSLLPEDFATYYCQQVVWRPFTFGQ 
                 
                     
                 
                   GTKVEIKR. 
                 
             
                
                
                
                
                
                
               
            
           
         
       
     
     
         8 . An expression vector comprising the nucleic acid molecule of  claim 6 . 
     
     
         9 . An expression vector comprising the nucleic acid molecule of  claim 7 .

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