US2013096300A1PendingUtilityA1

Long wavelength fluorogenic intracellular ion indicators that are well retained in the cytosol

Assignee: GEE KYLEPriority: Jun 5, 2007Filed: Sep 12, 2012Published: Apr 18, 2013
Est. expiryJun 5, 2027(~0.9 yrs left)· nominal 20-yr term from priority
C07D 311/90C09B 11/22C07D 491/04C07D 413/10G01N 33/582A61K 31/353C07D 413/04C07D 491/147G01N 33/84G01N 33/6872C09B 11/24G01N 2223/406G01N 2458/00
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Claims

Abstract

Cell permeable metal ion indicator compounds and methods of their use and synthesis are described. The compound comprises a metal chelating moiety (M c ), a reporter molecule and two or more lipophilic groups (G L ) covalently bonded through a linker to the reporter molecule, wherein the lipophilic groups, when present in a live cell, are cleaved resulting in two or more negatively charged groups.

Claims

exact text as granted — not AI-modified
1 . An intracellular ion indicator compound, wherein the compound comprises a metal chelating moiety (M C ), a reporter molecule and two or more lipophilic groups (G L ) covalently bonded through a linker to the reporter molecule, wherein the lipophilic groups, when present in a live cell, are cleaved resulting in two or more negatively charged groups. 
     
     
         2 - 45 . (canceled)

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