Novel glycosyl hydrolase enzymes and uses thereof

Abstract

The present disclosure is generally directed to glycosyl hydrolase enzymes, compositions comprising such enzymes, and methods of using the enzymes and compositions, for example for the saccharification of cellulosic and hemicellulosic materials into sugars.

1 . A non-naturally occurring composition comprising:
 a. a first polypeptide or a variant thereof having β-xylosidase activity, L-α-arabinofuranosidase activity, or both β-xylosidase activity and L-α-arabinofuranosidase activity, selected from a Fv43D, Fv3A, Pf43A, Fv43E, Fv39A, Fv43A, Fv43B, Pa51A, Fo43A, Gz43A,  Trichoderma reesei  Bxl1, Fv51A, Af43A, or Pf51A polypeptide; and   b. a second polypeptide or a variant thereof having xylanase activity, selected from a  Trichoderma  xylanase or an  Aspergillus  xylanase;   wherein the composition is capable of converting 60% or more of the hemicellulose xylan from a biomass into xylose.   
     
     
         2 - 4 . (canceled) 
     
     
         5 . The composition of  claim 1 , wherein the first polypeptide is selected from a Fv43D, Fv3A, Pf43A, Fv43E, Fv39A, Fo43A, Gz43A, or  T. reesei  Bxl1, or Fv43A, further comprising a third polypeptide or a variant thereof having L-α-arabinofuranosidase activity, selected from a Fv51A, Af43A, Fv43B, Pa51A or Pf51A polypeptide. 
     
     
         6 . (canceled) 
     
     
         7 . The composition of  claim 1 , further comprising a third polypeptide having β-xylosidase activity, wherein the first polypeptide is an Fv3A polypeptide or an Fv43A polypeptide, and the third polypeptide is an Fv43D, Pa51A, Gz43A,  Trichoderma reesei  Bxl1, Pf43A, Fv43E, Fv39A, Fo43A, or Fv43B polypeptide. 
     
     
         8 . (canceled) 
     
     
         9 . The composition of  claim 1 , wherein, if present:
 (a) the Fv43D polypeptide has at least 90% sequence identity to SEQ ID NO:28, or to residues (i) 20-341, (ii) 21-350, (iii) 107-341, or (iv) 107-350 of SEQ ID NO:28;   (b) the Fv3A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:2, or to residues (i) 24-766, (ii) 73-321, (iii) 73-394, (iv) 395-622, (v) 24-622, or (vi) 73-622 of SEQ ID NO:2;   (c) the Pf43A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:4, or to residues (i) 21-445, (ii) 21-301, (iii) 21-323, (iv) 21-444, (v) 302-444; (vi) 302-445, (vii) 324-444, or (viii) 324-445 of SEQ ID NO:4;   (d) the Fv43E polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:6, or to residues (i) 19-530, (ii) 29-530, (iii) 19-300, or (iv) 29-300 of SEQ ID NO:6:   (e) the Fv39A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:8, or to residues (i) 20-439, (ii) 20-291, (iii) 145-291, or 145-439 of SEQ ID NO:8:   (f) the Fv43A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:10, or to residues (i) 23-449, (ii) 23-302, (iii) 23-320, (iv) 23-448, (v) 303-448, or (vi) 321-449 of SEQ ID NO:10;   (g) the Fv43B polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:12, or to residues (i) 17-574, (ii) 27-574, (iii) 17-303, or (iv) 27-303 of SEQ ID NO:12;   (h) the Pa51A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:14, or to residues (i) 21-676, (ii) 21-652, (iii) 469-652, or (iv) 469-676 of SEQ ID NO:14;   (i) the Fo43A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:18, or to residues (i) 21-341, (ii) 107-341, (iii) 21-348, or (iv) 107-348 of SEQ ID NO:18;   (j) the Gz43A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:16, or to residues (i) 19-340, (ii) 53-340, (iii) 19-383, or (iv) 53-383 of SEQ ID NO:16:   (k) the  Trichoderma reesei  Bxl1 polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:44;   (l) the Fv51A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:32, or to residues (i) 21-660, (ii) 21-645, (iii) 450-645, or (iv) 450-660 of SEQ ID NO:32;   (m) the Af43A polypeptide has at least 90% sequence identity to SEQ ID NO:20, or to residues (i) 15-558, or (ii) 15-295 of SEQ ID NO:20; or   (n) the Pf51A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:22, or to residues (i) 21-632, (ii) 461-632, (iii) 21-642, or (iv) 461-642 of SEQ ID NO:22.   
     
