Pharmaceutical compositions containing botulinum neurotoxin

Abstract

This invention relates to the use of a composition comprising a polysaccharide and a botulinum toxin for reducing a skin wrinkle. In some embodiments, the polysaccharide comprises disaccharides. In some embodiments, the average molecular weight of a disaccharide unit of the polysaccharide is between about 345 D and about 1,000 D.

I claim: 
     
         1 . A device comprising at least two chambers, wherein a first chamber comprises an acidic solution of Botulinum toxin, and a second chamber comprises a buffering solution, wherein the buffering solution is present in an amount sufficient to raise the pH of the acidic botulinum toxin solution to at or near physiological pH after mixing. 
     
     
         2 . The device of  claim 1 , wherein the pH of said acidic solution of Botulinum toxin is about 3.0 to about 6. 
     
     
         3 . The device of  claim 1 , wherein said acidic solution of Botulinum toxin comprises any combination of one or all of immunotypes A-G. 
     
     
         4 . The device of  claim 1 , wherein said acidic solution of Botulinum toxin comprises about 1 to about 2000 LD 50  units of Botulinum toxin. 
     
     
         5 . A method of preparing a pharmaceutical formulation of Botulinum toxin comprising combining an acidic solution of Botulinum toxin and a buffering solution in a device of  claim 1 , wherein the pH of said botulinum toxin formulation after mixing is at or near physiological pH.