Plant Stem Cell Line Derived from Cambium of Herbaceous Plant with Storage Root and Method for Isolating the Same

Abstract

The present invention relates to a cell line derived from the cambium of an herbaceous plant having a storage root and a method for isolating the same. More specifically, relates to a cambium-derived homogeneous cell line having the ability to divide, which is obtained from the cambium-containing storage tissue of an herbaceous plant having a storage root without a separate dedifferentiation process, and to a method for isolating the same. The cell line derived from the cambium of an herbaceous plant having a storage root has an active division ability and is homogeneous. Also, it is stable during culture, because it has not undergone a dedifferentiation process. Thus, through the optimization of proliferation thereof, the cell line can be allowed to proliferate in a large amount within a short time.

1 - 11 . (canceled) 
     
     
         12 . A method for isolating a cell line derived from the cambium of an herbaceous plant having a storage root, the method comprising:
 (a) obtaining storage root tissue of an herbaceous plant having a storage root, wherein the tissue comprises cambium;   (b) treating the tissue such that differentiated, non-cambium cells lose the ability to divide;   (c) inducing cambium cell proliferation by culturing the obtained cambium-containing storage root tissue in an induction medium comprising a cambium-division hormone; and   (d) collecting the induced cambium-derived cell line.   
     
     
         13 . The method of  claim 12 , wherein the herbaceous plant is selected from the group consisting of ginseng,  Codonopsis lanceolata, Ostericum koreanum  KITAGAWA,  Platycodon grandiflorum, Pueraria thunbergiana, Aralia contonentalis  Kitagawa,  Ledebouriella seseloides, Angelica gigas  NAKAI, carrot, sweet potato, Maca, and cassava. 
     
     
         14 . A method for isolating a cell line derived from the cambium of a ginseng root, the method comprising:
 (a) obtaining root tissue of a ginseng plant, wherein the tissue comprises cambium;   (b) treating the tissue such that differentiated, non-cambium cells lose the ability to divide;   (c) inducing cambium cell proliferation by culturing the obtained cambium-containing root tissue in an induction medium comprising a cambium-division hormone; and   (d) collecting the induced cambium-derived cell line.   
     
     
         15 . The method of  claim 14 , wherein the ginseng plant is selected from the group consisting of cultivated ginseng and true wild ginseng. 
     
     
         16 . The method of  claim 12 , wherein the cambium-division hormone is auxin. 
     
     
         17 . The method of  claim 12 , wherein the cambium-division hormone is indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), or a combination thereof. 
     
     
         18 . The method of  claim 12 , wherein said induction medium comprises IAA or IBA at a concentration of about 0.1 to about 5 mg/l. 
     
     
         19 . The method of  claim 12 , wherein the treatment of (b) comprises subjecting the tissue to an agent which produces osmotic stress. 
     
     
         20 . The method of  claim 12 , further comprising transferring the cambium-derived cell line to a maintenance medium which supports continued proliferation of said cambium-derived cell line. 
     
     
         21 . The method of  claim 20 , wherein said maintenance medium comprises auxin. 
     
     
         22 . The method of  claim 21 , wherein said maintenance medium comprises one or more of 2,4-dichlorophenoxyacetic acid (2,4-D), picloram, or IBA. 
     
     
         23 . The method of  claim 22 , wherein said maintenance medium comprises 2,4-D, picloram or IBA at a concentration of about 1 to about 5 mg/L. 
     
     
         24 . The method of  claim 14 , further comprising preserving said cambium-derived cell line by freezing, wherein said cambium-derived cell line can be thawed and recultured.