Plant Genome Sequence and Uses Thereof

Abstract

The present invention is in the field of plant biochemistry and genetics. More specifically the invention relates to nucleic acid molecules from plant cells, in particular, genomic DNA sequences from rice plants and nucleic acid molecules that contain markers, in particular, single nucleotide polymorphism (SNP) and repetitive element markers. In addition, the present invention provides nucleic acid molecules having regulatory elements or encoding proteins or fragments thereof. The invention also relates to proteins and fragments of proteins so encoded and antibodies capable of binding the proteins. The invention also relates to methods of using the nucleic acid molecules, markers, repetitive elements and fragments of repetitive elements, regulatory elements, proteins and fragments of proteins, and antibodies, for example for genome mapping, gene identification and analysis, plant breeding, preparation of constructs for use in plant gene expression, and transgenic plants.

1 .- 15 . (canceled) 
     
     
         16 . A transformed plant comprising a nucleic acid molecule which comprises:
 (a) an exogenous promoter region which functions in a plant cell to cause the production of an mRNA molecule, which is linked to   (b) a structural nucleic acid molecule, wherein said structural nucleic acid molecule comprises a nucleic acid sequence, wherein said nucleic acid sequence shares between 100% and 90% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 69,652 and complements thereof, which is linked to   (c) a 3′ non-translated sequence that functions in said plant cell to cause the termination of transcription and the addition of polyadenylated ribonucleotides to said 3′ end of said mRNA molecule.   
     
     
         17 . The transformed plant according to  claim 16 , wherein said nucleic acid sequence shares between 100% and 90% sequence identity with the complement of a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 69,652. 
     
     
         18 . The transformed plant according to  claim 16 , wherein said nucleic acid sequence is in the antisense orientation of a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 69,652. 
     
     
         19 . The transformed plant according to  claim 16 , wherein said nucleic acid sequence shares between 100% and 95% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 69,652 and complements thereof. 
     
     
         20 . The transformed plant according to  claim 19 , wherein said nucleic acid sequence shares between 100% and 98% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 69,652 and complements thereof. 
     
     
         21 . The transformed plant according to  claim 20 , wherein said nucleic acid sequence shares between 100% and 99% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 69,652 and complements thereof. 
     
     
         22 . The transformed plant according to  claim 21 , wherein said nucleic acid sequence shares 100% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 69,652 and complements thereof. 
     
     
         23 . A transformed seed comprising a transformed plant cell comprising a nucleic acid molecule which comprises:
 (a) an exogenous promoter region which functions in said plant cell to cause the production of an mRNA molecule, which is linked to   (b) a structural nucleic acid molecule, wherein said structural nucleic acid molecule comprises a nucleic acid sequence, wherein said nucleic acid sequence shares between 100% and 90% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 69,652 and complements thereof, which is linked to   (c) a 3′ non-translated sequence that functions in said plant cell to cause the termination of transcription and the addition of polyadenylated ribonucleotides to said 3′ end of said mRNA molecule.   
     
     
         24 . The transformed seed according to  claim 23 , wherein said nucleic acid sequence shares between 100% and 90% sequence identity with the complement of a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 69,652. 
     
     
         25 . The transformed seed according to  claim 23 , wherein said exogenous promoter region functions in a seed cell. 
     
     
         26 . The transformed seed according to  claim 23 , wherein said nucleic acid sequence shares between 100% and 95% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 69,652 and complements thereof. 
     
     
         27 . The transformed seed according to  claim 26 , wherein said nucleic acid sequence shares between 100% and 98% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 69,652 and complements thereof. 
     
     
         28 . The transformed seed according to  claim 27 , wherein said nucleic acid sequence shares between 100% and 99% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 69,652 and complements thereof. 
     
     
         29 . The transformed seed according to  claim 28 , wherein said nucleic acid sequence shares 100% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 69,652 and complements thereof. 
     
     
         30 . A method of growing a transgenic plant comprising
 (a) planting a transformed seed comprising a nucleic acid sequence, wherein said nucleic acid sequence shares between 100% and 90% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 69,652 and complements thereof, and   (b) growing a plant from said seed.   
     
     
         31 . A substantially purified nucleic acid molecule comprising a nucleic acid sequence, wherein said nucleic acid sequence shares between 100% and 90% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 69,652 and complements thereof. 
     
     
         32 . The substantially purified nucleic acid molecule of  claim 31 , wherein said nucleic acid molecule encodes a rice protein or fragment thereof.