Dry powder cells and cell culture reagents and methods of production thereof

Abstract

The present invention relates generally to nutritive medium, medium supplement, media subgroup and buffer formulations. Specifically, powdered nutritive medium, supplement, subgroup formulations, cell culture media comprising all of the necessary nutritive factors for in vitro cell cultivation, buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent are provided. Particularly, methods of production of these media, supplement, subgroup, buffer formulations and kits, and methods for the cultivation of prokaryotic and eukaryotic cells using these dry powdered nutritive media, supplement, subgroup and buffer formulations are provided. Methods of producing sterile, powdered media or supplement (e.g., powdered FBS, powdered transferrin, powdered insulin, powdered organ extracts, powdered growth factors), media subgroup and buffer formulations by gamma irradiation are provided. Methods for producing dry cell powders, comprising spray-drying a cell suspension, and cells, media, media supplement, media subgroup and buffer powders produced by these methods are provided.

1 - 9 . (canceled) 
     
     
         92 . An agglomerated eukaryotic cell culture medium, wherein the medium is a complete media, a media subgroup, a supplement, or a buffer and wherein said medium has been sterilized using gamma irradiation. 
     
     
         93 . The medium of  claim 92 , wherein the medium has been sterilized after packaging. 
     
     
         94 . The medium of  claim 92  that is capable of culturing a eukaryotic cell after reconstitution with a solvent. 
     
     
         95 . The medium of  claim 94 , and a eukaryotic cell. 
     
     
         96 . The medium of  claim 95  wherein said cell is either a plant, animal or a yeast cell. 
     
     
         97 . The medium of  claim 96 , wherein the animal cell is selected from the group consisting of CHO, 293, VERO, BHK, SP2/0, MDBK, COS, AE-1, L5.1, PER-C6, hybridoma cells and HeLa cells. 
     
     
         98 . The medium of  claim 92  wherein the gamma irradiation is done at least 25 kGy for several days. 
     
     
         99 . The medium of  claim 94  wherein the solvent is water. 
     
     
         100 . The medium of  claim 94  wherein the solvent is an aqueous buffer. 
     
     
         101 . The medium of  claim 98  wherein the gamma irradiation inactivates bacteria, fungi, spores and viruses present in the medium, supplement or buffer. 
     
     
         102 . A method of making the medium of  claim 92 , comprising subjecting an agglomerated eukaryotic medium to gamma irradiation. 
     
     
         103 . The method of  claim 102  wherein the inactivation renders the bacteria, fungi, spores and virus incapable of replication. 
     
     
         104 . The method of  claim 103  wherein the gamma-irradiation is done at a total dosage of about 10-100 kGy. 
     
     
         105 . The method of  claim 104  wherein the total dosage is about 15-75 kGy. 
     
     
         106 . The method of  claim 104  wherein the total dosage is about 20-40 kGy. 
     
     
         107 . The method of  claim 104  wherein the gamma-irradiation is done for about 1 hour to about 7 days, or for about 1 hour to about 5 days, or for about 1 hour to about 3 days, or for about 1 hour to about 24 hours.