Method for detecting and quantifying endogenous wheat dna sequence

Abstract

A primer pair is provided capable of amplifying partial sequences of endogenous wheat DNA which are single copies and which allow wheat to be specifically detected without cross-reacting with other plants in a polymerase chain reaction. Also provided is a kit for detecting or assaying an endogenous wheat DNA sequence in a test sample by a polymerase chain reaction.

1 - 13 . (canceled) 
     
     
         14 . A primer pair for detecting or quantifying wheat in a test sample by PCR, wherein said primer pair is capable of amplifying a region comprising at least 80% of a nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 2. 
     
     
         15 . The primer pair according to claim  1 , selected from the group consisting of
 (i) a primer pair comprising a first primer comprising the nucleotide sequence of SEQ ID NO: 8 and a second primer comprising the nucleotide sequence of SEQ ID NO: 9,   (ii) a primer pair comprising a first primer comprising the nucleotide sequence of SEQ ID NO: 10 and a second primer comprising the nucleotide sequence of SEQ ID NO: 11, and   (vii) primer pairs consisting of pairs of nucleic acids each comprising the continuous sequence of at least 80% of the nucleotide sequence of a nucleic acid in one of the primer pairs (i)-(ii).   
     
     
         16 . A kit for detecting or assaying an endogenous wheat DNA sequence in a test sample by PCR, comprising a primer pair according to claim  1 . 
     
     
         17 . A kit for detecting or assaying an endogenous wheat DNA sequence in a test sample by PCR, comprising a primer pair according to claim  2 .