US2013101995A1PendingUtilityA1

Device and method for pressure-driven plug transport

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Assignee: RUSTEM ISMAGILOV FPriority: May 9, 2002Filed: Oct 10, 2012Published: Apr 25, 2013
Est. expiryMay 9, 2022(expired)· nominal 20-yr term from priority
Inventors:Ismagilov F. RustemJoshua David TiceCory John GerdtsBo Zheng
B01J 2219/00599B01D 9/0072B82Y 30/00B01J 19/0046C12N 9/2462B01L 3/502784Y10T436/143333B01J 2219/00889B01J 2219/00869B01L 3/502715B01J 2219/00722B01J 2219/00756B01J 2219/00576B01J 2219/00903C07K 14/43B01J 2219/00837Y10T137/0318B01J 2219/00736B01J 2219/00977G01N 35/08Y10T436/25G01N 1/28Y10T117/10B01J 2219/00975B01L 2200/0673B01J 2219/00585B01J 2219/00891B01J 2219/00725B01J 2219/00783B01D 2009/0086C30B 29/54B01J 2219/00286B01J 19/0093C12Q 1/6806B01J 14/00Y10T436/117497B01L 2400/0487B01L 2300/0867C12Y 302/01017B01J 2219/0086C30B 7/14C30B 29/58B01J 2219/00894B01L 2200/12B01L 3/50273B01F 25/433B01F 25/4331B01F 33/3021
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Claims

Abstract

The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.

Claims

exact text as granted — not AI-modified
1 - 20 . (canceled) 
     
     
         21 . A method of forming a plug, the method comprising:
 forming a discrete plug of aqueous fluid by partitioning with co-flowing streams of oil the aqueous fluid as it continuously flows through a microfluidic channel, the plug comprising at least one DNA or RNA molecule.   
     
     
         22 . The method of  claim 21 , wherein the plug is surrounded by oil. 
     
     
         23 . The method of  claim 22 , wherein the oil comprises a surfactant. 
     
     
         24 . The method of  claim 23 , wherein the surfactant is a fluorosurfactant. 
     
     
         25 . The method of  claim 22 , wherein the oil is fluorinated. 
     
     
         26 . The method of  claim 21 , further comprising the step of conducting a reaction in the plug. 
     
     
         27 . The method of  claim 26 , wherein the reaction is an autocatalytic reaction. 
     
     
         28 . The method of  claim 27 , further comprising the step of detecting a product of the autocatalytic reaction. 
     
     
         29 . The method of  claim 28 , wherein the detecting step comprises optically detecting the product.

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