Noninvasive, continuous in vitro simultaneous measurement of turbidity and concentration
Abstract
The invention provides a method of determining turbidity and concentration simultaneously a sample by irradiating the sample with a single incident wavelength and simultaneously measuring wavelength shifted (IE) and unshifted (EE) light emitted. A relative volume of light emitted from two phases may be determined, wherein the two phases comprise a first Rayleigh and Mie scattering and fluorescent phase associated with suspended particles, and a second, non-scattering but fluorescent phase associated with suspending solution. Volumes of the phases and/or concentrations of specific fluorophores or Raman active species are calculated from the volume of light emitted by the first phase relative to the total volume of light emitted from the first and second phases.
Claims
exact text as granted — not AI-modifiedWhat is claimed is:
1 . A method of simultaneously obtaining a turbidity and concentration from an in vitro sample comprising:
irradiating the sample with a single incident wavelength on a sample having a fluid and particles suspended in the fluid; simultaneously measuring wavelength shifted (IE) and unshifted (EE) light emitted from the tissue; and determining a relative volume of light emitted from two phases, wherein the two phases comprise a first Rayleigh and Mie scattering and fluorescent phase associated with suspended particles, and a second, non-scattering phase associated with the fluid, wherein the percentage of suspended particles is calculated from the volume of light emitted by the first phase (Φr) relative to the total volume of light emitted from the first and second phases (Φr+Φp), wherein the determining comprises calculating:
φ
r
/
(
φ
r
+
φ
p
)
[
5
]
φ
r
=
a
+
(
b
EE
EE
0
)
+
(
c
IE
IE
0
)
wherein
[
8
]
φ
p
=
d
+
(
e
EE
EE
0
)
+
(
f
IE
IE
0
)
[
9
]
EE
=
1
+
2
φ
p
+
3
φ
r
[
6
]
IE
=
4
+
5
φ
p
+
6
φ
r
[
7
]
and wherein EE is total elastically (unshifted) emitted light, IE is total inelastically (shifted) emitted light, 1 and 4 are the fractions of EE and IE, respectively, from the background; 2 and 5 are the fractions of EE and IE, respectively, from the fluid; 3 and 6 are the fractions of EE and IE, respectively, from the suspended particles; and 1-6 are calculated numerically using the radiative transport equation (RTE) to determine EE and IE as a function of φ r and φ p ; wherein EE o and IE o are average values of EE and IE over a calibration time period; and
wherein a-f are obtained by inverting equations [6] and [7] to express φ r and φ p in terms of EE and IE.
2 . The method of claim 1 , wherein the incident wavelength is 280-2500 nm.
3 . The method of claim 2 , wherein the incident wavelength is 632, 405, 670, 450, 785, 805 or 830 nm.
4 . The method of claim 1 , wherein the step of simultaneously measuring wavelength shifted (IE) and unshifted (EE) light emitted from the background is at a shift of 500-1800 cm-1 relative to the incident wavelength for shifted light, and at −30-+10 cm-1 for unshifted light.Cited by (0)
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