High Telomerase Activity Bone Marrow Mesenchymal Stem Cells, Methods of Producing the Same and Pharmaceuticals and Treatment Methods Based Thereon

Abstract

Disclosed are isolated human bone marrow mesenchymal stem cells having high telomerase activity (tBMMSCs). Also disclosed are isolated human CD34 + bone marrow mesenchymal stem cells. Also disclosed are bone marrow mesenchymal stem cells treated with a telomerase induction agent.

We claim: 
     
         1 . Isolated human bone marrow mesenchymal stem cells having high telomerase activity (tBMMSCs). 
     
     
         2 . The isolated human bone marrow mesenchymal stem cells according to  claim 1 , wherein said cells are SSEA4 + . 
     
     
         3 . Isolated human CD34 +  bone marrow mesenchymal stem cells. 
     
     
         4 . The isolated human CD34 +  bone marrow mesenchymal stem cells according to  claim 3 , wherein the stem cells are CD73 +   
     
     
         5 . The isolated human CD34 +  bone marrow mesenchymal stem cells according to  claim 3 , wherein said stem cells are CD105 +   
     
     
         6 . The isolated human CD34 +  bone marrow mesenchymal stem cells according to  claim 3 , wherein the stem cells fail to form CFU-F in plastic cultures, but adhere on mesenchymal stem cell-produced extra-cellular matrix. 
     
     
         7 . The isolated human CD34 +  bone marrow mesenchymal stem cells according to  claim 3 , where said stem cells are capable of differentiating into osteoblasts, adipocytes, and chondrocytes. 
     
     
         8 . Isolated aspirin treated bone marrow mesenchymal stem cells. 
     
     
         9 . A method of increasing telomerase activity in CD34 −  human bone marrow mesenchymal stem cells comprising: contacting human bone marrow messenchymal stem cells with an effective amount of a telomerase inducing agent. 
     
     
         10 . The method of  claim 7 , wherein the telomerase inducing agent is aspirin. 
     
     
         11 . A method of isolating high telomerase bone marrow mesenchymal stem cells comprising:
 culturing a sample of bone marrow derived all nuclear cells on a plastic substrate;   removing cells that do not adhere to the substrate;   culturing any cells in the medium on an BMMSC-ECM coated medium;   collecting colonies forming attached cells on the BMMSC-ECM medium.   
     
     
         12 . A method of treating systemic lupus erythematosus, comprising administering to the patient a therapeutically effective amount of isolated high telomerase human bone marrow mesenchymal stem cells.