US2013136742A1PendingUtilityA1

Peptide having cell membrane penetrating activity

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Assignee: LEE KYUNGLIMPriority: Feb 20, 2006Filed: Feb 1, 2013Published: May 30, 2013
Est. expiryFeb 20, 2026(expired)· nominal 20-yr term from priority
Inventors:Kyunglim LeeYoungjoo KwonMiyoung KimMoonhee Kim
C07K 14/46C07K 7/06C07K 16/18A61K 47/64C07K 14/435A61K 48/0008A61K 47/65C07K 14/47C07K 7/08A61P 43/00C07K 7/00
51
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Claims

Abstract

Provided are transmembrane complexes that contain a protein transduction domain (PTD) from the N-terminus of IgE-dependent histamine-releasing factor (HRF) and a target substance that is to be delivered into a cell. Also provided are nucleic acid molecules encoding the transmembrane complex, and methods of delivering the target substance into a cell interior by contacting the transmembrane complex with a cell. Also provided are transfection kits containing the PTD and the target substance.

Claims

exact text as granted — not AI-modified
What is claimed: 
     
         1 . An isolated peptide that consists of a sequence of amino acids selected from among SEQ ID NO: 2; SEQ ID NO: 3; SEQ ID NO:4, SEQ ID NO: 12; SEQ ID NO: 13; SEQ ID NO: 14; SEQ ID NO: 15; SEQ ID NO:16, SEQ ID NO: 22; SEQ ID NO: 26; SEQ ID NO: 27; SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46, SEQ ID NO: 47, SEQ ID NO: 48; SEQ ID NO: 49, SEQ ID NO: 50, SEQ ID NO: 51, SEQ ID NO: 52, SEQ ID NO: 53 and SEQ ID NO: 54, wherein the isolated peptide has cell membrane penetrating activity. 
     
     
         2 . The peptide of  claim 1 , wherein the sequence of amino acids is selected from among one of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15 and SEQ ID NO: 16. 
     
     
         3 . The peptide of  claim 1 , wherein the sequence of amino acids is selected from among one of SEQ ID NO: 22, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42,SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46, SEQ ID NO: 47,SEQ ID NO: 48, SEQ ID NO: 49, SEQ ID NO: 50, SEQ ID NO: 51, SEQ ID NO: 52, SEQ ID NO: 53 and SEQ ID NO: 54. 
     
     
         4 . A transmembrane complex, comprising the peptide of  claim 1  and a target substance. 
     
     
         5 . A transmembrane complex, consisting of the peptide of  claim 1  and a target substance. 
     
     
         6 . The transmembrane complex of  claim 4 , wherein the target substance is selected from among a nucleic acid, a drug, a chemical compound, a carbohydrate, a lipid, a glycolipid, an enzyme, a regulating factor, a growth factor and an antibody. 
     
     
         7 . The transmembrane complex of  claim 4  that is a fusion protein, comprising the peptide of  claim 1  linked to the target substance directly or via a linker. 
     
     
         8 . The transmembrane complex of  claim 4 , wherein the peptide of  claim 1  is linked to the target substance via a linker. 
     
     
         9 . A transfection kit, comprising: a first composition containing the peptide of  claim 1 . 
     
     
         10 . The transfection kit of  claim 9 , further comprising a target substance. 
     
     
         11 . The transfection kit of  claim 10 , further comprising a binding factor for linking the peptide to the target substance. 
     
     
         12 . A method for delivering a target substance into a cell interior, comprising:
 administering a transmembrane complex to a subject, wherein the complex comprises a peptide of  claim 1  and a target substance.   
     
     
         13 . The method of  claim 12 , wherein the target substance is selected from among a nucleic acid, a drug, a chemical compound, a carbohydrate, a lipid, a glycolipid, an enzyme, a regulating factor, a growth factor and an antibody. 
     
     
         14 . A nucleic acid molecule encoding a sequence of amino acids selected from among SEQ ID NO: 2; SEQ ID NO: 3; SEQ ID NO:4, SEQ ID NO: 12; SEQ ID NO: 13; SEQ ID NO: 14; SEQ ID NO: 15; SEQ ID NO:16, SEQ ID NO: 22; SEQ ID NO: 26; SEQ ID NO: 27; SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46, SEQ ID NO: 47, SEQ ID NO: 48; SEQ ID NO: 49, SEQ ID NO: 50, SEQ ID NO: 51, SEQ ID NO: 52, SEQ ID NO: 53 and SEQ ID NO: 54. 
     
     
         15 . The nucleic acid molecule of  claim 14 , wherein the nucleic acid molecule is one selected from among SEQ ID NO: 18 and 55-81. 
     
     
         16 . A nucleic acid molecule encoding a transmembrane complex, comprising a nucleic acid molecule of  claim 14 . 
     
     
         17 . The nucleic acid molecule of  claim 16 , further comprising a nucleic acid molecule encoding a target substance selected from an enzyme, a regulating factor, a growth factor, or an antibody. 
     
     
         18 . The nucleic acid molecule of  claim 17 , further comprising a nucleic acid molecule encoding a linker. 
     
     
         19 . A method of delivering a target substance into a cell interior, comprising
 (a) linking a protein transduction domain (PTD) to a target substance by physically/chemically covalent bond or non-covalent bond or by mediator in incorporated or fused form to form a transmembrane complex; and   (b) administering the transmembrane complex to a subject,   wherein the PTD comprises a sequence of amino acids selected from among SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO:4, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO:16, SEQ ID NO: 22, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46, SEQ ID NO: 47, SEQ ID NO: 48; SEQ ID NO: 49, SEQ ID NO: 50, SEQ ID NO: 51, SEQ ID NO: 52, SEQ ID NO: 53 and SEQ ID NO: 54.   
     
     
         20 . The method of  claim 19 , wherein the target substance is selected from among a nucleic acid, a drug, a chemical compound, a carbohydrate, a lipid, a glycolipid, an enzyme, a regulating factor, a growth factor and an antibody.

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