Sugarcane-stalk-sugar-content-related marker and the use thereof

Abstract

According to the present invention, a sugarcane-stalk-sugar-content-related marker linked to a sugarcane quantitative trait is provided. Such marker is a sugarcane-stalk-sugar-content-related marker, which comprises a continuous nucleic acid region existing in a region sandwiched between the nucleotide sequence shown in SEQ ID NO: 1 and the nucleotide sequence shown in SEQ ID NO: 8 or a region sandwiched between the nucleotide sequence shown in SEQ ID NO: 9 and the nucleotide sequence shown in SEQ ID NO: 16.

1 . A sugarcane-stalk-sugar-content-related marker, which consists of a continuous nucleic acid region existing in a region sandwiched between the nucleotide sequence shown in SEQ ID NO: 1 and the nucleotide sequence shown in SEQ ID NO: 8 or a region sandwiched between the nucleotide sequence shown in SEQ ID NO: 9 and the nucleotide sequence shown in SEQ ID NO: 16 of a sugarcane chromosome. 
     
     
         2 . The sugarcane-stalk-sugar-content-related marker according to  claim 1 , wherein the continuous nucleic acid region comprises any nucleotide sequence selected from the group consisting of the nucleotide sequences shown in SEQ ID NOS: 1 to 16. 
     
     
         3 . The sugarcane-stalk-sugar-content-related marker according to  claim 1 , wherein the continuous nucleic acid region is located at a position in a region sandwiched between the nucleotidesequence shown in SEQ ID NO: 1 and the nucleotide sequence shown in SEQ ID NO: 2 or a region sandwiched between the nucleotide sequence shown in SEQ ID NO: 13 and the nucleotide sequence shown in SEQ ID NO: 14 of a sugarcane chromosome. 
     
     
         4 . A method for producing a sugarcane line having an increased stalk sugar content comprising: a step of extracting a chromosome of a progeny plant obtained from parent plants, at least one of which is sugarcane; and a step of determining the presence or absence of the sugarcane-stalk-sugar-content-related marker according to  claim 1  in the obtained sugarcane chromosome. 
     
     
         5 . The method for producing a sugarcane line according to  claim 4 , wherein a DNA chip provided with probes each corresponding to the sugarcane-stalk-sugar-content-related marker is used in the determination step. 
     
     
         6 . The method for producing a sugarcane line according to  claim 4 , wherein the progeny plant is in the form of seeds or a young seedling and the chromosome is extracted from the seeds or the young seedling.