Isolated eIF-5A and Polynucleotides Encoding Same

Abstract

The present invention relates to unique isoforms of eukaryotic initiation Factor 5A (“eIF-5A”): senescence-induced eIF-5A; wounding-induced eIF-5A; and growth eIF-5A, as well as polynucleotides that encode these three factors. The present invention also relates to methods involving modulating the expression of these factors. The present invention also relates to deoxyhypusine synthase (“DHS”), polynucleotides that encode DHS, and methods involving modulating the expression of DHS.

1 - 25 . (canceled) 
     
     
         26 : An isolated growth eIF-5A polypeptide with at least 95% sequence identity to SEQ ID NO: 67. 
     
     
         27 : The isolated polypeptide of  claim 26 , wherein the polypeptide comprises SEQ ID NO: 67. 
     
     
         28 : The isolated polypeptide of  claim 26 , wherein the polypeptide consists of SEQ ID NO: 67. 
     
     
         29 : The isolated polypeptide of  claim 26 , wherein the growth eIF-5A polypeptide is from canola. 
     
     
         30 : An isolated polynucleotide comprising a nucleotide sequence encoding the polypeptide of  claim 26 . 
     
     
         31 : The polynucleotide of  claim 30  wherein the polynucleotide sequence comprises SEQ ID NO: 66. 
     
     
         32 : The polynucleotide of  claim 30  wherein the polynucleotide sequence consists of SEQ ID NO: 66. 
     
     
         33 : A vector comprising the polynucleotide of  claim 31  or  32  and regulatory sequences operatively linked to the polynucleotide to provide transcription of the polynucleotide in a plant. 
     
     
         34 : The vector of  claim 33 , wherein the plant is canola. 
     
     
         35 : A bacterial cell transformed with the vector according to  claim 33 . 
     
     
         36 : A plant cell transformed with the vector according to  claim 33 . 
     
     
         37 : A plant and progeny thereof, wherein the plant is generated from the plant cell of  claim 36  and wherein said plant and progeny thereof comprise said vector. 
     
     
         38 : A method of increasing expression of endogenous growth eIF-5A in a plant comprising incorporating into the genome of at least one plant cell the vector of  claim 33 , whereby transcription of the polynucleotide results in increase of expression of growth eIF-5A in the plant. 
     
     
         39 : A method of  claim 38  wherein the plant is canola. 
     
     
         40 : A plant produced by the method of  claim 38 . 
     
     
         41 : Progeny, plant parts or seeds of the plant of  claim 40 .