Production of Transgenic Avians Using Improved Retroviral Vectors

Abstract

A transgenic avian containing in its genome an exogenous nucleotide sequence which includes a promoter component and a vector with reduced promoter interference wherein the exogenous nucleotide sequence is integrated into the genome and the avian.

What is claimed is: 
     
         1 . A method of producing an exogenous protein comprising producing a transgenic avian having a nucleotide sequence comprising a self-inactivation (SIN) vector having no selectable marker and a promoter component operably linked to an exogenous coding sequence, wherein the exogenous coding sequence is expressed in oviduct cells of the avian; and obtaining the exogenous protein from egg white of the avian. 
     
     
         2 . The method of  claim 1 , wherein the exogenous coding sequence encodes a human protein. 
     
     
         3 . The method of  claim 1 , wherein the exogenous coding sequence encodes a therapeutic protein. 
     
     
         4 . The method of  claim 1 , wherein the promoter component comprises a functional promoter sequence of a promoter selected from the group consisting of avian ovalbumin promoter component, avian ovomucoid promoter component, avian lysozyme promoter component and avian conalbumin promoter component. 
     
     
         5 . The method of  claim 1 , wherein the avian is selected from the group consisting of a chicken, a turkey and a quail. 
     
     
         6 . The method of  claim 1 , wherein the self-inactivation vector comprises nucleotide sequences set forth in SEQ ID NOs: 25, 26 and 29 or nucleotide sequences having at least 90% identity thereto. 
     
     
         7 . The method of  claim 1 , wherein the promoter component comprises a nucleotide sequence having at least 90% identity to the sequence set forth in 5209 through 8311 of SEQ ID NO:22. 
     
     
         8 . A transgenic avian containing in its genome an exogenous nucleotide sequence comprising a promoter component and a self-inactivation (SIN) vector having no selectable marker, wherein the avian produces an exogenous protein. 
     
     
         9 . The transgenic avian of  claim 8 , wherein the promoter component comprises a functional promoter sequence of a promoter selected from the group consisting of avian ovalbumin promoter component, avian ovomucoid promoter component and avian lysozyme promoter component. 
     
     
         10 . The transgenic avian of  claim 8 , wherein the avian is selected from the group consisting of a chicken, a turkey and a quail. 
     
     
         11 . The transgenic avian of  claim 8 , wherein the exogenous nucleotide sequence comprises nucleotide sequences set forth in SEQ ID NOs: 25, 26 and 29 or nucleotide sequences having at least 90% identity thereto. 
     
     
         12 . The transgenic avian of  claim 8 , wherein the exogenous nucleotide sequence comprises a nucleotide sequence having at least 90% identity to the sequence set forth in 5209 through 8311 of SEQ ID NO:22.