Process for the modulation of the antagonistic activity of a monoclonal antibody

Abstract

The present disclosure relates to the antibody engineering field and, more particularly, to a process for the screening of antibodies and/or the modulation of the agonistic/antagonistic activity of antibodies. More particularly, the disclosure concerns a process of improving the antagonistic activity of a monoclonal antibody directed against a specific target molecule, or a divalent functional fragment or derivative thereof, said antibody being capable of inhibiting one or more of the biological activities of said target molecule, wherein said process comprises a stage of reconfiguration of the image region consisting of a modification of the amino acid sequence of said hinge region by the deletion, the addition or the substitution of at least one amino aced. The disclosure also relates to polypeptides useful for such a modulation method and the obtained antibodies.

1 . A process of improving the antagonistic activity of a monoclonal antibody comprising a hinge region, or of a divalent functional fragment or derivative of said antibody, wherein said process comprises modifying the amino acid sequence of said hinge region of said antibody by the deletion, the addition, or the substitution of at least one amino acid of said hinge region, thereby improving the antagonistic activity of said antibody;
 wherein the antagonistic activity of said antibody comprises being capable of inhibiting one or more biological activities of a target molecule.   
     
     
         2 .- 30 . (canceled)