Specific binding agents of human angiopoietin-2

Abstract

Disclosed are peptides that bind to Ang-2. Also disclosed are peptibodies comprising the peptides, methods of making such peptides and peptibodies, and methods of treatment using such peptides and peptibodies.

1 .- 117 . (canceled) 
     
     
         118 . A polypeptide capable of binding Ang-2, wherein the polypeptide comprises an amino acid sequence 
       
         
           
                 
                 
                 
               
                     
                   QKYQPLDELDKTLYDQFMLQQG 
                   [SEQ ID NO: 131] 
                 
             
                
               
            
           
         
         wherein up to two amino acids therein are substituted by an unconventional amino acid. 
       
     
     
         119 . A polypeptide according to  claim 118 , wherein two amino acids in said sequence are substituted by an unconventional amino acid. 
     
     
         120 . A polypeptide according to  claim 119 , wherein both of the unconventional amino acids are ε-acetyl lysine. 
     
     
         121 . A polypeptide according to  claim 118 , further comprising an Fc domain. 
     
     
         122 . A polypeptide capable of binding Ang-2, wherein the polypeptide comprises an amino acid sequence 
       
         
           
                 
                 
                 
               
                     
                   QKYQPLDELDKTLYDQFMLQQG 
                   [SEQ ID NO: 131] 
                 
             
                
               
            
           
         
         wherein up to two amino acids therein are substituted by a derivative amino acid. 
       
     
     
         123 . A polypeptide according to  claim 122 , wherein up to one of the amino acids is substituted by a derivative amino acid and up to one is substituted by an unconventional amino acid. 
     
     
         124 . A polypeptide according to  claim 122 , comprising one or two derivative amino acids, wherein one or both derivative amino acids have a modified side chain. 
     
     
         125 . A polypeptide according to  claim 123 , wherein the derivative amino acid has a modified side chain. 
     
     
         126 . A polypeptide according to  claim 125 , wherein the unconventional amino acid is ε-acetyl lysine. 
     
     
         127 . A polypeptide according to  claim 122 , further comprising an Fc domain. 
     
     
         128 . A specific binding agent, comprising a peptide of SEQ ID NO: 131 with up to 2 amino acid substitutions, wherein each of said amino acid substitutions is an unconventional amino acid substitution, wherein each unconventional amino acid substitution is an ε-acetyl lysine amino acid, and wherein said peptide comprising said substitutions specifically binds to Ang2. 
     
     
         129 . A polypeptide according to  claim 128 , further comprising an Fc domain. 
     
     
         130 . A specific binding agent, comprising a peptide of SEQ ID NO: 131 wherein exactly one amino acid of said peptide of SEQ ID NO: 131 is a derivative amino acid, wherein said derivative peptide further comprises exactly one unconventional amino acid substitution, and wherein said peptide comprising said substitution specifically binds to Ang2. 
     
     
         131 . The specific binding agent of  claim 130 , wherein said derivative amino acid comprises a modified side chain. 
     
     
         132 . A polypeptide according to  claim 130 , further comprising an Fc domain. 
     
     
         133 . The specific binding agent of  claim 130 , wherein said unconventional amino acid substitution is an ε-acetyl lysine amino acid. 
     
     
         134 . The specific binding agent of  claim 133 , wherein said derivative amino acid comprises a modified side chain.