US2013157900A1PendingUtilityA1

Structure-activity relationships

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Assignee: CODEXIS INCPriority: Feb 12, 2007Filed: Feb 11, 2013Published: Jun 20, 2013
Est. expiryFeb 12, 2027(~0.6 yrs left)· nominal 20-yr term from priority
Inventors:Emily MundorffSimon Christopher DavisGjalt W. HuismanAnke KrebberJohn H. Grate
G16B 20/50G16B 20/30C40B 30/04G01N 33/6803G16B 20/00C12N 15/1093C12N 9/00G01N 33/6845C40B 10/00C12Q 1/25C40B 40/10C40B 40/02C12Q 1/26C12Q 1/00
72
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Claims

Abstract

The present disclosure relates to compositions and methods for screening a plurality of polypeptide variants.

Claims

exact text as granted — not AI-modified
We claim: 
     
         1 . A kit for identifying at least one polypeptide having a desired activity, comprising:
 (i) at least one plurality of addressable polypeptides, wherein each member of each plurality reacts with at least one ligand to produce a detectable signal, and wherein at least two members of each plurality are related to a parent polypeptide and react with different ligands; and   (ii) instructions for use.   
     
     
         2 . The kit of  claim 1 , wherein said at least one ligand is placed on a surface. 
     
     
         3 . The kit of  claim 2 , wherein said surface is selected from plastic, glass, silica, metal, microparticles, microchips, and beads. 
     
     
         4 . The kit of  claim 3 , wherein said at least one ligand is immobilized on said surface. 
     
     
         5 . The kit of  claim 3 , wherein said plastic comprises the wells of a microtiter plate. 
     
     
         6 . The kit of  claim 5 , wherein the wells of said microtiter plate contain at least one ligand. 
     
     
         7 . The kit of  claim 6 , wherein the wells of said microtiter plate contain different ligands. 
     
     
         8 . The kit of  claim 7 , wherein said at least one ligand is immobilized in at least one well of said microtiter plate. 
     
     
         9 . The kit of  claim 6 , wherein said at least one ligand is in solution in said wells of said microtiter plate. 
     
     
         10 . The kit of  claim 1 , wherein said at plurality of addressable polypeptides is placed on a surface. 
     
     
         11 . The kit of  claim 10 , wherein said surface is selected from plastic, glass, silica, metal, microparticles, microchips, and beads. 
     
     
         12 . The kit of  claim 10 , wherein at least one member of said plurality of addressable polypeptides is immobilized on said surface. 
     
     
         13 . The kit of  claim 11 , wherein said plastic comprises the wells of a microtiter plate. 
     
     
         14 . The kit of  claim 13 , wherein the wells of said microtiter plate contain at least one member of said plurality of addressable polypeptides. 
     
     
         15 . The kit of  claim 6 , wherein the wells of said microtiter plate contain different members of said plurality of addressable polypeptides. 
     
     
         16 . The kit of  claim 15 , wherein at least one member of said plurality of addressable polypeptides is immobilized in at least one well of said microtiter plate. 
     
     
         17 . The kit of  claim 14 , wherein said at least one member of said plurality of addressable polypeptide is in solution in said wells of said microtiter plate. 
     
     
         18 . The kit of  claim 1 , wherein said at least two members of each plurality of addressable polypeptides are related to a parent polypeptide and react with differing levels of activity upon said at least one ligand. 
     
     
         19 . The kit of  claim 1 , further comprising at least one means of generating a signal. 
     
     
         20 . The kit of  claim 19 , further comprising at least one means of detecting said signal.

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