     
         10 - 22 . (canceled) 
     
     
         23 . The composition of  claim 1 , wherein, if present:
 (a) the Fv43D polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:27, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:27, or to a fragment thereof;   (b) the Fv3A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:1, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:1, or to a fragment thereof;   (c) the Pf43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:3, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:3, or to a fragment thereof;   (d) the Fv43E polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:5, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:5, or to a fragment thereof;   (e) the Fv39A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:7, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:7, or to a fragment thereof;   (f) the Fv43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:9, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:9, or to a fragment thereof;   (g) the Fv43B polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:11, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:11, or to a fragment thereof;   (h) the Pa51A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:13, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:13, or to a fragment thereof;   (i) the Fo43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:17, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:17, or to a fragment thereof;   (j) the Gz43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:15, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:15, or to a fragment thereof;   (k) the Fv51A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:31, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:31, or to a fragment thereof;   (l) the Af43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:19, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:19, or to a fragment thereof; or   (m) the Pf51A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:21, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:21, or to a fragment thereof.   
     
     
         24 - 36 . (canceled) 
     
     
         37 . The composition of  claim 1 , wherein the  Trichoderma  xylanase is a  Trichoderma reesei  Xyn3 polypeptide, having at least 90% sequence identity to SEQ ID NO:42, or to residues 17-347 of SEQ ID NO:42, or is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:41, or by a nucleic acid that is capable of hybridizing under high stringency conditions to SEQ ID NO:41, or to a fragment thereof; or a  Trichoderma reesei  Xyn2 polypeptide, having at least 90% sequence identity to SEQ ID NO:43, or to residues 33-222 of SEQ ID NO:43. 
     
     
         38 - 40 . (canceled) 
     
     
         41 . The composition of  claim 1 , wherein the  Aspergillus  xylanase is an AfuXyn2 polypeptide, having at least 90% sequence identity to SEQ ID NO:24, or to residues 19-228 of SEQ ID NO:24, or is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:23, or by a nucleic acid that is capable of hybridizing under high stringency to SEQ ID NO:23, or to a fragment thereof; or an AfuXyn5 polypeptide, having at least 90% sequence identity to SEQ ID NO:26, or to residues 20-313 of SEQ ID NO:26, or is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:25, or by a nucleic acid that is capable of hybridizing under stringent conditions to a complement of SEQ ID NO:25, or to a fragment thereof. 
     
     
         42 - 45 . (canceled) 
     
     
         46 . The composition of  claim 1 , further comprising one or more polypeptides having cellulase activity. 
     
     
         47 . The composition of  claim 6 , wherein the one or more polypeptides having cellulase activity are independently components of a whole cellulase. 
     
     
         48 . The composition of  claim 7 , wherein the whole cellulase is a β-glucosidase-enriched whole cellulase. 
     
     
         49 . The composition of  claim 48 , wherein the whole cellulase is a filamentous fungal whole cellulase. 
     
     
         50 . The composition of  claim 48 , wherein the filamentous fungal whole cellulase is a preparation from an  Acremonium, Aspergillus, Emericella, Fusarium, Humicola, Mucor, Myceliophthora, Neurospora, Scytalidium, Thielavia, Chrysosporium, Phanerochaete, Tolypocladium , or  Trichoderma  species. 
     
