Method of inhibiting angiogenesis or invasion or formation of metastases

Abstract

A method of treating a mammal with a tumor by decreasing intratumoral vessels associated with tumor or inhibiting formation of new intratumoral vessels includes injecting tumor with therapeutically effective amount of expression plasmid including polynucleotide coding for therapeutic peptide consisting of SEQ ID NO: 2 absent any operably linked coding sequence, wherein polynucleotide sequence is operably linked to promoter or expression control sequence, followed by application of electric pulses to site of injection in tumor or injecting muscle of mammal with therapeutically effective amount of expression plasmid including polynucleotide coding for therapeutic peptide consisting of SEQ ID NO: 2 absent any operably linked coding sequence, wherein polynucleotide sequence is operably linked to promoter or expression control sequence, followed by applying electric pulses to site of injection in muscle of mammal, whereby expression of SEQ ID NO: 2 decreases intratumoral vessels associated with tumor, or formation of new intratumoral vessels therein.

1 . A method of treating a mammal with a tumor by decreasing intratumoral vessels associated with the tumor or inhibiting formation of new intratumoral vessels comprising:
 a) injecting the tumor with a therapeutically effective amount of an expression plasmid comprising a polynucleotide coding for a therapeutic peptide consisting of SEQ ID NO: 2 absent any operably linked to a promoter or an expression control sequence, followed by application of electric pulses to a site of the injection in the tumor; or   b) injecting a muscle of the mammal with a therapeutically effective amount of an expression plasmid comprising a polynucleotide coding for a therapeutic peptide consisting of SEQ ID NO: 2 absent any operably linked coding sequence, wherein the polynucleotide sequence is operably linked to a promoter or an expression control sequence, followed by applying electric pulses to a site of the injection in the muscle of the mammal,   whereby expression of SEQ ID NO: 2 decreases intratumoral vessels associated with the tumor, or formation of new intratumoral vessels therein.   
     
     
         2 . The method according to  claim 1 , wherein said polynucleotide sequence consists of SEQ ID NO: 1. 
     
     
         3 . The method according to  claim 1 , wherein said expression plasmid coding for the therapeutic peptide consisting of SEQ ID NO: 2 is administered by injection in said muscle of the mammal with a tumor followed by application of electric pulses to the site of injection in said muscle of the mammal with a tumor. 
     
     
         4 . A method of treating a mammal with metastases by decreasing intratumoral vessels associated with the metastases or inhibiting formation of new intratumoral vessels comprising:
 a) injecting a muscle of the mammal with a therapeutically effective amount of an expression plasmid comprising a polynucleotide coding for a therapeutic peptide consisting of SEQ ID NO: 2 absent any operably linked coding sequence, wherein the polynucleotide sequence is operably linked to a promoter or an expression control sequence;   b) followed by applying electric pulses to a site of the injection in the muscle,   whereby expression of SEQ ID NO: 2 decreases intratumoral vessels associated with the metastases or formation of new intratumoral vessels.   
     
     
         5 . The method according to  claim 4 , wherein said polynucleotide sequence consists of SEQ ID NO: 1. 
     
     
         6 . The method according to  claim 4 , wherein said expression plasmid coding for the therapeutic peptide consisting of SEQ ID NO: 2 is administered by injection in said muscle of the mammal with metastases followed by application of electric pulses to the site of injection in said muscle of the mammal with metastases.