Hyperphotosynthetic Organisms

Abstract

The present disclosure identifies pathways and mechanisms to confer improved industrial fitness on engineered organisms. It also discloses engineered organisms having improved industrial fitness. Synthetic biologic engineering modules are disclosed that provide for light capture, carbon dioxide fixation, NADH production, NADPH production, thermotolerance, pH tolerance, flue gas tolerance, salt tolerance, nutrient independence and near infrared absorbance. The disclosed engineered organisms can include one or more of these modules. Also provided are methods of using the engineered organism to produce carbon-based products of interest, biomass or pharmaceutical agents.

1 - 55 . (canceled) 
     
     
         56 . An engineered photosynthetic cell comprising an engineered nucleic acid encoding a catalase protein. 
     
     
         57 . The engineered photosynthetic cell of  claim 56 , wherein said catalase protein is KatG. 
     
     
         58 . The engineered photosynthetic cell of  claim 56 , wherein said engineered nucleic acid comprises a katG gene. 
     
     
         59 . The engineered photosynthetic cell of  claim 58 , wherein said katG gene is from  Synechocystis  sp. PCC 6803. 
     
     
         60 . The engineered photosynthetic cell of  claim 56 , wherein said engineered photosynthetic cell exhibits improved tolerance to SO 2 . 
     
     
         61 . The engineered photosynthetic cell of  claim 56 , wherein said engineered photosynthetic cell is a cyanobacterial cell. 
     
     
         62 . A method to produce a carbon-based product of interest, comprising culturing an engineered photosynthetic cell of  claim 56  in the presence of CO 2  and light under conditions suitable to produce the carbon-based product of interest. 
     
     
         63 . The method of  claim 62 , wherein said engineered photosynthetic cell is used for conversion of carbon dioxide and light into a carbon-based product of interest.