     
         51 . The composition of  claim 48 , wherein the whole cellulase is a  Chrysosporium lucknowense, Phanerochaete chrysosporium, Trichoderma reesei, Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma viride , or  Pencillium funiculosum  whole cellulase. 
     
     
         52 . The composition of  claim 46 , wherein the one or more polypeptides having cellulase activity are selected from a β-glucosidase, an endoglucanase, and a cellobiohydrolase. 
     
     
         53 . The composition of  claim 52 , wherein the β-glucosidase is a  Trichoderma reesei  Bgl1 polypeptide, having at least 90% sequence identity to SEQ ID NO:45, or to residues 32-744 of SEQ ID NO:45, which constitutes up to 50 wt. % of the total weight of cellulase enzymes in the composition. 
     
     
         54 - 55 . (canceled) 
     
     
         56 . The composition of  claim 53 , wherein the β-glucosidase polypeptide constitutes 2 wt. % to 50 wt. % of the total weight of proteins in the composition. 
     
     
         57 . The composition of  claim 47 , wherein the one or more polypeptides having cellulase activity constitute 30 wt. % to 80 wt. % of the total weight of proteins in the composition. 
     
     
         58 . The composition of  claim 57 , wherein the one or more polypeptides having cellulase activity together are capable of achieving at least 0.00005 fraction product per mg protein per gram of phosphoric acid swollen cellulose (PASC) as determined by a calcofluor assay. 
     
     
         59 . The composition of  claim 1 , further comprising one or more accessory proteins, selected from mannanases, galactanases, arabinases, ligninases, amyloases, glucuronidases, proteases, esterases, lipases, xyloglucanases, glycoside hydrolase Family 61 polypeptides, CIP1, CIP2, CIP1-like proteins, CIP2-like proteins, cellobiose dehydrogenases, manganese peroxidases, swollenin, and expansins. 
     
     
         60 - 61 . (canceled) 
     
     
         62 . The composition of  claim 59 , wherein the one or more accessory proteins constitute 1 wt. % to 50 wt. % of the total weight of proteins in the composition. 
     
     
         63 . (canceled) 
     
     
         64 . The composition of  claim 1 , capable of converting 70% or more of the hemicellulose xylan from a biomass into xylose. 
     
     
         65 . The composition of  claim 64 , capable of converting 80% or more of the hemicellulose xylan from a biomass into xylose. 
     
     
         66 . The composition of  claim 1 , wherein the biomass is corncob, corn stover, corn fiber, switchgrass, sorghum, paper, pulp, or sugarcane bagasse. 
     
     
         67 . The composition of  claim 1 , wherein the biomass is  Miscanthus.    
     
     
         68 . The composition of  claim 1 , further comprising a biomass. 
     
     
         69 . The composition of  claim 68 , wherein the combined weight of polypeptides having xylanase activity is 1 g to 40 g per 1 kg of hemicellulose in the biomass. 
     
     
         70 . The composition of  claim 69 , wherein the combined weight of polypeptides having xylanase activity is 0.5 g to 40 g per 1 kg of hemicellulose in the biomass. 
     
     
         71 . The composition of  claim 70 , wherein the combined weight of polypetpides having xylanase activity is 0.5 g to 20 g per 1 kg of hemicellulose in the biomass. 
     
     
         72 . The composition of  claim 71 , wherein the combined weight of polypeptides having xylanase activity is 2 g to 20 g per 1 kg of hemicellulose in the biomass. 
     
     
         73 . The composition of  claim 68 , wherein the combined weight of polypeptides having β-xylosidase activity is 1 g to 50 g per 1 kg of hemicellulose in the biomass. 
     
     
         74 . The composition of  claim 73 , wherein the combined weight of polypeptides having β-xylosidase activity is 0.5 g to 50 g per 1 kg of hemicellulose in the biomass. 
     
