Suspension Container For Binding Particles For The Isolation Of Biological Material

Abstract

A device, method and system is provided for binding particles for the separation and/or isolation of biological materials. In particular, a container is provided including a suspension of binding particles for the isolation of biological material, inner walls forming an elongate groove at the bottom of said container, and a cover having openings and/or being penetrable for a linear arrangement of multiple pipets or pipet tips, said openings being located above and parallel to said elongate groove.

1 . A suspension container, comprising:
 a suspension of binding particles for the isolation of biological material;   inner walls forming an elongate groove at the bottom of said container; and   a cover having openings and/or being penetrable for a linear arrangement of multiple pipets or pipet tips, said openings being located above and parallel to said elongate groove.   
     
     
         2 . The suspension container of  claim 1 , wherein said inner walls are inclined in an angle to each other and thereby form said elongate groove. 
     
     
         3 . The suspension container of  claim 1 , wherein said binding particles comprise nucleic acid binding particles. 
     
     
         4 . The suspension container of  claim 1 , wherein said binding particles comprise magnetic glass particles. 
     
     
         5 . The suspension container of  claim 1 , further comprising one or more elements selected from the group of:
 fill-openings for filling the suspension of binding particles into the container; and   a removable foil sealing the cover.   
     
     
         6 . The suspension container of  claim 1 , wherein said cover has openings, said openings being covered by a penetrable septum. 
     
     
         7 . The suspension container of  claim 1 , wherein said container is adapted to hold a volume of at least 100 ml. 
     
     
         8 . The suspension container of  claim 1 , wherein said container comprises one or more fastenings for reversibly fixing the container to a shaker. 
     
     
         9 . The suspension container of  claim 1 , wherein the footprint of said container is oval-shaped. 
     
     
         10 . A method for pipetting a suspension of binding particles for the isolation of biological material, said method comprising the automated steps of:
 agitating a container containing said suspension of binding particles, said container comprising inner walls forming an elongate groove at the bottom of said container, said container further comprising a cover having openings and/or being penetrable for a linear arrangement of multiple pipets or pipet tips;   introducing into said suspension said linear arrangement of multiple pipets or pipet tips through said openings and/or penetrable cover; and   aspirating at least a portion of said suspension with a linear arrangement of multiple pipets or pipet tips and releasing the aspirated suspension or a portion thereof into multiple vessels.   
     
     
         11 . The method of  claim 10 , wherein in said linear arrangement of multiple pipets or pipet tips simultaneously releases said suspension into said multiple vessels. 
     
     
         12 . The method of  claim 11 , wherein in said linear arrangement of multiple pipets or pipet tips individually releases said suspension into said multiple vessels by independently manipulating one or more of said multiple pipets or pipet tips of said linear arrangement. 
     
     
         13 . Analytical system for providing binding particles for the isolation of biological material, said analytical system comprising:
 a linear arrangement of multiple pipets or pipet tips;   a container containing a suspension of binding particles for binding said biological material, said container comprising an elongate cavity at its bottom, said container further comprising a cover, said cover having openings and/or being penetrable for said linear arrangement of multiple pipets or pipet tips; and   a shaker for agitating said container and suspending said binding particles.   
     
     
         14 . The analytical system of  claim 13 , wherein the system further comprises a plurality of vessels for receiving said suspension of binding particles from said linear arrangement of multiple pipets or pipet tips. 
     
     
         15 . The analytical system of any of  claim 13 , said system further comprising a separation module for the isolation of said biological material with said binding particles. 
     
     
         16 . The analytical system of  claim 13 , said system further comprising one or more elements selected from the group consisting of:
 a reaction module containing the components of a chemical and/or biochemical reaction;   a detection module for detecting signals evoked by an analyte;   a storage module for reagents and/or disposables; and   a control unit for controlling system components.