Soybean event 3560.4.3.5 and compositions and methods for the identification and/or detection thereof

Abstract

Compositions and methods related to transgenic glyphosate/ALS inhibitor-tolerant soybean plants are provided. Specifically, soybean plants having a 3560.4.3.5 event which imparts tolerance to glyphosate and at least one ALS-inhibiting herbicide are provided. The soybean plant harboring the 3560.4.3.5 event at the recited chromosomal location comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO:10 and/or 11. The characterization of the genomic insertion site of the 3560.4.3.5 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean 3560.4.3.5 events are provided.

1 .- 36 . (canceled) 
     
     
         37 . An isolated polynucleotide comprising SEQ ID NO: 11. 
     
     
         38 . The isolated polynucleotide of  claim 37 , wherein said polynucleotide is selected from the group consisting of:
 (a) a nucleotide sequence set forth in SEQ ID NO: 13, 15, 28, or 42; and,   (b) a nucleotide sequence comprising a fragment of SEQ ID NO: 13, 15, 28, or 42.   
     
     
         39 . A kit for identifying event 3560.4.3.5 in a biological sample, said kit comprising a first and a second primer, wherein said first and said second primer amplify a polynucleotide comprising a 3560.4.3.5 specific region, wherein said 3560.4.3.5 specific region comprises SEQ ID NO: 11. 
     
     
         40 . The kit of  claim 39 , wherein said kit further comprises a polynucleotide for the detection of the 3560.4.3.5 specific region. 
     
     
         41 . The kit of  claim 39 , wherein said first primer comprises a first fragment of SEQ ID NO: 6 and the second primer comprises a second fragment of SEQ ID NO:6, wherein said first and said second primer flank said 3560.4.3.5 specific region and share sufficient sequence homology or complementarity to said polynucleotide to amplify said 3560.4.3.5 specific region. 
     
     
         42 . The kit of  claim 41 , wherein
 a) said first primer comprises a fragment of SEQ ID NO:3 and said second primer comprises a fragment of SEQ ID NO:5; or,   b) said first primer comprises a fragment of SEQ ID NO: 4 and said second primer comprises a fragment of SEQ ID NO:5.   
     
     
         43 . The kit of  claim 41 , wherein said first and said second primer comprises at least 8 consecutive polynucleotides of SEQ ID NO: 6. 
     
     
         44 . The kit of  claim 42 , wherein said first or said second primer comprises at least 8 consecutive polynucleotides of SEQ ID NO:3, 4, or 5. 
     
     
         45 . The kit of  claim 39 , wherein said first or said second primer comprise SEQ ID NO:7, 8, 9, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 37, 38, 39, 40, 44, 45, 46, 51, 52, 53, 54, 55. 
     
     
         46 . A DNA detection kit comprising at least one polynucleotide that can specifically detect a 3560.4.3.5 specific region comprising SEQ ID NO: 11, wherein said polynucleotide comprises at least one DNA molecule of a sufficient length of contiguous nucleotides identical or complementary to SEQ ID NO: 6. 
     
     
         47 . The DNA detection kit of  claim 46 , wherein said polynucleotide that can specifically detect a 3560.4.3.5 specific region comprising a polynucleotide having SEQ ID NO: 11. 
     
     
         48 . The DNA detection kit of  claim 46 , wherein said polynucleotide comprises a sequence which hybridizes under stringent conditions with sequences comprising the sequences of SEQ ID NO:5 and SEQ ID NO:3. 
     
     
         49 . A method for identifying event 3560.4.3.5 in a biological sample, comprising
 (a) contacting said sample with a first and a second primer; and,   (b) amplifying a polynucleotide comprising a 3560.4.3.5 specific region comprising SEQ ID NO: 11.   
     
     
         50 . The method of  claim 49 , further comprising detecting the 3560.4.3.5 specific region. 
     
     
         51 . The method of  claim 49 , wherein said first primer comprises a first fragment of SEQ ID NO: 6 and the second primer comprises a second fragment of SEQ ID NO:6, wherein said first and said second primer flank said 3560.4.3.5 specific region and share sufficient sequence homology or complementarity to said polynucleotide to amplify said 3560.4.3.5 specific region. 
     
     
         52 . The method of  claim 51 , wherein
 a) said first primer comprises a fragment of SEQ ID NO:3 and said second primer comprises a fragment of SEQ ID NO:5; or,   b) said first primer comprises a fragment of SEQ ID NO: 5 and said second primer comprises a fragment of SEQ ID NO:4.   
     
     
         53 . The method of  claim 51 , wherein said first and said second primer comprise at least 8 consecutive polynucleotides of SEQ ID NO: 6. 
     
     
         54 . The method of  claim 52 , wherein said first and said second primer comprise at least 8 consecutive polynucleotides of SEQ ID NO:5, 4 or 3. 
     
     
         55 . The method of claim  13 , wherein said first and said second primer comprise SEQ ID NO:7, 8, 9, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 37, 38, 39 40, 44, 45, 46, 51, 52, 53, 54, or 55. 
     
     
         56 . A pair of DNA molecules comprising a first DNA molecule and a second DNA molecule, wherein said first DNA molecule comprising a first fragment of SEQ ID NO: 6 and the second DNA molecule comprising a second fragment of SEQ ID NO:6, wherein said first and said second DNA molecule flank said 3560.4.3.5 specific region comprising SEQ ID NO: 11 and share sufficient sequence homology or complementarity to said polynucleotide to amplify said 3560.4.3.5 specific region. 
     
     
         57 . The DNA detection kit of  claim 46 , wherein said polynucleotide comprises at least 14 consecutive identical or complementary nucleotides to SEQ ID NO: 6.