Promoter, promoter control elements, and combinations, and uses thereof

Abstract

The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels.

1 . An isolated nucleic acid molecule capable of modulating transcription wherein the nucleic acid molecule shows at least 80% sequence identity to any one of the promoter sequences in the Sequence Listing, or a complement thereof. 
     
     
         2 . The isolated nucleic acid molecule of  claim 1 , wherein said nucleic acid is capable of functioning as a promoter. 
     
     
         3 . The isolated nucleic acid molecule of  claim 2 , wherein said nucleic acid comprises a reduced promoter nucleotide sequence having a sequence consisting of one of the promoter sequences in Table 1 having at least one of the corresponding optional promoter fragments identified in Table 1. 
     
     
         4 . The isolated nucleic acid molecule of  claim 2 , wherein said nucleic acid comprises a reduced promoter nucleotide sequence having a sequence consisting of one of the promoter sequences in Table 1 having all of the corresponding optional promoter fragments identified in Table 1. 
     
     
         5 . The isolated nucleic acid molecule of  claim 1 , wherein said nucleic acid molecule is capable of modulating transcription during the developmental times, or in response to a stimulus, or in a cell, tissue, or organ as set forth in Table 1 in the section “The spatial expression of the promoter-marker-vector.” 
     
     
         6 . A vector construct comprising:
 a) a first nucleic acid capable of modulating transcription wherein the nucleic acid molecule shows at least 80% sequence identity to any one of the promoter sequences in the Sequence Listing; and   b) a second nucleic acid having to be transcribed,   
       wherein said first and second nucleic acid molecules are heterologous to each other and are operably linked together. 
     
     
         7 . The vector construct according to  claim 6 , wherein said nucleic acid comprises a reduced promoter nucleotide sequence having a sequence consisting of one of the promoter sequences in Table 1 having at least one of the corresponding optional promoter fragments identified in Table 1 deleted therefrom. 
     
     
         8 . The vector construct according to  claim 6 , wherein said nucleic acid comprises a reduced promoter nucleotide sequence having a sequence consisting of one of the promoter sequences in Table 1 having all of the corresponding optional promoter fragments identified in Table 1 deleted therefrom. 
     
     
         9 . A host cell comprising an isolated nucleic acid molecule according to  claim 1 , wherein said nucleic acid molecule is flanked by exogenous sequence. 
     
     
         10 . The host cell according to  claim 9 , wherein said nucleic acid comprises a reduced promoter nucleotide sequence having a sequence consisting of one of the promoter sequences in Table 1 having at least one of the corresponding optional promoter fragments identified in Table 1 deleted therefrom. 
     
     
         11 . The host cell according to  claim 9 , wherein said nucleic acid comprises a reduced promoter nucleotide sequence having a sequence consisting of one of the promoter sequences in Table 1 having all of the corresponding optional promoter fragments identified in Table 1 deleted therefrom. 
     
     
         12 . A host cell comprising a vector construct of  claim 6 . 
     
     
         13 . A method of modulating transcription by combining, in an environment suitable for transcription:
 a) a first nucleic acid molecule capable of modulating transcription wherein the nucleic acid molecule shows at least 80% sequence identity to any one of the promoter sequences in the Sequence Listing; and   b) a second molecule to be transcribed;   
       wherein the first and second nucleic acid molecules are heterologous to each other and operably linked together. 
     
     
         14 . The method of  claim 13 , wherein said nucleic acid comprises a reduced promoter nucleotide sequence having a sequence consisting of one of the promoter sequences in Table 1 having at least one of the corresponding optional promoter fragments identified in Table 1 deleted therefrom. 
     
     
         15 . The method of  claim 13 , wherein said nucleic acid comprises a reduced promoter nucleotide sequence having a sequence consisting of one of the promoter sequences in Table 1 having all of the corresponding optional promoter fragments identified in Table 1 deleted therefrom. 
     
     
         16 . The method according to any one of  claims 13 - 15 , wherein said first nucleic acid molecule is capable of modulating transcription during the developmental times, or in response to a stimuli, or in a cell tissue, or organ as set forth in Table 1 in the section entitled “The spatial expression of the promoter-marker-vector”wherein said first nucleic acid molecule is inserted into a plant cell and said plant cell is regenerated into a plant. 
     
     
         17 . A plant comprising a vector construct according to  claim 6 .