Novel soil microorganism, novel oxidoreductase separated from the soil microorganism, gene encoding the oxidoreductase, and method for producing aglycones using the microorganism, the oxidoreductase and the gene

Abstract

The present invention relates to the novel Rhizobium sp. GIN611 KCTC11708BP or to cell extracts thereof, to a novel oxidoreductase which exhibits a glycolytic activity, to a gene encoding the oxidoreductase, to a recombinant strain comprising recombinant vector proteins or to an expression vector encoding recombinant proteins, and to a method for the glycolysis of natural products using same as a biocatalyst. The present invention also relates to a method for producing aglycones from a variety of natural products using same. The novel oxidoreductase separated from the novel microorganism of the present invention does not belong to a glucosidase group but belongs to an oxidoreductase group, and has a glycolytic activity for natural products. The novel oxidoreductase oxidizes the sugar in the aglycones of natural products, thereby producing a variety of aglycones.

1 - 28 . (canceled) 
     
     
         29 .  Rhizobium  sp. having a capability to produce an oxidoreductase comprising an amino acid sequence of SEQ ID NO 3. 
     
     
         30 . The  Rhizobium  sp. according to  claim 29 , which is the  Rhizobium  sp. GIN611. 
     
     
         31 . The  Rhizobium  sp. according to  claim 29 , which is the  Rhizobium  sp. GIN611 KCTC 11708BP. 
     
     
         32 . A cell extract of the  Rhizobium  sp. having a capability to produce an oxidoreductase comprising an amino acid sequence of SEQ ID NO 3. 
     
     
         33 . The cell extract according to  claim 32 , wherein the  Rhizobium  sp. is  Rhizobium  sp. GIN611. 
     
     
         34 . The cell extract according to  claim 32 , wherein the  Rhizobium  sp. is  Rhizobium  sp. GIN611 KCTC 11708BP. 
     
     
         35 . A method for deglycosylating a natural product using  Rhizobium  sp. having a capability to produce an oxidoreductase comprising an amino acid sequence of SEQ ID NO 3, or a cell extract of the  Rhizobium  sp. as a biocatalyst. 
     
     
         36 . The method for deglycosylating a natural product according to  claim 35 , wherein the  Rhizobium  sp. is  Rhizobium  sp. GIN611. 
     
     
         37 . The method for deglycosylating a natural product according to  claim 35 , wherein the  Rhizobium  sp. is  Rhizobium  sp. GIN611 KCTC 11708BP. 
     
     
         38 . The method for deglycosylating a natural product according to  claim 35 , wherein the natural product is a ginsenoside glycoside, an isoflavone glycoside or a flavonoid glycoside. 
     
     
         39 . The method for deglycosylating a natural product according to  claim 35 , wherein the natural product is selected from a group consisting of ginsenoside compound K (CK), ginsenoside Rh2, ginsenoside F2, ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rc, ginsenoside Rb3, ginsenoside F1, ginsenoside Re, daidzin, icariin, camelliaside A and camelliaside B. 
     
     
         40 . The method for deglycosylating a natural product according to  claim 35 , wherein a sugar of the deglycosylated natural product is selected from a group consisting of glucose, galactose, rhamnose, arabinose and xylose and the sugar is degraded by oxidation. 
     
     
         41 . The method for deglycosylating a natural product according to  claim 40 , wherein the glucose is degraded by oxidizing the 3-hydroxyl (OH) group of a glucose residue. 
     
     
         42 . An oxidoreductase comprising an amino acid sequence of SEQ ID NO 3.