Reagent splitting/dispensing method based on reagent dispensing nozzle and reagent splitting/dispensing mechanism

Abstract

A reagent splitting/dispensing method based on a reagent dispensing nozzle controls a split or dispensation amount of reagent, includes a waiting process of disposing a first air layer between an interface of the operation fluid and a nozzle tip end in the reagent dispensing nozzle; a first moving process of moving the reagent dispensing nozzle to a position above the reagent to be split; a second moving process of depositing the nozzle tip end in the reagent; an air layer adjusting process of increasing the occupation amount of the operation fluid in the reagent dispensing nozzle and decreasing the occupation amount of the first air layer, between the first moving process and the second moving process; and a reagent splitting process of decreasing the occupation amount of the operation fluid in the reagent dispensing nozzle and filling the reagent into the reagent dispensing nozzle from the nozzle tip end.

What is claimed is: 
     
         1 . A reagent splitting/dispensing method based on a reagent dispensing nozzle that controls a split amount or a dispensation amount of a reagent by an operation fluid disposed in the reagent dispensing nozzle, the reagent splitting/dispensing method comprising:
 a waiting process of disposing a first air layer between an interface of the operation fluid and a nozzle tip end in the reagent dispensing nozzle;   a first moving process of moving the reagent dispensing nozzle to a position above the reagent to be split;   a second moving process of depositing the nozzle tip end in the reagent;   an air layer adjusting process of increasing the occupation amount of the operation fluid in the reagent dispensing nozzle and decreasing the occupation amount of the first air layer, between the first moving process and the second moving process;   a reagent splitting process of decreasing the occupation amount of the operation fluid in the reagent dispensing nozzle and filling the reagent into the reagent dispensing nozzle from the nozzle tip end.   
     
     
         2 . The reagent splitting/dispensing mechanism comprising:
 a triaxial actuator in which a movement axis in a horizontal direction is set to an X axis and a Y axis and a movement axis in a vertical direction is set to a Z axis;   a reagent dispensing nozzle which is moved by the triaxial actuator;   a pump unit which is connected to the reagent dispensing nozzle and controls an operation fluid disposed in the reagent dispensing nozzle; and   a control unit which outputs a first air layer arrangement signal to decrease the occupation amount of the operation fluid in the nozzle and dispose a first air layer between an interface of the operation fluid and a nozzle tip end in the reagent dispensing nozzle to the pump unit, outputs a first movement signal to move the reagent dispensing nozzle to a position above the reagent to be split and a second movement signal to deposit the nozzle tip end in the reagent to the triaxial actuator after the first air layer is disposed in the reagent dispensing nozzle, outputs an air layer adjustment signal to increase the occupation amount of the operation fluid in the reagent dispensing nozzle to the pump unit, between the output of the first movement signal and the output of the second movement signal, outputs a reagent split signal to decrease the occupation amount of the operation fluid in the nozzle and fill the reagent into the reagent dispensing nozzle from the nozzle tip end to the pump unit after the nozzle tip end is deposited in the reagent.