     
         75 . The composition of  claim 74 , wherein the combined weight of polypeptides having β-xylosidase activity is 0.5 g to 40 g per 1 kg of hemicellulose in the biomass. 
     
     
         76 . The composition of  claim 75 , wherein the combined weight of polypeptides having β-xylosidase activity is 2 g to 40 g per 1 kg of hemicellulose in the biomass. 
     
     
         77 . The composition of  claim 68 , wherein the combined weight of polypeptides having L-α-arabinofuranosidase activity, if present, is 0.5 g to 20 g per 1 kg of hemicellulose in the biomass. 
     
     
         78 . The composition of  claim 77 , wherein the combined weight of polypetpides having L-α-arabinofuranosidase activity, if present, is 0.2 g to 20 g per 1 kg of hemicellulose in the biomass. 
     
     
         79 . The composition of  claim 68  further comprising one or more polypeptides having cellulase activity, wherein the combined weight of polypeptides having cellulase activity, if present, is 1 g to 100 g per 1 kg of cellulose in the biomass. 
     
     
         80 . A fermentation broth comprising the composition of  claim 1 . 
     
     
         81 . The fermentation broth of  claim 80 , which is the fermentation broth of a filamentous fungus. 
     
     
         82 . The fermentation broth of  claim 81 , wherein the filamentous fungus is a  Trichoderma, Humicola, Fusarium, Aspergillus, Neurospora, Penicillium, Cephalosporium, Achlya, Podospora, Endothia, Mucor, Cochliobolus, Pyricularia, Phanerochaete , or  Chrysosporium.    
     
     
         83 . The fermentation broth of  claim 82 , wherein the filamentous fungus is a  Aspergillus awamori, Aspergillus fumigatus, Aspergillus foetidus, Aspergillus japonicus, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae, Chrysosporium lucknowense, Fusarium bactridioides, Fusarium cerealis, Fusarium crookwellense, Fusarium culmorum, Fusarium graminearum, Fusarium graminum, Fusarium heterosporum, Fusarium negundi, Fusarium oxysporum, Fusarium reticulatum, Fusarium roseum, Fusarium sambucinum, Fusarium sarcochroum, Fusarium sporotrichioides, Fusarium sulphureum, Fusarium torulosum, Fusarium trichothecioides, Fusarium venenatum, Bjerkandera adusta, Ceriporiopsis aneirina, Ceriporiopsis aneirina, Ceriporiopsis caregiea, Ceriporiopsis gilvescens, Ceriporiopsis pannocinta, Ceriporiopsis rivulosa, Ceriporiopsis subrufa, Ceriporiopsis subvermispora, Coprinus cinereas, Coriolus hirsutus, Humicola insolens, Humicola lanuginosa, Mucor miehei, Myceliophthora thermophila, Neurospora crassa, Neurospora intermedia, Penicillium purpurogenum, Penicillium canescens, Penicillium solitum, Penicillium funiculosum, Phanerochaete chrysosporium, Phlebia radiate, Pleurotus eryngii, Talaromyces flavus, Thielavia terrestris, Trametes villosa, Trametes versicolor, Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma reesei , or  Trichoderma viride.    
     
     
         84 - 86 . (canceled) 
     
     
         87 . The fermentation broth of  claim 80 , which is a cell-free fermentation broth. 
     
     
         88 . A method of converting biomass to sugars, comprising contacting the biomass with a non-naturally occurring composition comprising:
 (a) a first polypeptide or a variant thereof having β-xylosidase activity, L-α-arabinofuranosidase activity, or both β-xylosidase activity and L-α-arabinofuranosidase activity, selected from a Fv43D, Fv3A, Pf43A, Fv43E, Fv39A, Fv43A, Fv43B, Pa51A, Fo43A, Gz43A,  Trichoderma reesei  Bxl1, Fv51A, Af43A, or Pf51A polypeptide; and   (b) a second polypeptide or a variant thereof having xylanase activity, selected from a  Trichoderma  xylanase or an  Aspergillus  xylanase.   
     
