Biomarker specific to brain/nerve or specific to neuronal differentiation

Abstract

The invention provides a novel polypeptide and a specific partial peptide thereof, as well as a novel polynucleotide and a specific partial nucleotide thereof, that can be used as a biomarker specific for the brain/nerves or specific for nerve differentiation; an expression vector for such a polynucleotide and a specific partial peptide thereof; a transformant incorporating such an expression vector; an antisense molecule, RNAi-inducing nucleic acid (e.g., siRNA), aptamer, or antibody for such a biomarker, and a composition comprising the same; a mammalian cell or non-human mammal wherein the expression or a function of such a biomarker is regulated; a measuring means (e.g., primer set, nucleic acid probe, antibody, aptamer) for such a biomarker, and a reagent comprising the same and the like.

1 .- 27 . (canceled) 
     
     
         28 . An isolated peptide comprising the amino acid sequence of SEQ ID NO: 60. 
     
     
         29 . A polynucleotide that encodes the peptide of  claim 28 . 
     
     
         30 . An expression vector comprising the polynucleotide of  claim 29  and a promoter operably linked thereto. 
     
     
         31 . A transformant incorporating the expression vector of  claim 30 . 
     
     
         32 . An aptamer that binds the peptide of  claim 28 . 
     
     
         33 . An antibody that binds the peptide of  claim 28 . 
     
     
         34 . The antibody of  claim 33 , wherein the antibody is any one of the (i) to (iii) below:
 (i) a polyclonal antibody;   (ii) a monoclonal antibody or a portion thereof;   (iii) a chimeric antibody, a humanized antibody or a human antibody.   
     
     
         35 . A cell that produces the antibody of  claim 33 . 
     
     
         36 . The cell of  claim 35 , wherein the cell is a hybridoma. 
     
     
         37 . A composition comprising (a) the antibody of  claim 33 , or an expression vector therefor, and (b) a pharmaceutically acceptable carrier. 
     
     
         38 . A reagent or kit for detection or quantification of any one of the polypeptides encoded by the brain/nerve-specific genes 1 to 10, which reagent or kit comprises one or more antibodies of  claim 33 . 
     
     
         39 . The peptide of  claim 28  consisting of the amino acid sequence of SEQ ID NO: 60. 
     
     
         40 . A polynucleotide that encodes the peptide of  claim 39 . 
     
     
         41 . An expression vector comprising the polynucleotide of  claim 40  and a promoter operably linked thereto. 
     
     
         42 . A transformant incorporating the expression vector of  claim 41 . 
     
     
         43 . An aptamer that binds the peptide of  claim 39 . 
     
     
         44 . An antibody that binds the peptide of  claim 39 . 
     
     
         45 . The antibody of  claim 44 , wherein the antibody is any one of the (i) to (iii) below:
 (i) a polyclonal antibody;   (ii) a monoclonal antibody or a portion thereof;   (iii) a chimeric antibody, a humanized antibody or a human antibody.   
     
     
         46 . A cell that produces the antibody of  claim 44 . 
     
     
         47 . The cell of  claim 46 , wherein the cell is a hybridoma. 
     
     
         48 . A composition comprising (a) the antibody of  claim 44 , or an expression vector therefor, and (b) a pharmaceutically acceptable carrier. 
     
     
         49 . A reagent or kit for detection or quantification of any one of the polypeptides encoded by the brain/nerve-specific genes 1 to 10, which reagent or kit comprise one or more antibodies of  claim 44 .