Carrier polymer particle, process for producing the same, magnetic particle for specific trapping, and process for producing the same

Abstract

Carrier polymer particles comprising organic polymer particles having a particle diameter of 0.1 to 20 micrometers and a saccharide with which the surface of the organic polymer particles is covered, the organic polymer particles and the saccharide being chemically bonded.

1 . A process for producing a magnetic particle composition comprising:
 chemically bonding at least one magnetic particle having a diameter of 0.1 to 20 micrometers to a saccharide and   chemically bonding a probe for specifically trapping a target material to the saccharide, wherein the probe is bonded to the saccharide of the magnetic particle after treatment of the magnetic particle with a basic solution.   
     
     
         2 . The process according to  claim 1 ,
 wherein when chemically bonding the magnetic particles and the saccharide, the at least one magnetic particle has a first functional group and the saccharide has a second functional group, and the at least one magnetic particle and the saccharide are chemically bonded by reacting the first functional group and the second functional group.   
     
     
         3 . The process according to  claim 2 , wherein the first functional group is at least one functional group selected from the group consisting of a carboxyl group, an epoxy group, an amino group, and a tosyl group. 
     
     
         4 . The process according to  claim 1 , wherein the saccharide is a polysaccharide. 
     
     
         5 . The process according to  claim 1 , wherein the saccharide is carboxymethylated. 
     
     
         6 . The process according to  claim 1 , wherein the magnetic particle and the saccharide are chemically bonded by a bonding group which comprises at least one selected from the group consisting of an amide bond and an ester bond. 
     
     
         7 . The process according to  claim 1 , wherein the at least one magnetic particle is obtained by a process comprising polymerizing a polymer layer on a magnetic material layer comprising a mother particle, the mother particle comprising at least one nuclear particle and the magnetic material layer formed on the surface of the nuclear particle, and wherein
 the magnetic material layer comprises at least one of Fe 2 O 3  and Fe 3 O 4 .   
     
     
         8 . The process according to  claim 1 , wherein the probe is at least one probe selected from the group consisting of a protein, a peptide, a nucleic acid, a glycoside compound, and a synthetic chemical material. 
     
     
         9 . The process according to  claim 1 , wherein the saccharide and the probe are bonded by a chemical bond selected from the group consisting of an —O— bond, an —S— bond, an —SO— bond, an —SO 2 — bond, a —CO— bond, a —CO 2 — bond, an —NR— bond (wherein R is an alkyl group or H), an —N + R 2 R 3 — bond (wherein R 2  and R 3  are individually an alkyl group or H), an —NHCO— bond, and a —PO 2 — bond. 
     
     
         10 . The process according to  claim 1 , wherein the saccharide and the probe are bonded by a process comprising chemically reacting a functional group in the saccharide with a functional group in the probe. 
     
     
         11 . The process according to  claim 1 , wherein the functional group in the saccharide and the functional group in the probe are at least one selected from the group consisting of a hydroxyl group, an acyl group, a mercapto group, an amino group, an amino acyl group, a carbonyl group, a formyl group, a carboxyl group, an amide group, a sulfonic group, a phosphate group, an epoxy group, a tosyl group, an azido group, a vinyl group, and an allyl group. 
     
     
         12 . The process according to  claim 1 , wherein the target material is a biological material. 
     
     
         13 . The process according to  claim 1 , wherein the biological material is at least one selected from the group consisting of a protein, a peptide, a nucleic acid, a carbohydrate, a lipid, or another cellular substance. 
     
     
         14 . The process according to  claim 1 , wherein the other cellular substance is a blood-originating substance, a floating cell, a platelet, a erythrocyte, a leukocytes, or mixtures thereof. 
     
     
         15 . The process according to  claim 1 , wherein said particle is dispersed in a dispersion medium. 
     
     
         16 . A process of specific trapping comprising trapping a target material in the presence of the magnetic particle formed by the process of  claim 1 .