     
         89 . A saccharification process comprising treating a material comprising hemicellulose with a non-naturally occurring composition or a fermentation broth comprising:
 (a) a first polypeptide or a variant thereof having β-xylosidase activity, L-α-arabinofuranosidase activity, or both β-xylosidase activity and L-α-arabinofuranosidase activity, selected from a Fv43D, Fv3A, Pf43A, Fv43E, Fv39A, Fv43A, Fv43B, Pa51A, Fo43A, Gz43A,  Trichoderma reesei  Bxl1, Fv51A, Af43A, or Pf51A polypeptide; and   (b) a second polypeptide or a variant thereof having xylanase activity, selected from a  Trichoderma  xylanase or an  Aspergillus  xylanase.   
     
     
         90 . The process of  claim 89 , wherein the material comprising hemicellulose is corncob, corn stover, corn fiber, switchgrass, sorghum, paper, pulp,  Miscanthus , or sugarcane bagasse. 
     
     
         91 . (canceled) 
     
     
         92 . The process of  claim 89 , which yields at least 60% xylose from hemicellulose xylan of the material comprising hemicellulose. 
     
     
         93 . The process of  claim 89 , further comprising recovering monosaccharides. 
     
     
         94 - 119 . (canceled) 
     
     
         120 . A host cell engineered to recombinantly express a polypeptide:
 (a) having β-xylosidase activity, which has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:28, or to the amino acid sequence corresponding to residues (i) 20-341, (ii) 21-350, (iii) 107-341, or (iv) 107-350 of SEQ ID NO:28;   (b) having β-xylosidase activity, which has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:4, or to the amino acid sequence corresponding to residues (i) 21-445, (ii) 21-301, (iii) 21-323, (iv) 21-444, (v) 302-444, (vi) 302-445, (vii) 324-444, or (viii) 324-445 of SEQ ID NO:4;   (c) having β-xylosidase activity, which has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:6, or to the amino acid sequence corresponding to residues (i) 19-530, (ii) 29-530, (iii) 19-300, or (iv) 29-300 of SEQ ID NO:6;   (d) having β-xylosidase activity, which has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:8, or to the amino acid sequence corresponding to residues (i) 20-439, (ii) 20-291, (iii) 145-291, or (iv) 145-439 of SEQ ID NO:8;   (e) having β-xylosidase activity, which has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:10, or to the amino acid sequence corresponding to residues (i) 23-449, (ii) 23-302, (iii) 23-320, (iv) 23-448, (v) 303-448, (vi) 303-449, (vii) 321-448, or (viii) 321-449 of SEQ ID NO:10;   (f) having β-xylosidase activity, which has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:2, or to the amino acid sequence corresponding to residues (i) 24-766, (ii) 73-321, (iii) 73-394, (iv) 395-622, (v) 24-622, or (iv) 73-622 of SEQ ID NO:2;   (g) having β-xylosidase activity, which has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:16, or to the amino acid sequence corresponding to residues (i) 19-340, (ii) 53-340, (iii) 19-383, or (iv) 53-383 of SEQ ID NO:16;   (h) having β-xylosidase activity, which has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:18, or to the amino acid sequence corresponding to residues (i) 21-341, (ii) 107-341, (iii) 21-348, or (iv) 107-348 of SEQ ID NO:18;   (i) having β-xylosidase and/or L-α-arabinofuranosidase activities, which has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:12, or to the amino acid sequence corresponding to residues (i) 17-574, (ii) 27-574, (iii) 17-303, or (iv) 27-303 of SEQ ID NO:12;   (j) having β-xylosidase and/or L-α-arabinofuranosidase activities, which has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:14, or to the amino acid sequence corresponding to residues (i) 21-676, (ii) 21-652, (iii) 469-652, or (iv) 469-676 of SEQ ID NO:14;   (k) having L-α-arabinofuranosidase activity, which has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:20, or to the amino acid sequence corresponding to residues (i) 15-558, or (ii) 15-295 of SEQ ID NO:20;   (l) having L-α-arabinofuranosidase activity, which has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:22, or to the amino acid sequence corresponding to residues (i) 21-632, (ii) 461-632, (iii) 21-642, or (iv) 461-642 of SEQ ID NO:22; or   (m) having L-α-arabinofuranosidase activity, which has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:32, or to the amino acid sequence corresponding to residues (i) 21-660, (ii) 21-645, (iii) 450-645, or (iv) 450-660 of SEQ ID NO:32.   
     
