Substrate manufacturing method for sensor applications using optical characteristics and the substrate therefrom

Abstract

Nanoparticles which are uniform with respect to the distance between particles, their interval and arrangement, which are capable of being fixed on the solid substrate having a large surface greater than about 10×10 cm 2 using the Langmuir-Blodgett method. The nanofilm thus produced can be used as the substrate of an analytical sensor, and when the analytical sensitivity property is measured using the substrate of the analytical sensor thus prepared, it is seen that that the sensitivity or the sensor can be improved.

What is claimed is: 
     
         1 . A method for manufacturing a substrate of an analytical sensor, said method comprising:
 (a) preparing a dispersed solution of nanoparticles by surface-modifying said nanoparticles with an organic functional group and dispersing such surface-modified nanoparticles in a volatile organic solvent   (b) preparing a single layer film of the nanoparticles surface-modified with said organic functional group using said dispersed solution of nanoparticles on the basis of the Langmuir-Blodgett method, and then transferring said single layer film of nanoparticles to the substrate; and   (c) coating the substrate to which said single layer film of nanoparticles is transferred, with a metal thin film by means of the vacuum vapor deposition.   
     
     
         2 . The method for manufacturing the substrate of the analytical sensor according to  claim 1 , further comprising removing nanoparticles after coating with the metal thin film by means of the vacuum vapor deposition. 
     
     
         3 . The method for manufacturing the substrate of the analytical sensor according to  claim 1 , wherein said nanoparticles are silica particles. 
     
     
         4 . The method for manufacturing the substrate of the analytical sensor according to  claim 1 , wherein said organic functional group is a thiol group. 
     
     
         5 . The method for manufacturing the substrate of the analytical sensor according to  claim 1 , wherein said organic solvent is chloroform. 
     
     
         6 . The method for manufacturing the substrate of the analytical sensor according to  claim 1 , wherein said metal thin film is a gold thin film. 
     
     
         7 . The method for manufacturing the substrate of the analytical sensor according to  claim 1 , wherein said vacuum vapor deposition is the electron beam evaporation. 
     
     
         8 . The method for manufacturing the substrate of the analytical sensor according to  claim 3 , wherein said silica particles in step (a) are prepared by self-assembly of tetraethylorthosilicate (TEOS) using ammonia water as a catalyst. 
     
     
         9 . The method for manufacturing the substrate of the analytical sensor according to  claim 1 , wherein step (a) comprises:
 (a-1) preparing silica particles by self-assembly of an organic molecule comprising silicon using ammonia water as a catalyst;   (a-2) centrifuging silica particles as prepared in step (a-1) by means of a centrifuge immersing the particles, discarding the supernatant, and then drying the particles for a baking time at a certain temperature above the phase transition temperature to prepare silica particles;   (a-3) the step of reacting EDC/NHS materials with a material having an amine group and a thiol group, under ultrasound application to fix ABT on the surface of silica particles, thereby preparing the dispersed solution of ABT-fixed silica particles; and   (a-4) washing the dispersed solution of ABT-fixed silica particles as prepared in said step (a-3) with ethanol and chloroform through the centrifuge procedure to prepare the solution to be used in the Langmuir-Blodgett method, thereby surface-modifying silica particles.   
     
     
         10 . The method for manufacturing the substrate of the analytical sensor according to  claim 1 , wherein said step (b) comprises:
 (b-1) spreading the dispersed solution of silica nanoparticles, which are surface-modified with the organic molecule having the organic functional groups and dispersed in the organic solvent, on the water surface;   (b-2) placing a barrier on said water surface to allow silica particles to gather as the form of the thin film; and   (b-3) transferring silica particles in the form of the film to the gold substrate.   
     
     
         11 . The method for manufacturing the substrate of the analytical sensor according to  claim 10 , wherein the transition pressure applied to said barrier is about 35-45 mN/m. 
     
     
         12 . The method for manufacturing the substrate of the analytical sensor according to  claim 9 , wherein said nanoparticles are silica particles. 
     
     
         13 . The method for manufacturing the substrate of the analytical sensor according to  claim 9 , wherein said organic functional group is a thiol group. 
     
     
         14 . The method for manufacturing the substrate of the analytical sensor according to  claim 9 , wherein said organic solvent is chloroform. 
     
     
         15 . A nanofilm prepared according to a method for manufacturing a substrate of an analytical sensor, said method comprising:
 (a) preparing a dispersed solution of nanoparticles by surface-modifying said nanoparticles with an organic functional group and dispersing such surface-modified nanoparticles in a volatile organic solvent;   (b) preparing a single layer film of the nanoparticles surface-modified with said organic functional group using said dispersed solution of nanoparticles on the basis of the Langmuir-Blodgett method, and then transferring said single layer film of nanoparticles to the substrate; and   (c) coating the substrate to which said single layer film of nanoparticles is transferred with a metal thin film by means of the vacuum vapor deposition.   
     
     
         16 . A nanostructure prepared by using the nanofilm prepared according to a method for manufacturing a substrate of an analytical sensor, said method comprising:
 (a) preparing a dispersed solution of nanoparticles by surface-modifying said nanoparticles with an organic functional group and dispersing such surface-modified nanoparticles in a volatile organic solvent;   (b) preparing a single layer film of the nanoparticles surface-modified with said organic functional group using said dispersed solution of nanoparticles on the basis of the Langmuir-Blodgett method, and then transferring said single layer film of nanoparticles to the substrate; and   (c) coating the substrate to which said single layer film of nanoparticles is transferred, with a metal thin film by means of the vacuum vapor deposition.   
     
     
         17 . A substrate for measuring the surface plasmon resonance as prepared according to a method for manufacturing a substrate of an analytical sensor, said method comprising:
 (a) preparing a dispersed solution of nanoparticles by surface-modifying said nanoparticles with an organic functional group and dispersing such surface-modified nanoparticles in a volatile organic solvent;   (b) preparing a single layer film of the nanoparticles surface-modified with said organic functional group using said dispersed solution of nanoparticles on the basis of the Langmuir-Blodgett method, and then transferring said single layer film of nanoparticles to the substrate; and   (c) coating the substrate to which said single layer film of nanoparticles is transferred, with a metal thin film by means of the vacuum vapor deposition.   
     
     
         18 . The method for manufacturing the substrate of the analytical sensor according to  claim 9 , wherein in step a-1, the organic molecule comprising silicon is tetraethylorthosilicate (TEOS). 
     
     
         19 . The method for manufacturing the substrate of the analytical sensor according to  claim 9 , wherein in step a-3, the material having an amine group and a thiol group is aminobenzothiol (ABT).