Means and methods for investigating nucleic acid sequences

Abstract

The invention provides improved methods for investigating nucleic acid sequences, wherein at least one additional probe is used which is specific for a (pseudo)gene variant of a target nucleic acid.

1 - 16 . (canceled) 
     
     
         17 . A probe or a probe set selected from the probes or probe sets listed in  FIG. 3A ,  3 B,  3 C or  3 D, preferably selected from the probe sets of  FIG. 3C  or  3 D. 
     
     
         18 . Mixture of nucleic acids, wherein said nucleic acids comprise at least two probes or probe sets according to  claim 17 . 
     
     
         19 . A kit for detecting the presence of at least one target nucleic acid sequence in a sample, comprising a probe or a probe set or a mixture of nucleic acids according to  claim 17 . 
     
     
         20 . A kit according to  claim 19 , wherein said at least one target nucleic acid sequence comprises a nucleic acid sequence present in a KIR locus. 
     
     
         21 . A kit according to  claim 19 , further comprising a PCR primer set comprising at least 70%, preferably at least 80%, more preferably at least 85%, more preferably at least 90%, most preferably at least 95% sequence identity to nucleic acid sequences 5′-GGGTTCCCTAAGGGTTGGA and TCTAGATTGGATCTTGCTGGCAC-3′ or the complements thereof. 
     
     
         22 - 32 . (canceled)