     
         121 . The host cell of  claim 120 , which is a cell of a filamentous fungus. 
     
     
         122 . The host cell of  claim 121 , which is a cell of a  Trichoderma, Humicola, Fusarium, Aspergillus, Neurospora, Penicillium, Cephalosporium, Achlya, Podospora, Endothia, Mucor, Cochliobolus, Pyricularia, Phanerochaete , or  Chrysosporium.    
     
     
         123 . The host cell of  claim 122 , which is a cell of a  Aspergillus awamori, Aspergillus fumigatus, Aspergillus foetidus, Aspergillus japonicus, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae, Chrysosporium lucknowense, Fusarium bactridioides, Fusarium cerealis, Fusarium crookwellense, Fusarium culmorum, Fusarium graminearum, Fusarium graminum, Fusarium heterosporum, Fusarium negundi, Fusarium oxysporum, Fusarium reticulatum, Fusarium roseum, Fusarium sambucinum, Fusarium sarcochroum, Fusarium sporotrichioides, Fusarium sulphureum, Fusarium torulosum, Fusarium trichothecioides, Fusarium venenatum, Bjerkandera adusta, Ceriporiopsis aneirina, Ceriporiopsis aneirina, Ceriporiopsis caregiea, Ceriporiopsis gilvescens, Ceriporiopsis pannocinta, Ceriporiopsis rivulosa, Ceriporiopsis subrufa, Ceriporiopsis subvermispora, Coprinus cinereus, Coriolus hirsutus, Humicola insolens, Humicola lanuginosa, Mucor miehei, Myceliophthora thermophila, Neurospora crassa, Neurospora intermedia, Penicillium purpurogenum, Penicillium canescens, Penicillium solitum, Penicillium funiculosum, Phanerochaete chrysosporium, Phlebia radiate, Pleurotus eryngii, Talaromyces flavus, Thielavia terrestris, Trametes villosa, Trametes versicolor, Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma reesei , or  Trichoderma viride.    
     
     
         124 - 125 . (canceled) 
     
     
         126 . The host cell of  claim 120 , which is a cell of a bacterium. 
     
     
         127 . The host cell of  claim 126 , wherein the bacterium is a  Streptomyces , a  Thermomonospora , a  Bacillus , or a  Cellulomonas.    
     
     
         128 . A method of producing the composition of  claim 1 . 
     
     
         129 . (canceled) 
     
     
         130 . The process of  claim 89 , wherein the material comprising hemicellulose further comprises cellulose. 
     
     
         131 . The process of  claim 89 , wherein the amount of polypeptides having xylanase activity is 1 g to 40 g per kg of hemicellulose in the material. 
     
     
         132 . The process of  claim 131 , wherein the amount of polypeptides having xylanase activity is 0.5 g to 40 g per kg of hemicellulose in the material. 
     
     
         134 . The process of  claim 89 , wherein the amount of polypeptides having β-xylosidase activity is 1 g to 50 g per kg of hemicellulose in the material. 
     
     
         135 . The process of  claim 134 , wherein the amount of polypeptide having β-xylosidase activity is 0.5 g to 50 g per kg of hemicellulose in the material. 
     
     
         136 . The process of  claim 89 , wherein the amount of polypeptides having L-α-arabinofuranosidase activity is 0.5 g to 20 g per kg of hemicellulose in the material. 
     
