Microplate with transparent base

Abstract

The present invention relates to an improved microplate which is constructed of at least one frame part and at least one bottom part, where the bottom part has a maximum thickness of 500 μm.

1 - 20 . (canceled) 
     
     
         21 . A method for producing a microplate for use in analysis of samples, the method comprising:
 inserting a non-porous, transparent film having a thickness of less than about 200 μm into an injection mold; and   injection molding a frame and at least 96 sample cuvettes onto the film at a temperature and pressure which is effective for attachment of the film onto each cuvette such that the film forms the bottom of each cuvette with a fluid tight seal to each cuvette, and the cuvettes being arrayed in the frame.   
     
     
         22 . The method of  claim 21 , wherein the cuvette and the frame are formed of a material selected from the group consisting of acrylobutadienestyrene, polyamide, polycarbonate, polystyrene, polymethyl methacyrlate, polypropylene, styrene acrylonitirle and mixtures thereof, and the film is formed of a material selected from the group consisting of acrylobutadienestyrene, polyamide, polycarbonate, polystyrene, polymethyl methacrylate, polypropylene, styrene acrylonitirle, polyester, cellulose, cellulose derivatives, regenerated cellulose and mixtures thereof. 
     
     
         23 . The method of  claim 22  wherein the frame and cuvettes film are injection molded onto the film at a temperature in the range of from 200° C. to 300° C. 
     
     
         24 . The method of  claim 21  wherein the frame and cuvettes are injected molded onto the film at a temperature in the range of from 200° C. to 300° C. 
     
     
         25 . The method of  claim 22 , wherein the frame and the cuvettes further include at least one heat conducting material which increases the thermal conductivity of the frame and cuvettes over the thermal conductivity of the frame and cuvettes without the heat conducting material. 
     
     
         26 . The method of  claim 21 , wherein the film is formed of a material selected from the group consisting of acrylobutadienestyrene, polyamide, polycarbonate, polystyrene, polymethyl methacrylate, polypropylene, styrene acrylonitirle, polyester, cellulose, cellulose derivatives, regenerated cellulose and mixtures thereof. 
     
     
         27 . The method of  claim 21 , wherein the film is held in the injection mold using electrostatic charging of one or more of the film, and the injection mold. 
     
     
         28 . The method of  claim 21 , wherein the film is pretreated in one of a corona process, a plasma process, or is activated using an adhesion promoter. 
     
     
         29 . A method for producing a microplate for use in analysis of samples, the method comprising:
 inserting a transparent film having a thickness of less than about 200 μm into an injection mold; and   injecting a plasticized molding compound at a temperature in the range of from 200° C. to 300° C. into the injection mold in which the film has been inserted, thereby forming a frame and at least 96 cuvettes arrayed in the frame, wherein the frame and cuvettes attach to the film such that the film forms the bottom of each cuvette with a fluid tight seal to each cuvette.   
     
     
         30 . The method of  claim 29 , wherein the cuvette and the frame are formed of a material selected from the group consisting of acrylobutadienestyrene, polyamide, polycarbonate, polystyrene, polymethyl methacyrlate, polypropylene, styrene acrylonitirle and mixtures thereof, and the film is formed of a material selected from the group consisting of acrylobutadienestyrene, polyamide, polycarbonate, polystyrene, polymethyl methacrylate, polypropylene, styrene acrylonitirle, polyester, cellulose, cellulose derivatives, regenerated cellulose and mixtures thereof. 
     
     
         31 . The method of  claim 29 , wherein the frame and the cuvettes further include at least one heat conducting material which increases the thermal conductivity of the frame and cuvettes over the thermal conductivity of the frame and cuvettes without the heat conducting material. 
     
     
         32 . The method of  claim 30 , wherein the frame and the cuvettes further include at least one heat conducting material which increases the thermal conductivity of the frame and cuvettes over the thermal conductivity of the frame and cuvettes without the heat conducting material. 
     
     
         33 . The method of  claim 29 , wherein the film is formed of a material selected from the group consisting of acrylobutadienestyrene, polyamide, polycarbonate, polystyrene, polymethyl methacrylate, polypropylene, styrene acrylonitirle, polyester, cellulose, cellulose derivatives, regenerated cellulose and mixtures thereof. 
     
     
         34 . The method of  claim 29 , wherein the film is held in the injection mold using electrostatic charging of one or more of the film, and the injection mold. 
     
     
         35 . The method of  claim 29 , wherein the film is pretreated in one of a corona process, a plasma process, or is activated using an adhesion promoter. 
     
     
         36 . A method for producing a microplate for use in analysis of samples, the method comprising:
 inserting a non-porous, transparent film having a thickness of less than about 500 μm into an injection mold; and   injection molding a frame and at least 384 sample cuvettes onto the film at a temperature and pressure which is effective for attachment of the film onto each cuvette such that the film forms the bottom of each cuvette with a fluid tight seal to each cuvette, and the cuvettes being arrayed in the frame.   
     
     
         37 . The method of  claim 36 , wherein the cuvette and the frame are formed of a material selected from the group consisting of acrylobutadienestyrene, polyamide, polycarbonate, polystyrene, polymethyl methacyrlate, polypropylene, styrene acrylonitirle and mixtures thereof, and the film is formed of a material selected from the group consisting of acrylobutadienestyrene, polyamide, polycarbonate, polystyrene, polymethyl methacrylate, polypropylene, styrene acrylonitirle, polyester, cellulose, cellulose derivatives, regenerated cellulose and mixtures thereof. 
     
     
         38 . The method of  claim 37  wherein the frame and cuvettes film are injection molded onto the film at a temperature in the range of from 200° C. to 300° C. 
     
     
         39 . The method of  claim 36  wherein the frame and cuvettes are injected molded onto the film at a temperature in the range of from 200° C. to 300° C. 
     
     
         40 . The method of  claim 37 , wherein the frame and the cuvettes further include at least one heat conducting material which increases the thermal conductivity of the frame and cuvettes over the thermal conductivity of the frame and cuvettes without the heat conducting material. 
     
     
         41 . The method of  claim 36 , wherein the film is formed of a material selected from the group consisting of acrylobutadienestyrene, polyamide, polycarbonate, polystyrene, polymethyl methacrylate, polypropylene, styrene acrylonitirle, polyester, cellulose, cellulose derivatives, regenerated cellulose and mixtures thereof. 
     
     
         42 . The method of  claim 36 , wherein the film is held in the injection mold using electrostatic charging of one or more of the film, and the injection mold. 
     
     
         43 . The method of  claim 36 , wherein the film is pretreated in one of a corona process, a plasma process, or is activated using an adhesion promoter.