US2013122569A1PendingUtilityA1
Expression of Granular Starch Hydrolyzing Enzymes in Trichoderma and Process for Producing Glucose from Granular Starch Sustrates
Est. expiryNov 21, 2023(expired)· nominal 20-yr term from priority
Inventors:Toby M. BaldwinBenjamin S. BowerGopal K. ChotaniNigel Dunn-ColemanOreste J. Lantero, Jr.Suzanne E. LantzMichael J. PepsinJayarama K. ShettyBruce A. Strohm
C12N 9/2428C12N 1/14C12P 19/14C12P 19/20C12P 19/04C12P 19/24Y02P20/52C12N 9/242C12P 19/02C12N 9/2408C08B 30/00Y02E50/10
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Claims
Abstract
The present invention relates to filamentous fungal host cells and particularly Trichoderma host cells useful for the production of heterologous granular starch hydrolyzing enzymes having glucoamylase activity (GSHE). Further the invention relates to a method for producing a glucose syrup comprising contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a GSHE at a temperature equal to or below the gelatinization temperature of the granular starch to obtain a composition of a glucose syrup.
Claims
exact text as granted — not AI-modified1 . A fermentation medium comprising a granular starch hydrolyzing enzyme having glucoamylase activity (GSHE) produced from a culture of Trichoderma reesei , wherein the Trichoderma reesei comprises a heterologous polynucleotide encoding a GSHE having at least 90% amino acid sequence identity with SEQ ID NO: 3 or with SEQ ID NO: 6.
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