     
         137 . The process of  claim 136 , wherein the amount of polypeptides having L-α-arabinofuranosidase activity is 0.2 g to 20 g per kg of hemicellulose in the material. 
     
     
         138 . The process of  claim 130 , wherein the amount of polypeptides having cellulase activity is 1 g to 100 g per kg of cellulose in the material comprising hemicellulose. 
     
     
         139 . The process of  claim 130 , wherein the amount of polypeptides having β-glucosidase activity is 50% or less of the total weight of polypeptides having cellulase activity. 
     
     
         140 . The composition of  claim 5 , wherein, if present:
 (a) the Fv43D polypeptide has at least 90% sequence identity to SEQ ID NO:28, or to residues (i) 20-341, (ii) 21-350, (iii) 107-341, or (iv) 107-350 of SEQ ID NO:28;   (b) the Fv3A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:2, or to residues (i) 24-766, (ii) 73-321, (iii) 73-394, (iv) 395-622, (v) 24-622, or (vi) 73-622 of SEQ ID NO:2;   (c) the Pf43A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:4, or to residues (i) 21-445, (ii) 21-301, (iii) 21-323, (iv) 21-444, (v) 302-444; (vi) 302-445, (vii) 324-444, or (viii) 324-445 of SEQ ID NO:4;   (d) the Fv43E polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:6, or to residues (i) 19-530, (ii) 29-530, (iii) 19-300, or (iv) 29-300 of SEQ ID NO:6;   (e) the Fv39A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:8, or to residues (i) 20-439, (ii) 20-291, (iii) 145-291, or 145-439 of SEQ ID NO:8;   (f) the Fv43A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:10, or to residues (i) 23-449, (ii) 23-302, (iii) 23-320, (iv) 23-448, (v) 303-448, or (vi) 321-449 of SEQ ID NO:10;   (g) the Fv43B polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:12, or to residues (i) 17-574, (ii) 27-574, (iii) 17-303, or (iv) 27-303 of SEQ ID NO:12;   (h) the Pa51A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:14, or to residues (i) 21-676, (ii) 21-652, (iii) 469-652, or (iv) 469-676 of SEQ ID NO:14;   (i) the Fo43A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:18, or to residues (i) 21-341, (ii) 107-341, (iii) 21-348, or (iv) 107-348 of SEQ ID NO:18;   (j) the Gz43A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:16, or to residues (i) 19-340, (ii) 53-340, (iii) 19-383, or (iv) 53-383 of SEQ ID NO:16;   (k) the  Trichoderma reesei  Bxl1 polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:44;   (l) the Fv51A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:32, or to residues (i) 21-660, (ii) 21-645, (iii) 450-645, or (iv) 450-660 of SEQ ID NO:32;   (m) the Af43A polypeptide has at least 90% sequence identity to SEQ ID NO:20, or to residues (i) 15-558, or (ii) 15-295 of SEQ ID NO:20; or   (n) the Pf51A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:22, or to residues (i) 21-632, (ii) 461-632, (iii) 21-642, or (iv) 461-642 of SEQ ID NO:22.   
     
