US2013197196A1PendingUtilityA1
Protein production
Est. expiryMar 2, 2027(~0.6 yrs left)· nominal 20-yr term from priority
A61P 35/00A61P 43/00A61P 35/04C07K 16/00C12Q 1/68C12N 15/85C07K 14/4702G01N 33/68C07K 2319/036C12N 15/67C12N 15/63C12P 21/00C07K 14/47C07K 14/435C12N 15/11A61P 31/00
46
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Claims
Abstract
The invention concerns the field of protein production and cell culture technology. CERT is identified as a novel in vivo PKD substrate. Phosphorylation on serine 132 by PKD decreases the affinity of CERT towards its lipid target phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity, identifying PKD as a regulator of lipid homeostasis. The present invention shows that CERT in turn is critical for PKD activation and PKD dependent protein cargo transport to the plasma membrane. The interdependence of PKD and CERT is thus a key to the maintenance of Golgi membrane integrity and secretory transport.
Claims
exact text as granted — not AI-modified1 - 38 . (canceled)
39 - 67 . (canceled)
68 . A protein of interest or an antibody produced in a cell by a method comprising:
a. increasing the expression or activity of a protein having an amino acid sequence comprising a steroidogenic acute regulatory related lipid transfer (START) domain or a derivative or mutant thereof; and b. effecting the expression of said protein of interest.
69 - 75 . (canceled)Cited by (0)
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