Chimeric gene with several herbicide tolerance genes, plant cell and plant resistant to several herbicides

Abstract

Methods for producing plants with multiple herbicide resistance are provided, in which the plants contain at least two basic genes, the first basic gene comprising a coding sequence encoding a mutated HPPD, and the second basic gene encoding an enzyme conferring on plants tolerance to a herbicide. Methods for herbicidal treatment of plants are also provided.

1 . A process for producing plants with multiple herbicide tolerance by transgenesis of the plants, comprising
 inserting into several cells respectively one of at least two basic genes, the basic genes each containing regulatory elements necessary for its transcription in plants and a coding sequence encoding an enzyme conferring on plants tolerance to a herbicide, wherein said at least two basic genes comprise first and second basic genes, said first basic gene comprising a coding sequence encoding a mutated HPPD and said second basic gene comprising a second coding sequence encoding an enzyme conferring on plants tolerance to a herbicide;   regenerating plants from said cells; and   crossing said plants in order to obtain plants with multiple tolerance to field application rates of said herbicides.   
     
     
         2 . A process for the herbicidal treatment of plants comprising applying at least two herbicides at field application rates to a plant comprising at least two basic genes comprising first and second basic genes, said first basic gene comprising regulatory elements necessary for its transcription in plants and a coding sequence encoding a mutated HPPD, and said second basic gene comprising regulatory elements necessary for its transcription in plants and a second coding sequence encoding an enzyme conferring on plants tolerance to a herbicide. 
     
     
         3 . The process according to  claim 2 , characterized in that three herbicides are applied. 
     
     
         4 . The process according to  claim 2 , characterized in that one of the herbicides is an HPPD inhibitor. 
     
     
         5 . The process according to  claim 2 , characterized in that both herbicides are applied simultaneously. 
     
     
         6 . The process according to  claim 5 , characterized in that both herbicides are applied in the form of a single composition ready to use. 
     
     
         7 . The process according to  claim 5 , characterized in that both herbicides are applied in the form of a freshly prepared mixture. 
     
     
         8 . The process according to  claim 2 , characterized in that both herbicides are applied in succession. 
     
     
         9 . The process according to  claim 4 , characterized in that the herbicide inhibiting HPPD is isoxaflutole. 
     
     
         10 . The process according to  claim 4 , characterized in that the herbicide inhibiting HPPD is sulcotrione. 
     
     
         11 . The process according to  claim 2 , characterized in that the herbicide belongs to the dihydroohydroxybenzonitrile family. 
     
     
         12 . The process according to  claim 11 , characterized in that the herbicide is bromoxynil. 
     
     
         13 . The process according to  claim 2 , characterized in that the herbicide inhibiting EPSPS is glyphosate. 
     
     
         14 . The process according to  claim 11 , characterized in that the herbicide is ioxynil. 
     
     
         15 . The process according to  claim 1 , wherein the second coding sequence encodes tolerance to glyphosate. 
     
     
         16 . The process according to  claim 1 , wherein the second coding sequence encodes an EPSPS conferring tolerance to glyphosate. 
     
     
         17 . The process according to  claim 1 , wherein the second coding sequence encodes glyphosate oxidoreductase. 
     
     
         18 . The process according to  claim 1 , wherein the second coding sequence encodes tolerance to phosphinothricin. 
     
     
         19 . The process according to  claim 1  wherein said second coding sequence encodes tolerance to a herbicide from the dihalohydroxybenzonitrile family. 
     
     
         20 . The process according to  claim 19  wherein said herbicide is bromoxynil. 
     
     
         21 . The process according to  claim 19 , wherein said herbicide is ioxynil. 
     
     
         22 . A process for producing transgenic plants with multiple herbicide tolerance comprising
 crossing a transgenic plant comprising at least one basic gene comprising a coding sequence encoding a mutated HPPD with another transgenic plant comprising at least a second basic gene comprising a second coding sequence encoding an enzyme conferring on plants tolerance to a herbicide, in order to obtain plants with multiple tolerance to field application rates of these herbicides.