     
         141 . The composition of  claim 7 , wherein, if present:
 (a) the Fv43D polypeptide has at least 90% sequence identity to SEQ ID NO:28, or to residues (i) 20-341, (ii) 21-350, (iii) 107-341, or (iv) 107-350 of SEQ ID NO:28;   (b) the Fv3A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:2, or to residues (i) 24-766, (ii) 73-321, (iii) 73-394, (iv) 395-622, (v) 24-622, or (vi) 73-622 of SEQ ID NO:2;   (c) the Pf43A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:4, or to residues (i) 21-445, (ii) 21-301, (iii) 21-323, (iv) 21-444, (v) 302-444; (vi) 302-445, (vii) 324-444, or (viii) 324-445 of SEQ ID NO:4;   (d) the Fv43E polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:6, or to residues (i) 19-530, (ii) 29-530, (iii) 19-300, or (iv) 29-300 of SEQ ID NO:6;   (e) the Fv39A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:8, or to residues (i) 20-439, (ii) 20-291, (iii) 145-291, or 145-439 of SEQ ID NO:8;   (f) the Fv43A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:10, or to residues (i) 23-449, (ii) 23-302, (iii) 23-320, (iv) 23-448, (v) 303-448, or (vi) 321-449 of SEQ ID NO:10;   (g) the Fv43B polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:12, or to residues (i) 17-574, (ii) 27-574, (iii) 17-303, or (iv) 27-303 of SEQ ID NO:12;   (h) the Pa51A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:14, or to residues (i) 21-676, (ii) 21-652, (iii) 469-652, or (iv) 469-676 of SEQ ID NO:14;   (i) the Fo43A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:18, or to residues (i) 21-341, (ii) 107-341, (iii) 21-348, or (iv) 107-348 of SEQ ID NO:18;   (j) the Gz43A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:16, or to residues (i) 19-340, (ii) 53-340, (iii) 19-383, or (iv) 53-383 of SEQ ID NO:16;   (k) the  Trichoderma reesei  Bxl1 polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:44;   (l) the Fv51A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:32, or to residues (i) 21-660, (ii) 21-645, (iii) 450-645, or (iv) 450-660 of SEQ ID NO:32;   (m) the Af43A polypeptide has at least 90% sequence identity to SEQ ID NO:20, or to residues (i) 15-558, or (ii) 15-295 of SEQ ID NO:20; or   (n) the Pf51A polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:22, or to residues (i) 21-632, (ii) 461-632, (iii) 21-642, or (iv) 461-642 of SEQ ID NO:22.   
     
     
         142 . The composition of  claim 5 , wherein, if present:
 (a) the Fv43D polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:27, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:27, or to a fragment thereof;   (b) the Fv3A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:1, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:1, or to a fragment thereof;   (c) the Pf43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:3, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:3, or to a fragment thereof;   (d) the Fv43E polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:5, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:5, or to a fragment thereof;   (e) the Fv39A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:7, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:7, or to a fragment thereof;   (f) the Fv43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:9, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:9, or to a fragment thereof;   (g) the Fv43B polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:11, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:11, or to a fragment thereof;   (h) the Pa51A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:13, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:13, or to a fragment thereof;   (i) the Fo43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:17, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:17, or to a fragment thereof;   (j) the Gz43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:15, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:15, or to a fragment thereof;   (k) the Fv51A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:31, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:31, or to a fragment thereof;   (l) the Af43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:19, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:19, or to a fragment thereof; or   (m) the Pf51A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:21, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:21, or to a fragment thereof.   
     
     
         143 . The composition of  claim 7 , wherein, if present:
 (a) the Fv43D polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:27, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:27, or to a fragment thereof;   (b) the Fv3A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:1, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:1, or to a fragment thereof;   (c) the Pf43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:3, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:3, or to a fragment thereof;   (d) the Fv43E polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:5, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:5, or to a fragment thereof;   (e) the Fv39A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:7, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:7, or to a fragment thereof;   (f) the Fv43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:9, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:9, or to a fragment thereof;   (g) the Fv43B polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:11, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:11, or to a fragment thereof;   (h) the Pa51A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:13, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:13, or to a fragment thereof;   (i) the Fo43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:17, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:17, or to a fragment thereof;   (j) the Gz43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:15, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:15, or to a fragment thereof;   (k) the Fv51A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:31, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:31, or to a fragment thereof;   (l) the Af43A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:19, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:19, or to a fragment thereof; or   (m) the Pf51A polypeptide is encoded by a nucleic acid having at least 90% sequence identity to SEQ ID NO:21, or by a nucleic acid that is capable of hybridizing, under high stringency conditions, to SEQ ID NO:21, or to a fragment thereof.