Native potato protein isolates

Abstract

The invention relates to a process for native potato protein isolation, to native potato protein isolates, to the use thereof, and to a food product comprising a native potato protein isolate. The invention provides a novel isolation process for obtaining highly pure native potato protein isolates having a glycoalkaloid concentration of less than 150 ppm.

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         15 . Native potato protein isolate obtainable by a process comprising
 subjecting potato fruit juice to a flocculation by a divalent metal cation at a pH of 7-9:   centrifuging the flocculated potato fruit juice, thereby forming a supernatant;   subjecting the supernatant to expanded bed adsorption chromatography operated at a pH of less than 11 and a temperature of 5-35° C. using an adsorbent capable of binding potato protein, thereby adsorbing the native potato protein to the adsorbent; and   eluting at least one native potato protein isolate from the adsorbent with an eluent.   
     
     
         16 . Native potato protein isolate according to  claim 15  having an isoelectric point above 4.8, a molecular weight of more than 4 kDa, and a glycoalkaloid concentration of less than 150 ppm. 
     
     
         17 . Native potato protein patatin isolate according to  claim 15  having an isoelectric point of below 5.8, preferably of 4.8-5.5, a molecular weight of more than 30 kDa, preferably more than 35 kDa, and a glycoalkaloid concentration of less than 150 ppm. 
     
     
         18 . Native potato protein protease inhibitor isolate according to  claim 15  having an isoelectric point above 5.5, preferably above 5.8, a molecular weight of below 35 kDa, preferably of 4-30 kDa, and a glycoalkaloid concentration of less than 150 ppm. 
     
     
         19 . Native potato protein isolate according to  claim 15 , having a water solubility of more than 90%, preferably more than 95% at a pH of 7 and a temperature of 25° C. 
     
     
         20 . Native potato protein isolate according to  claim 15 , having a protein content of more than 75%, preferably more than 80%, more preferably more than 90%, and even more preferably more than 95%. 
     
     
         21 . Native potato protein isolate according to  claim 15 , wherein the amount of sulphite is less than 10 ppm. 
     
     
         22 . Food product comprising a native potato protein isolate according to  claim 15 . 
     
     
         23 . Personal care product comprising a native potato protein isolate according to  claim 15 . 
     
     
         24 . Use of a native potato protein patatin isolate according to  claim 17  as a gelling agent, an emulsifier or foaming agent in a food product. 
     
     
         25 . Use of a native protein protease inhibitor isolate according to  claim 18  as a gelling agent or a foaming agent in a food or industrial product. 
     
     
         26 . Use of native potato protein isolate according to  claim 17  as a source of pharmaceutical and therapeutic enzymes.